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Citations for Millipore Sigma :
3351 - 3400 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... -lys medium (Table S2) was prepared and enriched by adding 30 mg/L of the following isotopes: (1) L-Lysine (Sigma-Aldrich) for “light” (L ...
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bioRxiv - Cell Biology 2020Quote: ... DPSCs were cultured in the mineralization-inducing fluid (5% FBS, 10 mmol/L β-sodium glycerol phosphate, Sigma; 10−7mol/L dexamethasone, Sigma; 50 ng/mL ascorbic acid, Sigma) containing 0 ...
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bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
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bioRxiv - Cell Biology 2024Quote: ... Inhibition of NO in cell system was achieved by treating the cells with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, (1 mM) (#N5751; Sigma Aldrich) which is an analog of arginine ...
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bioRxiv - Physiology 2024Quote: ... quail were placed in glass chambers (3.3 L at 3 weeks; 8.0 L at 8 weeks) ventilated with dried (via drierite; Sigma-Aldrich, Stockholm, Sweden) atmospheric air at least 30 minutes before measurement ...
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bioRxiv - Immunology 2023Quote: ... isotonic (292 mOsm/l) RPMI+ medium was used in which some NaCl was replaced with L-glucose (G5500-5G, Sigma-Aldrich), an inert osmolyte.
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bioRxiv - Microbiology 2023Quote: ... each slide was prepared with 9 μL of Galleria homogenate to which 1 μL calcofluor white stain was added (calcofluor white M2R, 10 mg/L, Evans blue, 5 mg/L, Sigma Aldrich). Images were taken with a Leica M80 Stereomicroscope at a 60X magnification ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 mM L-Arg and L-Glu by overnight dialysis then concentrated using a 10 kDa Centriprep concentrator (Millipore, UFC901024). The concentrated protein was passed through a pre-equilibrated size exclusion column (Cytiva Custom Superdex TM 75 Increase HiScale TM 16/40 ...
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bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
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bioRxiv - Bioengineering 2022Quote: ... the cortical hemispheres were rinsed in GBSS and then transferred to a petri dish previously coated with 15 μg L-1 of Poly-L-ornithine (Sigma Aldrich). Each cortex was then cut into small pieces in Modified Eagle’s Medium (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... were given an intraperitoneal injection of anesthetic (working stock: 9 g l-1 Nembutal, Sigma-Aldrich Cat# P37610; 20.8 g l-1 MgSO4, Sigma-Aldrich Cat# 63138 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nω-Nitro-L-arginine (L-NNA) (Catalog # N5501) and Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) (Catalog # N5751) were both obtained from Millipore Sigma and solubilized in 1M HCL and dH2O ...
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bioRxiv - Microbiology 2023Quote: ... The double auxotrophic Mtb mc26206 strain (H37Rv ΔpanCD ΔleuCD)27 was grown in the minimal medium with an additional 50 mg l-1 L-leucine (Sigma, L8000) and 24 mg l-1 pantothenic acid (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Germany) or a mix of three sugars (referred to as 3C; 1 g/L starch, 1 g/L cellobiose (Sigma-Aldrich) and 1 g/L glucose) ...
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bioRxiv - Microbiology 2023Quote: ... AtSPHERE collection)36 were routinely grown on TSA (30 g/L tryptic soy broth and 15 g/L of agar, both Sigma-Aldrich) at 25 °C – 28 °C or TSB liquid medium (30 g/L tryptic soy broth) ...
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bioRxiv - Physiology 2023Quote: ... and endothelium-dependent and -independent vasorelaxation responses were determined with acetylcholine (10-9 to 10-5 mol/L) and sodium nitroprusside (10-9 to 10-5 mol/L) respectively (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... Where indicated, glucose-free cultures were hexose-supplemented using D-glucose (2 g/L) (Fisher, D-galactose (2 g/L) (Sigma-Aldrich), or D-mannose (125 µM ...
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bioRxiv - Microbiology 2024Quote: ... United Kingdom) supplemented with 1·68 mg/L penicillin G sodium salt and 1·332 g/L streptomycin sulfate (both Sigma Aldrich). For several samples ...
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bioRxiv - Systems Biology 2024Quote: Protein solutions (supernatant from homogenized tissues and cells) were dissolved in 100 mmol/L ABC with 8 mol/L urea (Sigma-Aldrich). The proteins were reduced with TCEP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... or Envelope T9I expression constructs (in total 3 µg DNA/well) in 6-well format by using per well 10 µL 1x polyethyleneimine-hydrochlorid (PEI) (Sigma-Aldrich, #764965-1G, 1 mg/mL in H2O) and 500 µL Opti-MEM reduced serum media (Gibco ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... For metabolic labelling experiments with m+4Met under all conditions mPSC basal medium was generated with 0.2 mM L-Methionine- and L-Cysteine-free DMEM (Sigma or Life Technologies) supplemented with m+4Met ([methyl-13C,d3] ...
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bioRxiv - Microbiology 2019Quote: ... mutans B04Sm5 was cultivated in 50 mL BHI medium supplemented with 1% glucose and 200 mg/L isotope-labeled [13C1] L- or D-leucine (Sigma-Aldrich, USA). The compounds were extracted and isolated using the method described above ...
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bioRxiv - Cell Biology 2022Quote: ... media contained 0.17 g/L yeast nitrogen base without ammonium sulfate (Conda Pronadisa #1553) and 1 g/L of monosodium glutamic acid (Sigma-Aldrich #G1626) instead of yeast nitrogen base with ammonium sulfate ...
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bioRxiv - Plant Biology 2022Quote: ... Inoculum was prepared to contain approximately 8,000 mycelium fragments ml-1 in sterile dH2O with 5 g l-1 glucose and 150 µl l-1 Tween 20 (Sigma-Aldrich, Germany). Two to four days prior to inoculation ...
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bioRxiv - Neuroscience 2020Quote: ... Pyruvate (40 mg/L; P2256) and L-Glutamine (2 mM; G7513) (all reagents were acquired from Sigma-Aldrich, St. Louis, MO, USA), and pH calibrated to 7.4 following 37 °C non-CO2 incubation ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat ...
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bioRxiv - Biochemistry 2020Quote: 15 g/L of BBPC was hydrolyzed by Alcalase®2.4 L (from Bacillus licheniformis, Subtilisin A, P4860, Sigma Aldrich, MO, USA) with different enzyme concentration (0.1% v/v to 0.5% v/v ...
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bioRxiv - Neuroscience 2019Quote: Surgery was done outside of the water while the fish was anesthetized and perfused through its mouth (MS-222 200 mg l−1, NaHCO3 400 mg l−1, Cat A-5040 and Cat S-5761, Sigma-Aldrich, USA) as described by Vinepinsky et al.[23] ...
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bioRxiv - Cell Biology 2021Quote: ... media contains 0.17 g/L yeast nitrogen base without Ammonium Sulfate (Conda Pronadisa #1553) and 1 g/L of monosodium glutamic acid (Sigma-Aldrich #G1626) instead of yeast nitrogen base with ammonium sulfate ...
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bioRxiv - Neuroscience 2021Quote: ... (2) rats underwent UVN and received intraperitoneally one L-thyroxine (L-T4) injection (10 µg/kg, dissolved in saline, Sigma, Cat#T2501) at the end of the surgery and one injection/day during the first 3 days post-lesion (UVN-T4 group ...
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bioRxiv - Biochemistry 2022Quote: ... Bilayer lipid membranes were formed according to Muller–Rudin [70] using a solution containing 20 g/l of soybean lecithin (L-α-phosphatidylcholine Type IV–S, Sigma, USA). The surrounding solution contained 0.2 М KCl and 5 mM HEPES-Tris (Sigma) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...
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bioRxiv - Pathology 2022Quote: ... Then the leaves were soaked up using absorbent paper and transferred into another square Petri dish containing 8 g L-1 agar with 50 mg L-1 benzimidazole (Sigma-Aldrich, USA) for 24 hours and finally transferred to new Petri dishes and infected with Bgt #70.
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bioRxiv - Neuroscience 2022Quote: ... the anesthetized fish was placed in a holder on the operating table and perfused through its mouth with water and anesthetic (MS-222 200 mg/l, NaHCO3 400 mg/l 1, Cat A-5040, and Cat S-5761, Sigma-Aldrich, USA). All surgery specifics are described in detail in (39) ...
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bioRxiv - Microbiology 2023Quote: ... Netherlands) medium supplemented with 10 g l−1 sucrose and solidified with 4.2 g l−1 phytagel (Sigma-Aldrich, St. Louis, USA). The microboxes were placed in a growth chamber (Snijders Scientific B.V. ...
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bioRxiv - Physiology 2023Quote: ... eNOS-independent relaxation was tested in presence of the pharmacologic eNOS inhibitor N-nitro-L-arginine methyl ester (L-NAME, 300 μM; Sigma-Aldrich N5751) either using increasing concentrations of the NO-donor dimethylamine nonoate (DEA-NONOate ...
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bioRxiv - Microbiology 2023Quote: ... difficile R20291 carrying either pDSW1728-PtcdA::mCherry or pDSW1728 was plated on BHI containing 10 mg/L L-cysteine and 10 µg/mL thiamphenicol (Sigma, cat # T0261). After an overnight incubation at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 9 g l-1 Nembutal, Sigma-Aldrich Cat# P37610; 20.8 g l-1 MgSO4, Sigma-Aldrich Cat# 63138; 10% ethanol, Sigma-Aldrich Cat# 459836 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µM of the nonspecific nitric oxide synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME [Sigma Aldrich], n=5), or 1.0 mM P188 and 100 µM L-NAME (n=5) ...
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bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
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bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
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bioRxiv - Bioengineering 2020Quote: ... 1.36 g/L NaC2H3O2 (Sigma-Aldric, St. Louis, MO), 0.20 g/L MgCl2·6H2O (Sigma Life Science ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-coated with Poly-L-lysine (P4707, Sigma-Aldrich) and then processed with treatments as indicated ...
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bioRxiv - Bioengineering 2020Quote: ... an Entactin-collagen IV-laminin (E-C-L, Millipore) solution (10 μg/mL in sterile serum-free medium ...
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bioRxiv - Biophysics 2020Quote: ... and 1% L-Glutamine solution (200 mM, 59202C, Sigma). An Olympus IX71 with a Cairn OptoLED light source and an Olympus PlanApo 100x oil-immersion objective with a numerical aperture of 1.40 were used to view the cells ...
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bioRxiv - Biophysics 2020Quote: ... Eluant was concentrated to ∼1 mg/L (EMD Millipore Amicon Ultra-15 ...
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bioRxiv - Biophysics 2020Quote: ... and 50 mg/L DNase I (Sigma-Aldrich, USA). The membrane fraction of cell lysate was isolated by ultracentrifugation at 90000 g for 1 h at 4° C ...
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bioRxiv - Developmental Biology 2021Quote: ... and 200 μM L-ascorbic acid (Sigma Aldrich, A8960) for 10 days ...
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bioRxiv - Developmental Biology 2021Quote: ... and 200 μM L-ascorbic acid (Sigma Aldrich, A8960). Medium was supplemented with 10 μM Y-27632 (Tocris ...