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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL protoplast was mixed with 20 μL DNA (1 – 2 μg total) and 50 μL ice-cold solution 4 (25 g of PEG 6000 or 8000 (Sigma, P2139), 1.47 g of CaCl2.2H2O ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed twice at RT with 0.1% Triton 100X in PBS and then counterstaining with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (1:5000, Sigma Millipore, #MBD0015) in PBS for five minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin, F1141, Sigma-Aldrich) tissue cultureware.
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bioRxiv - Cell Biology 2024Quote: ... gels were incubated in primary antibodies (Supplementary Table 4) diluted to 1:500 in antibody dilution buffer (2% bovine serum albumin (BSA; Sigma, A9647) in 1X PBS ...
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bioRxiv - Biophysics 2020Quote: ... β-octyl glucopyranoside and α-Cyano-4-hydroxycinnamic acid matrix were purchased from Sigma Aldrich (St. Louis, MO). Water and acetonitrile were of LC–MS grade and obtained from Honeywell (California ...
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bioRxiv - Bioengineering 2023Quote: ... Correct product masses were confirmed by MALDI mass spectrometry using the matrix α-cyano-4- hydroxycinnamic acid (Sigma).
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bioRxiv - Evolutionary Biology 2022Quote: ... resuspended in 4 mL SGDCAA (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... was dissolved in 70 µl of 0.2 M phosphate containing 6 M GnCl (pH 6.8-7.0) containing 100 molar eq of 4-mercaptophenylacetic acid (MPAA, Sigma) and added to the peptide ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Molecular Biology 2021Quote: ... free fatty acid (palmitoleic acid, Sigma-Aldrich, P9417), squalene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Periodic acid-Schiff (PAS) (periodic acid, Merck-Millipore, 100524002 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Gallic acid and Ellagic acid from Sigma-Aldrich, USA ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 3×10 minutes (or 4×15 minutes) in 0.3% Triton-X (Sigma T8787) in PBS (PBT ...
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bioRxiv - Immunology 2021Quote: ... and washed by ultracentrifugation at 4°C using a 3 kDa filter (UFC900324, Merck-Millipore) to remove imidazole ...
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bioRxiv - Bioengineering 2021Quote: Hydrogels were fixed on day 3 of culture using 4 mL of formalin (Sigma-Aldrich) followed by three PBS washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Stock solutions: 50 mg/ml 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma B8503) in 100% dimethylformamide (Sigma D4254 ...
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bioRxiv - Cancer Biology 2021Quote: ... TRCN0000107538-shLBH#3 and TRCN0000107533-shLBH#4) or a non-targeting control shRNA (Sigma, SHC002V) at MOI=5 and in the presence of 8 μg/ml polybrene ...
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bioRxiv - Bioengineering 2022Quote: Two-phase fibroblast-osteoblast ADC scaffolds (n = 3) were fixed in 4% paraformaldehyde (Sigma, Canada) for 1 hour and submerged in gradient sucrose solutions from 10% to 30% ...
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bioRxiv - Biochemistry 2021Quote: ... medium (∼3 liters) was harvested and flowed through a 4 ml Fractogel SO3- (EMD Millipore) cation exchange column at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Pups were intragastrically injected with three doses of 4-Hydroxytamoxifen (50µg) (Sigma, 68047-06-3) at P2 ...
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bioRxiv - Cell Biology 2020Quote: ... 8-week old animals were injected intraperitoneally with 3 doses of 4 mg tamoxifen (Sigma) and allowed a 2-week wash-out period ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested (5,000 x g; 20 min; 4°C; Sigma 3-16KL; rotor 11180); the spent medium was TCA-precipitated (20% w/v ...
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bioRxiv - Physiology 2020Quote: ... and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP; Sigma-Aldrich, Gillingham, UK) substrate solution (50 mg/ml BCIP in autoclaved water ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and 12.5 µl of 3 mM 4- methylumbelliferyl N-acetyl-β-D-glucosaminide (Sigma-Aldrich) followed by incubation for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... or 400µg NP-PCC (4-hydroxy-3-nitrophenylacetyl (Biosearch) conjugated to pigeon cytochrome C (Sigma)) mixed with adjuvant based on Monophosphoryl Lipid A ...
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bioRxiv - Physiology 2022Quote: ... five male or female flies (3 - 4 d old) were extracted with hexane (Sigma Aldrich) spiked with 10 μg/ mL of hexacosane (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4-triazol-3-yl)thio)acetamide) (CAS 525582-84-7) was purchased from Sigma-Aldrich. The stock solution was 110 mM in DMSO and subsequently diluted into appropriate buffer solution.
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bioRxiv - Microbiology 2024Quote: Sulfamethoxazole (IUPAC: 4-Amino-N-(5-methylisoxazol-3-yl)-benzenesulfonamide) was purchased from Sigma-Aldrich, USA ...
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bioRxiv - Neuroscience 2024Quote: Females aged 3–4 days were head-fixed to a custom mount with eicosane (Sigma) and imaged on a Movable Objective Microscope with resonant scanners (MOM ...
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bioRxiv - Genetics 2023Quote: ... Subsequently a solution (50 μL) containing 3∼4 U/μl trypsin (Sigma-Aldrich No. T8003) and 8 mM nicotinamide was added ...
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bioRxiv - Developmental Biology 2024Quote: ... stage 3 to 20 HH chicken embryos were fixed in 4% paraformaldehyde (PFA)(158127, Sigma) diluted in PBS 1X at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... This solution was prepared using 27 ml Ames + 3 ml 4% BSA (Sigma #A9418-5G) + 50 µl DNase ...
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bioRxiv - Neuroscience 2024Quote: ... 0,2% sodium taurodeoxycholate hydrate and 3 mM 4-methylumbelliferyl β-D-glucopyranosidase (Sigma-Aldrich, M3633) and incubated at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mL supplemented McIlvaine buffer (McIlvaine buffer pH 4 + 3 mM sodium taurocholate (Sigma # T4009) and 25uL 50 μg/mL 4-Methylumbelliferyl β-D-glucopyranoside (Sigma #M3633 ...
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bioRxiv - Cell Biology 2024Quote: ... The cleaved protein was collected and concentrated using Amicon Ultra-4 (3 K MWCO, Millipore), and then gel-filtrated over the Superdex 200 Increase 10/300 GL column with A50 buffer containing 1 mM DTT ...