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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/mL epidermal growth factor (EGF) (Upstate Biochem) and 100 ng/mL dexamethasone (Sigma Aldrich). The T47D cells were maintained in McCoy’s medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... 8 μg/ml aprotinin) with 100 U/ml hexokinase (from Saccharomyces cerevisiae Type F-300; Sigma).
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bioRxiv - Microbiology 2021Quote: ... with 5 ml of RPMI 1640 media (supplemented with 100 U/ml Pen-Strep (Sigma, UK), 200 mM L-glutamine (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... 100 µg/ml cyclohexamide and 1 tab/5 ml SigmaFast protease inhibitor cocktail with EDTA (Sigma). One hemisphere was used per replicate ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μg/ml aprotinin] with 100 U/ml hexokinase (from Saccharomyces cerevisiae type F-300; Sigma) for 10 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... 8 µg/ml aprotinin) with 100 U/ml hexokinase (from Saccharomyces cerevisiae Type F-300; Sigma) for 10 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... were incubated with 15 μg/ml TRITC-transferrin or 100 μg/ml acridine orange (A6014, Sigma) in serum free medium during 15 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and 100 mg/mL streptomycin) containing 50 ng/mL of phorbol myristate acetate (PMA; Sigma-Aldrich), 5 μg/mL ionomycin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... ampicillin (50 µg mL-1) (Polfa Tarchonin) and cyclocheximide (100 µg mL-1) (Sigma-Aldrich/Merck) were supplemented to the media as selective agents.
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.22-micron filtered seawater + penicillin (100 unit/mL) and streptomycin sulfate (0.1 mg/mL) (Sigma P4333A). Injected embryos were checked for developmental abnormalities and mortality every six hours until fixation.
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bioRxiv - Immunology 2024Quote: ... 10% fetal bovine serum and 100 U/mL penicillin and 0.1 mg/mL streptomycin (Sigma-Aldrich). Paired sample types were serially diluted in duplicate in cell medium ...
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bioRxiv - Physiology 2023Quote: ... PEG-SOD (100 U/ml) or PEG-catalase (1000 U/ml) (Sigma-Aldrich, St. Louis, MO) were administered in the blood perfusate which provides about 30 minutes of equilibration and remains throughout the experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 units/mL Pen/Strep antibiotic and Insulin 5 μg ml−1 (Sigma, Saint Louis, USA) at 37 °C in a balanced air humidified incubator with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Cell Biology 2019Quote: ... Sections were washed again and then mounted in 90% glycerol in PBS with 2.5 mg/mL p-Phenylenediamine (Sigma-Aldrich). Antibodies used were ...
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bioRxiv - Cancer Biology 2020Quote: ... PSCs were isolated from β-actin-GFP mice (006567) as previously described (Apte, 2011; Danai et al., 2018): 3mL of 1.3mg/mL cold collagenase P (Sigma 11213865001) and 0.01mg/mL DNAse (Sigma D5025 ...
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bioRxiv - Microbiology 2021Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma-Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
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bioRxiv - Biophysics 2020Quote: ... 25 µl cell lysate was added to 125 µl ALP substrate consisting 1 mg/ml p-nitorphenyl phosphate (Sigma Aldrich), 0.1 M diethanolamine ...
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bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... washed twice in 1x PBS and resuspended in 2 ml co-IP lysis buffer (50 mM Tris-HCl pH 7.5, 1% Nonidet P 40 Substitute (Sigma #74385), 0.25% deoxycholate Na ...
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bioRxiv - Microbiology 2020Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma–Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then washed extensively with PBS-BSA and finally mounted onto a clean glass slide using 10mg/ml p-phenylenediamine (Sigma) in 90% glycerol which is already supplemented with DAPI (20ug/ml) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% P/S) and incubated for 45 min with antibodies and 10 min with DAPI (2μg ml−1, Sigma-Aldrich), always on ice ...
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bioRxiv - Microbiology 2021Quote: ... Viruses were amplified on MDCK cells cultured in serum-free DMEM containing 0.5 μg/mL L-1-p-Tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma–Aldrich). Stocks were titrated by plaque assays on MDCK cells.
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bioRxiv - Genetics 2020Quote: ... Bead-beating was done in an Axygen 1.1 ml plate (P-DW-11-C-S) with 250 μl of acid washed 425–600 μm beads (Sigma G8722) per well ...
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bioRxiv - Microbiology 2021Quote: ... S2 or S2 P-mutant subunits in the presence of increasing doses (0.25-1 ng/ml) of LPS (Sigma, USA). Then ...
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bioRxiv - Cell Biology 2020Quote: ... and finally mounted in the anti-fade buffer (90% glycerol in PBS plus 2.5 mg/ml p-Phenylenediamine (Sigma-Aldrich)).
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested at 300 × g for 5 min and resuspended in 10 volumes of warm 1xPBS (P) or 1xPBS containing 1 mg/ml TPCK-Treated Trypsin (Sigma) (T) ...
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bioRxiv - Microbiology 2021Quote: ... The enzyme reaction was started by the addition of 0.16 ml of 6 mM p- nitrophenyl β-D-glucuronide (Sigma) to the broken cells and stopped by the addition of 0.4 ml of 1.0 M NaCO3 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were then washed extensively with PBS-BSA and finally mounted onto a clean glass slide using 10 mg/ml p-phenylenediamine (Sigma) in 90% glycerol ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of deproteinized sample were mixed with a freshly made 20 mg/ml solution of p-dimethyl-amino-benzaldehyde (Ehrlich’s reagent; Sigma # 109762) in glacial acetic acid at room temperature to allow for the formation of a yellow colored product ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was washed twice with 0.1 % BSA-PBS and covered with mounting medium [1 mg/mL p-phenylenediamine (Sigma-Aldrich) in a mixture of 90% glycerol and PBS] and 24 mm × 60 mm coverslip (Matsunami Glass IND. ...
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bioRxiv - Bioengineering 2019Quote: ... Control monolayers (n=9) of 1 x 106 cells plated on 10 μg/mL collagen type I (Sigma) were also incubated at 37° C for 7 days in α-MEM ...
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bioRxiv - Biophysics 2020Quote: ... and 1065 μL N-hydroxyethylacrylamide monomers at 65 mg/mL in deionized water (HEA, Sigma #924-42-5) were mixed together in a 15 mL Eppendorf tube 45–47 ...
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bioRxiv - Cell Biology 2022Quote: ... A confocal dish (cellvis, D35-10-1-N) was incubated with 0.1 mg/mL poly-L-lysine (Sigma) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... the cover slides were treated with 1 mg/ml N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sigma) and exposed to ultra-violet (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... for 45 seconds and mixed with 10 mL/gram of 4% (w/v) N-lauroyl-sarcosine (sarkosyl, Sigma), 2 U μl-1 homemade Benzonase and 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL progesterone (160-24511, Wako, Japan) and 25 μM N-acetyl-L-cysteine (A7250, Sigma-Aldrich)) to remove the mineral oil and M2 medium and seeded on a μ-slide 8 well (80826 ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were propagated at 37 °C O/N in 5 mL Luria-Bertani (LB) broth (Sigma-Aldrich) supplemented with 50 mg/mL kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... This pellet was resuspended in ~1.5 ml of 10 mM HEPES + 0.1% (w/v) N-Lauryl sarcosine sodium salt (Sigma) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... culture media was replaced by culture media supplemented with 1 mg/mL MTT reagent (Sigma, cat n° M5655). MTT reduction to formazan crystals by cellular oxidoreductases were allowed to form by incubating the cells in a 5% CO2 gas-equilibrated humidified incubator at 37°C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: Lysis was performed in 4 mL of 0.01 % n-dodecyl-beta-maltoside in 60 mM triethyl ammonium bicarbonate (TEAB, pH 8.5, Sigma) at 95 °C in a PCR cycler with a lid temperature of 110 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... which were then derivatized by adding 100 μl 90% N,O-bis(trimethylsilyl)-trifluoroacetamide-10% tetramethylsilane (TMS) (Sigma, St. Louis, MO) and 200 μl anhydrous pyridine (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... filled with pre-heated (to 37 °C) CUBIC-L solution (10% w/w N-butyldiethanolamine, 10%w/w triton X-100, in H2O, Sigma Aldrich) and kept at 37 °C under continuous shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... hearts were transferred in 50% glycerol for 1 h and then mounted in anti-fade mounting media solution containing 100% glycerol + 20%(w/v) n-propyl gallate (Sigma P3130).
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... clean up and trypsinisation was performed on 50-100 mg of frozen tissue (n=4/group) was homogenised in 500 μL CellLytic MT cell lysis buffer (Sigma, C3228) prior to removal of lipid and other contaminants using the ReadyPrep 2D cleanup Kit (Biorad ...
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bioRxiv - Plant Biology 2022Quote: ... NEROSs-RFP-Flag and GFP were transiently expressed in 4-week-old tobacco (N. benthamiana) leaves and the expression was induced using 100 μM β-Estradiol (cat. no: E8875-1G; Sigma). Leaves were dark-adapted for 20 min before chlorophyll fluorescence and ETR were measured at 20°C ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mg Collagenase P (Sigma Aldrich) in 10 ml Gey’s solution (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were blotted onto Immobilon-P (Millipore) with a semi-dry blotting apparatus (TRANS-BLOT SD ...