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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl ß-D-1-thiogalactopyranoside (IPTG; Sigma Aldrich). Expression temperature was reduced to 18 °C and the cells were shaken for ∼18 h ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-vinculin (clone hVin-1, 1:2,000 for WB; V9264; Sigma-Aldrich). Secondary Alexa Fluor 680– conjugated (1:10,000 ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with antibodies against selected proteins overnight at 4°C: Anti-β-Tubulin III (β-Tub III) (Sigma, T8660, mouse, 1:2000); anti-p-Tau (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) with 1% bovine serum albumin and then incubated overnight in a purified antiserum against TH generated in rabbits (Sigma-Aldrich, Søborg, Denmark; cat#AB152) diluted 1:500 in PBS + 0.1% Triton-X overnight at 4°C ...
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bioRxiv - Zoology 2021Quote: ... cardiac troponin I (cTnI) and hyperpolarization activated cyclic nucleotide gated potassium channel 4 (Hcn4) as well as nuclei using DAPI (Sigma-Aldrich, dilution 1:1000, D9542). For SMA we used the primary antibody RRID:AB_476701 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS with 0.2%-Triton X-100 and then incubated overnight at 4 °C with primary antibodies (1:500; Anti-Tyrosine Hydroxylase Antibody Sigma⍰Aldrich, USA, catalog no. AB1542) in the same blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... The striatal sections were homogenized in 1 ml of synaptosome homogenizing buffer (320 mM sucrose, 4 mM HEPES, 1x Sigma protease inhibitor cocktail (CAT#P8340), pH 7.4 ...
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bioRxiv - Physiology 2022Quote: ... They were cultivated at a density of 3 × 105 cells/mL in 25 mm glass coverslips in a 4:1 Dulbecco modified Eagles minimal essential medium/199 medium (Sigma-Aldrich St. Louis, MO, USA); supplemented with 20% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4 °C in the following primary antibodies diluted in TBST: 1:200 dilution of anti-CRABP2 (MAB5488, EMD Millipore/Sigma Aldrich, St. Louis MO), 1:200 dilution of anti-RALDH2 (#13951-1-AP ...
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bioRxiv - Neuroscience 2023Quote: Rats were anaesthetised in a gas inhalation chamber using 4% isoflurane (Piramal Healthcare, Mumbai, India) in pure oxygen and maintained throughout surgery with urethane (1 g/kg; Sigma: St. Louis, Missouri, United States) and α-chloralose (50 mg·kg-1 ;Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and ultrapure H2O and finally incubated in a solution of poly-T ssDNA (100 nM) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (10 mM HEPES, NaCl 50 mM, pH 7, Sigma-Aldrich, St Louis, MO, USA) for 1 h at approx ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were then counter-stained with a mixture (1:250 dilution) of 4′,6-diamidino-2-phenylindole (DaPi. Cat# D9542. Sigma-Aldrich, St. Louis, MO USA) and bisbenzimide H 33342 trihydrochloride solution (Hoechst 33342 ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were incubated overnight at 4 °C with the following antibodies diluted in blocking solution: FITC conjugated PNA (L7381; 1:100; Sigma-Aldrich, St. Louis, MO, USA), anti-rhodopsin (ab98887 ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclear staining to reveal general head morphology (after Sandell et al., 2018) was performed on fixed juveniles and larvae with DAPI (1:1000, Sigma D9564; overnight at 4°C) or DRAQ5 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... ab195352, Abcam; anti-METTL14: 1:200, HPA038002, Sigma-Aldrich; anti-WTAP: 1:500, ab195380, Abcam; anti-Flag: 1:200, F3165, Sigma-Aldrich) at 4 overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and VGT3 (1 μg/ml) (J. Somogyi et al., 2004), Sst (Penisula lab., T-4103.0050: 1/2000) or PV (Sigma: P3088, 1/2000).
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bioRxiv - Systems Biology 2020Quote: ... rabbit anti-PDGFRα (1:400, gift from Prof Stallcup); goat anti-PDGFRα (1:200, R&D Systems; mouse anti-APC (CC1; 1:300, Millipore); rabbit anti-GFAP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h at RT followed by nuclear counterstaining with DAPI (1 μg/ml in 1 x PBS; Sigma-Aldrich, Germany) for 10 min at RT ...
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bioRxiv - Plant Biology 2022Quote: ... Inoculum was prepared to contain approximately 8,000 mycelium fragments ml-1 in sterile dH2O with 5 g l-1 glucose and 150 µl l-1 Tween 20 (Sigma-Aldrich, Germany). Two to four days prior to inoculation ...
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bioRxiv - Cell Biology 2022Quote: ... the PVDF membrane was blocked with PBS solution containing 5% nonfat dry milk for 1 hour before being incubated with primary antibodies : MyoD (ab203383) at 1:100,Myogenin (ab1835) at 1:250,MyHC (MAB4470) at 1:500 and actin (Sigma–Aldrich, A5316) at 1:800 ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were then transferred to Polyvinylidene fluoride membranes at 85V for 1 hour using 1 X NuPAGE Transfer Buffer [50 mL of 20 × 1 L Stock [81.6 g Bicine (Sigma-Aldrich, B3876-250G), 104.8 g Bis-Tris (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... Activity (1 U = 1 µM min-1) was calculated using a calibration curve prepared with a p-nitrophenol standard (Sigma-Aldrich, USA). Activity of the enzyme bound to the surface of each of the tested recombinants was related to the calculated activity of free purified BglC-CtDoc or BglC-AcDoc and used for quantitative comparison of the enzyme-binding capacity of the EM42 and EM371 strains displaying single-cohesin or two-cohesin scaffoldin.
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail, and 1 tablet of Millipore Sigma PhosStop (4906845001). The lysate was sonicated 2 times for 5 seconds at setting 2 using a Fisher Scientific Sonic Dismembrator Model 100 with 1 min on ice in between ...
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bioRxiv - Physiology 2021Quote: Whole liver tissues were homogenized in lysis buffer containing 20 mM Tris-HCl, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton, phosphatase (Pierce, A32957) and protease inhibitors (Sigma-Aldrich, P8340). Protein concentration was measured with BCA method (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... the primary antibody (anti-SKIV2L, Proteintech group, 11462-1-AP, 1:1000; anti-TTC37, Novus Biologicals, NBP1-93640, 1:500; anti-WDR61, Sigma-Aldrich, SAB1401852 ...
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bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked in 5% milk for 1 hour and then incubated with primary antibodies for Tubulin (CST, 1:5000) and acetylated Tubulin (Sigma, 1:5000) in PBS supplemented with 3% BSA and incubated overnight on a rolling platform at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then incubated with anti-CDK2 monoclonal antibody (1:100) and anti-SCP1 polyclonal antibody (NB300-229, Novus, 1:100) or anti-RAD51 polyclonal antibody (pc130, Millipore, 1:100) and anti-SYCP-3 monoclonal antibody (sc-74569 ...
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bioRxiv - Neuroscience 2023Quote: ... 4-OHT was dissolved in 100% ethanol and then mixed 1:1 with a mixture of 1 part castor oil (Sigma-Aldrich 259853) and 4 parts sunflower oil (Sigma-Aldrich S5007) ...
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bioRxiv - Neuroscience 2022Quote: ... 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM sodium pervanadate, and protease inhibitor cocktail [1:100, Sigma, catalog #P8340]) at 4°C for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: Evolution under antibiotic exposure started with a 1:1 ratio of overnight susceptible and resistant strains diluted 1:50 into fresh media supplemented with ampicillin (Millipore cat# 171254) and lysine (as indicated in the text) ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Genetics 2024Quote: ... The primary antibodies (NF-kB, #8242 Cell Signaling, 1:1000; CD3, MCA-1477 Biorad, 1:100; acetylated Tubulin, T6793 Sigma, 1:1000; Slc12a3, HPA028748, Sigma Aldrich, 1:500 ...
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bioRxiv - Biophysics 2024Quote: ... Panel balance and [Nucleosome : bead region] identity were confirmed using anti-dsDNA (EMD Millipore #MAB030; 1/5, 1/50 and 1/500), anti-H3.1/2 (Active Motif #61629 ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting collagen solution was then mixed in a 1:1 ratio with 1 U/mL bovine thrombin solution (Sigma, Cat. # T4648) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3 times with PBS and incubated with blocking solution (PBS/BSA 1%) for 1 h at 37 °C and then with the following primary antibodies: mouse anti-GFAP (1:500; Millipore, Cat. MAB360), rabbit anti-C3 (1:25 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed and incubated with appropriate secondary antibody diluted in TBS-T (HRP conjugated αMouse, 1:10000, CST, 7076S; HRP conjugated αRabbit, 1:10000, CST, 7074S; HRP conjugated αSheep, 1:5000, Sigma-Aldrich, A3415) for at least an hour before being washed and revealed (BioRad Clarity or Clarity Max ECL) ...
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bioRxiv - Bioengineering 2023Quote: ... Media was then swapped to 200 ng ml-1 FGF9 (R&D, 273-F9-025) and 1 µg ml-1 heparin (Sigma Aldrich, H4784) in TeSR-E6 for 5 days to induce nephron progenitors ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253; mouse anti-β-actin, 1:10.000, Sigma Aldrich) was performed overnight at 4 °C in 5% Albumin Bovine Fraction V (BSA)/TBS-Tween ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with rabbit-anti-Iba-1 primary antibody (1:1000, Wako Chemicals; Cat. No) and WFA (1:400; Sigma; Cat. No) for 23 hours at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Active GLP-1 release in STC-1 cell supernatant was detected with a GLP-1 (Active) ELISA kit (Millipore, Cat#: EGLP-35K). Samples were diluted 10-fold into kit-provided Assay Buffer prior to adding 200 μl sample/well ...
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bioRxiv - Cancer Biology 2024Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3K9me3 antibody (1:500, Millipore, Cat#07-442) or H3K27me3 antibody (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/mL interleukin-4 (IL-4, Sigma-Aldrich) and 0.5 μg/mL anti-CD180 (BD PharMingen) ...
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bioRxiv - Cell Biology 2020Quote: ... 100 nM 4-hydroxy Tamoxifen (4 OT, Sigma, H7904) for 7 days was added on budding large organoids.
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bioRxiv - Neuroscience 2022Quote: ... and fixed with 4% PFA / 4% sucrose (Sigma-Aldrich) for 15 minutes ...