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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Biophysics 2024Quote: Horse Myoglobin protein was purchased from Sigma Aldrich (M1882) and 22.6 mg was resuspended with 700 µL of NMR buffer (20 mM MES pH 6.2 buffer ...
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bioRxiv - Cancer Biology 2024Quote: Proteins were first reduced with 10 mM dithiothreitol (Sigma) for 1 hour at 56°C and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Sigma) and non-specific binding was blocked with 5% milk powder or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured with Bradford reagent (Sigma-Aldrich), and 5 mg of the worm lysate was added to anti-Rabbit IgG magnetic Dynabeads (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra filters (Millipore) to a concentration of 60 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on polyvinylidene (PVDF) (Sigma #P2938) or nitrocellulose (Amersham ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Immobilon FL PVDF membranes (Millipore), and membranes were blocked using Intercept Blocking buffer (Li-Cor) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-myelin basic protein (MBP, Millipore, MAB386, RRID:AB_94975), rabbit anti-laminin (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were developed and detected by chemiluminescence (Millipore) on a ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Cell Biology 2024Quote: Total cell protein was extracted using RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and one Roche Complete protein inhibitor tablet (Millipore, 11873580001). Cells were then incubated with activated concanavalin-A beads (Bang Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were transferred to PVDF membranes (Merck Millipore), and Ponceau S (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: N-terminally His6-Smt3-tagged proteins (pRSFDuet-1, Novagen) were expressed in Rosetta (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using the Bradford assay (Sigma), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and protein G-sepharose CL-4B beads (Sigma-Aldrich). After SDS-PAGE and immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were blotted onto Immobilon-FL PVDF membranes (Millipore), which was subsequently blocked for 1 hour at room temperature using a blocking buffer consisting of 1 part Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated with Amicon Ultra-15 (Millipore, USA) to a final concentration 9 mg/ml.
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bioRxiv - Plant Biology 2024Quote: ... Protein quantity was measured using Bradford method (Sigma-Aldrich). Proteins were stored in aliquots at -80°C until use.
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bioRxiv - Cell Biology 2024Quote: Protein extracts were prepared in RIPA buffer (R0278, Sigma) with protease inhibitor 1X (ab270055 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes (Millipore) using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were wet transferred onto a PVDF membrane (Millipore) and assessed for successful transfer with Ponceau Red (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein expression was induced with 0.2 mM IPTG (Sigma) final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were mixed with DTT (#D9779-10G, Sigma) and loading sample buffer (#NP0007 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membranes (#IEVH85R, Millipore, USA) using the wet transfer system (#1703930 ...
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bioRxiv - Immunology 2024Quote: ... A protein A-Sepharose column (Sigma #ge17-1279-03) was used for mAb purification ...
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bioRxiv - Microbiology 2024Quote: ... We also employed SYPRO Orange Protein stain (Millipore Sigma), a protein dye ...
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bioRxiv - Cancer Biology 2021Quote: ... stained with a 1:40 dilution of anti-human IgG-FITC (Sigma, Mendota Heights, MN) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2021Quote: ... An additional plate was coated with goat anti-human IgG (Sigma, cat. no. I2136-1ML) at 4 μg/ml and IgM (Sigma ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Cell Biology 2020Quote: Human HeLa and HEK293T cell lines (obtained from ATCC) were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (heat-inactivated ...
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bioRxiv - Molecular Biology 2021Quote: ... 2Dorganoids were seeded in chambers pre-coated with 50 μg/ml human collagen IV (Millipore) for 2 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170, Sigma-Aldrich, USA) respectively ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) in 1X PBS 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoblot was then incubated with HRP conjugated-goat anti-human IgG secondary antibodies (Millipore, AP309P) in a dilution of 1:10,000 for 1 h ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for human samples with PAN-Cytokeratin-PE (Sigma-Aldrich, Cat. No. SAB4700668), Vimentin-Alexa Fluor 674 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: Human Embryonic 293T (HEK293T) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2019Quote: HeLa human epithelial cells were recovered from freezer stocks in 10% dimethyl sulfoxide (DMSO, Sigma) and full growth media consisting of Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... blocked with 100 μl/well of 1% human serum albumin (HAS) in PBST (Sigma-Aldrich) for 1 h at RT and then washed 4 times with 100 μl/well of PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
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bioRxiv - Developmental Biology 2019Quote: ... mice received a second IP injection with 5 units of human chorionic gonadotropin (HCG) (Sigma). Immediately after injection females were placed into the cages of stud males ...
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bioRxiv - Cancer Biology 2020Quote: ... SCC-9 and SCC-4 (human tongue OSCC) cell lines were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...