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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for Recombinant Mouse Fgf18 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the bound antibody was incubated with horseradish-peroxidase labelled subclass-specific anti-mouse IgG1 at 1:8,000 or anti-mouse IgG2c at 1:2,000 (Sigma, Aldrich). After five washes with PBS-T ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2021Quote: The levels of 32 different cytokines and chemokines in mouse sera were measured using a Milliplex MAP Mouse Cytokine/Chemokine premixed 32 Plex (Millipore), following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2020Quote: ... the plates were incubated with the mouse anti-HA antibody followed by goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich). The absorbance at 405 nm was read by the microplate reader (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse anti-Ankyrin-G (Neuromab N106/36; IF 1:200) Mouse anti-ß-actin (Sigma A5316; Western blot 1:5000), Rabbit anti-ß-actin (Cell Signaling Technologies #4907 ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Biochemistry 2021Quote: ... and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL; goat anti-mouse IgG, Atto647N, Sigma Aldrich, Germany) were mixed in one batch and offered in a volume of 2 µL to the beads from the opposite side ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Immunology 2021Quote: ... mouse monoclonal anti Rho-GDI (Santa-Cruz Biotechnology, 1:500) and mouse monoclonal anti-FLAG M2 Ab (Sigma, 1:1000), anti-ERK1/2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Protein complexes were identified by adding anti-mouse IgG agarose (and mouse IgG agarose as control, #A6531 and #A0919 respectively, both Sigma) for 1 hour at 4ºC in rotation ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (rat anti-BrdU, 1:100, Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP ...
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bioRxiv - Microbiology 2022Quote: ... the microarrays were overlaid with the VP1 proteins for 90 minutes as protein-antibody complexes that were prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Biochemistry 2023Quote: ... the microarray was overlaid with the VP1 protein for 90 minutes as a protein-antibody complex that was prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Cell Biology 2023Quote: Polyclonal antibody against the C-terminus of mouse SETX-C were generated by immunizing rabbits. Mouse cDNA encoding Setx (aa. 1900-2701) was subcloned into pET15b (Novagen) and transformed to E.coli strain BL21-CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Vimentin mouse monoclonal (1:1000, 1A4, Sigma), anti-Fibronectin rabbit polyclonal (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies to β-actin (Mouse Monoclonal, #A5441 (Sigma), 1/10000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti β-actin 1:20,000 (Sigma, A1978), rabbit anti-ubiquitin 1:1,000 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Calretinin (1:250, Cat. #: AB5054, Millipore); rabbit anti-phospho-Erk1/2 (1:250 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin (mouse, Sigma, T5168, RRID 477579). Antibodies were raised in rabbits against purified ...
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bioRxiv - Molecular Biology 2020Quote: ... Flag mouse monoclonal antibody (M2, Sigma, 1:1000), HA.11 mouse monoclonal antibody (16B12 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-insulin (1:350, Sigma, #K36AC10), mouse monoclonal anti-proinsulin (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-catenin ascites fluid (C7082, Sigma); rabbit Ab to α-catenin (ab2981 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-α tubulin (T9026, Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... or β-actin (mouse monoclonal AC15, Sigma-Aldrich). Proteins were visualized with goat anti-mouse or goat anti-rabbit IRDye-800 or - 680 secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-SC-35 (1:2500, Sigma, #S4045), rabbit anti-HNRNPA1 (1:2500 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse monoclonal TUBA4A (Sigma-Aldrich, T5168, 1:4000), GAPDH (14C10 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal y-Tubulin (cat. No. T6557, Sigma), mouse monoclonal SF3B1 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... or 10 µg of mouse IgG (Sigma-Aldrich) in 200 µl of PBS + 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Tubulin (B-5-1-2, Sigma), mouse anti-CHC (X22 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-RAB10 (1:1000; Sigma; SAB53000028), rabbit polyclonal anti-RAB7 (1:1000 ...