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Citations for Millipore Sigma :
3301 - 3350 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Plasma FGF21 (pg/ml) was measured using a human FGF21 ELISA kit (Millipore, Billerica, MA). In Experiment 2 ...
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bioRxiv - Microbiology 2021Quote: ... a human IgG1 kappa standard purified from myeloma plasma (in duplicates per plate, Sigma-Aldrich) and serum samples (starting at a 1:20 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
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bioRxiv - Cancer Biology 2020Quote: HCT116 human colon adenocarcinoma cell lines were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma). Cell culture media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2021Quote: ... 2×106 cells/ml sperm were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Human PBMC were isolated from the peripheral blood of healthy volunteers using Ficoll (Sigma-Aldrich) density centrifugation in Greiner LeucoSep-tubes (Sigma ...
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bioRxiv - Genetics 2022Quote: ... the corresponding cDNA fragment of human FLCN was cloned in the pET-30 vector (Novagen) and the His-tagged fusion protein was expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... The COCs were incubated in human tubal fluid (HTF) medium (Millipore Corp; MR-070-D) with appropriate concentration of sperm obtained from 1 hour capacitation ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX, C3657, Sigma-Aldrich, St Louis, MO) was pre-solubilized in aqueous acid and diluted in carbonate coating buffer ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Bioengineering 2019Quote: Genotoxicity assay was determined using Hek 293T cells (Human embryonal kidney cells, Sigma-Aldrich, USA) according to Comet assay as previously described [31] ...
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bioRxiv - Cell Biology 2020Quote: ... pre-coated with human type IV collagen (Cat# C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day −2) ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Microbiology 2020Quote: Human foreskin fibroblasts (Hff1; ATCC® SCRC-1041™) were maintained in DMEM (Sigma-Aldrich) supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin and 100 µg/mL streptomycin.
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lines and primary human fibroblasts (MRC5) were cultured in DMEM (D6546, Sigma-Aldrich) with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by intra-peritoneal injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human liver hepatocellular carcinoma (HepG2) cells were cultured in MEM (Sigma, St. Louis, MO, USA) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (human embryonic kidney) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma D6429) containing 10% FBE ...
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bioRxiv - Neuroscience 2021Quote: ... full length human NPY peptide (10 pmol in 1x PBS, Sigma-Aldrich, Cat. No. N5017), anti-CART antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and subsequently incubated for 10 min in the presence of 5 % human serum (Sigma-Aldrich). Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs ...
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bioRxiv - Biochemistry 2021Quote: The human Hsf1 and Hsf1-GFP expression constructs were cloned into a pET vector (Novagen). Hsf1 CS mutant (C35S/C103S/C153S/C373S/C378S ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Cell Biology 2020Quote: Mouse embryonic lungs and human bronchial tissue were fixed overnight in 4% PFA (Sigma; 441244) at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... A standard curve was prepared using human IgG1 kappa purified from myeloma plasma (Sigma-Aldrich). Serial dilutions of the IgG standard (in duplicates ...
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bioRxiv - Immunology 2021Quote: The human lung adenocarcinoma A549 cells (ATCC, Manassas, VA) were grown in DMEM (Sigma-Aldrich) medium containing 10% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2020Quote: Fresh or cryopreserved human PBMC’s were isolated from heparinised whole blood by Ficoll gradient (Sigma). Cells were incubated for 1hr (37°C ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... Aβ42 levels were measured using a High Sensitivity Human Amyloid β42 ELISA Kit (Millipore, #EZHS42).
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bioRxiv - Developmental Biology 2021Quote: Sectioned tissue derived from regenerated human skin was fixed with 10% formalin solution (Sigma HT5012) for 12 minutes ...
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bioRxiv - Genetics 2022Quote: ... Human embryonic samples were fixed in 10% (w/v) neutral-buffered formalin solution (Sigma-Aldrich) and embedded in paraffin wax before sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Fibrin hydrogels were prepared by combining fibrinogen from human plasma (30 mg/mL Sigma-Aldrich) with thrombin from human plasma (1000 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... SYBR Green I (10,000× dilution; Cambrex Bioscience) and 0.3 μM human-specific primers (Sigma-Aldrich), as shown in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... Both human and mouse retinal explants were flat mounted on polytetrafluoroethylene organotypic filters (Millipore-Sigma) with the photoreceptor side down ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: Rabbit anti-human Aquaporin 4 (1:100, Millipore, AB3594), Rat anti-human CD44 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Akata cells were treated with 10 µg/ml of goat anti-human IgG (Sigma I1886) for 0 ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Pre-clinical Repository).
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bioRxiv - Microbiology 2024Quote: ... Non-specific binding sites on the bacterial cells were blocked using human IgG (Sigma, UK) for an hour and washed with flow buffer again before incubated overnight with the rabbit immune sera (1:1,000 dilution) ...
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bioRxiv - Immunology 2024Quote: ... or goat anti-human secondary HRP antibody (Sigma-Aldrich, #A0170, 1:10,000, for baboon plasma) in 1% BSA in TBS for 1 hour at room 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA constructs in a pLKO.1 vector targeting human CHMP5 were purchased from Sigma Aldrich along with 2 control non-targeting shRNAs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... filtered and used to infect human myoblasts assisted by polybrene (Sigma-Aldrich, TR-1003-G). When the culture reached 80-90% confluency ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cell lines and human primary cells were cultured with RPMI 1640 (R8758 Sigma-Aldrich) medium complemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the adding 50 µg/ml (1:100) of human plasma fibronectin (Sigma-Aldrich) for overnight incubation at 4°C.