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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for Biotin Z Antibody Internalization Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cooled down channels were filled with 1 mg/mL biotin labelled bovine serum albumin (BSA, Sigma-Aldrich) in T50 buffer (10 mM Tris/Cl ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule seeds were immobilized by sequential incubation with: (i) 1 mg/ml BSA-biotin (A8549; Sigma-Aldrich), (ii ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium hyaluronan (1.5MDa) was from Lifecore (Chaska, MN, USA) and hyaluronan biotin sodium salt was from Sigma. Casein blocking buffer (10× ...
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bioRxiv - Bioengineering 2020Quote: ... The unreacted biotin was removed by ultrafiltration with an Amicon column (30 KDa MWCO, Millipore, CAT: UFC5010BK).
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bioRxiv - Biophysics 2019Quote: ... the free neutravidin on the single molecule surface was concealed with saturated concentrations of biotin (Sigma-Aldrich), prior to addition of the fluorogen ...
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bioRxiv - Microbiology 2020Quote: ... RcsF-His (5mg/mL in 0.1 M MES [pH 5.5]) was incubated with Biotin-LC-Hydrazide (1.25 mM, final concentration; Sigma) and N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide (EDC ...
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bioRxiv - Neuroscience 2021Quote: ... Remaining surface biotin was cleaved by incubation with glutathione cleavage buffer (50 nM L-Glutathione (Sigma #G6013) 75 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated with an IB4-biotin conjugate (1:200, Sigma-Aldrich, Burlington, MA, USA, L2140) prepared in 5% normal goat serum (NGS)/PBST overnight at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The beads were then re-suspended in 20 μl of 2 μg/ml Atto565-biotin (Sigma-Aldrich), incubated for 5 minutes ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... a 96 well glass bottom plate was prepared by plating first biotin-BSA (Thermo) and fibronectin (Sigma), followed by neutravidin (Thermo) ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...
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bioRxiv - Plant Biology 2023Quote: ... Labelled proteins were biotinylated with 20 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described in “Labeling and click chemistry” ...
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bioRxiv - Immunology 2023Quote: ... the glass bottom of a 384-well MatriPlate was coated with 1mg/mL biotin-BSA (Sigma-Aldrich), followed by 200µg/mL NeutrAvidin (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Biotin-poly-L-lysine was produced by incubating 5 mg/mL poly-L-lysine hydrobromide (Sigma P6282) with 1.5 mM EZ-Link Sulfo-NHS-Biotin (TFS 21217 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Cell Biology 2024Quote: ... Proximity labeling was induced overnight by supplementing the medium with 10 μM Biotin (Sigma-Aldrich, cat. #B4501). The cells were lysed ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Synthetic Biology 2022Quote: ... and analysed using the WesternBreeze Chromogenic anti-mouse Kit with a primary anti-FLAG (F4042, Sigma-Aldrich) antibody at a 4000x dilution ...
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bioRxiv - Cell Biology 2021Quote: Duolink In Situ Red Starter Kit Mouse/Rabbit Proximity Ligation Assay was purchased from Millipore Sigma (DUO92101) and was performed according to the manufactures protocol using KLK10 and HTRA1 antibodies described above.
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bioRxiv - Cell Biology 2019Quote: ... The proximity ligation assay was conducted using a DuoLink In Situ Mouse/Rabbit PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: Proximity ligation assay was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Immunology 2023Quote: Cytokine quantification was determined using the MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Kit (MCYTMAG-70K-PX32, Millipore). The assay was conducted as per the manufacturer’s instructions and plates were ran on a MAGPIX instrument with xPONENT software ...
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bioRxiv - Molecular Biology 2023Quote: ... Proximity ligation was performed using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Protein-protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: PLA was performed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, DUO92101) protocol with some modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
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bioRxiv - Microbiology 2019Quote: ... The antigen-antibody complexes were visualized by the Immubilon™ chemiluminescent detection kit (Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Cancer Biology 2023Quote: ... and bound antibodies were detected using an ECL detection kit (Immobilon Western ECL, Millipore) or with the ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... or 10μg of p53 antibody (Santa Cruz, DO1) (rabbit or mouse IgG was used as a negative control (Sigma, M7023)) for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 was used in all experiments except where indicated (mouse ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies were detected by incubation for 1 h with FITC-conjugated goat anti-mouse IgG (1:40, Sigma F8264) or Alexa Fluor 568-conjugated goat anti-mouse IgG (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were incubated in primary antibody (0.15μg/ml of Mouse Anti-BrdU in 5% BSA) (Cat. no. B2531; Sigma-Aldrich, USA) overnight in a moist chamber at 4°C in the dark ...
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bioRxiv - Developmental Biology 2020Quote: ... were washed in IP buffer and incubated with primary antibody (rabbit anti-HA, anti-myc, CST or mouse anti-flag, Sigma) or normal IgG as control (mouse IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Neuroscience 2021Quote: ... One set of slices were stained with primary antibodies for mouse monoclonal anti-GFAP (G3893; Sigma-Aldrich; 1:1,000 dilution) and rabbit anti-Iba1 (019-19741 ...
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bioRxiv - Neuroscience 2021Quote: The primary polyclonal antibodies applied overnight at 4 °C were as follows: mouse anti-UBE3A 1: 600 (SAB1404508, Sigma, USA); rabbit anti-ELAVL4/HudD 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody against vinculin was purchased from HRP-conjugated antibodies against mouse IgG (NA931V; 1:10 000) and rabbit IgG (NA934V; 1:10 000) were purchased from Sigma. Immunohistochemistry was performed by Molecular Pathology (Robarts Research Institute ...
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bioRxiv - Neuroscience 2019Quote: ... and corresponding PVDF membranes were incubated overnight at 4°C with primary antibodies: mouse anti-ATXN3 (1H9) (1:2000, MAB5360, Millipore), rabbit anti-MJD [22] (1:20000) ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594-conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...