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Citations for Millipore Sigma :
3251 - 3300 of 4323 citations for Recombinant Human IL12B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T cells (Human embryonic kidney, Lehner laboratory stocks) were cultured in Iscove’s Modified Dulbecco’s Media (Sigma) supplemented with 1% GlutaMAX ...
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bioRxiv - Immunology 2020Quote: ... RPMI1640 + 2.05 mM L-Glutamine (Fisher) was supplemented with 10% heat inactivated human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Cells obtained from tumor and ascites were cryopreserved in 50% heat inactivated human AB serum (Sigma), 40% RPMI-1640 (Fisher ...
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bioRxiv - Microbiology 2021Quote: The human embryonic kidney 293T cells (HEK293T) were maintained in Iscove’s modified Dulbecco’s medium (IMDM; Sigma), human THP-1 cells were maintained in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Biochemistry 2021Quote: ... cDNAs for human OCT4 and SOX2 were cloned into the pFLAG-CMV5a mammalian expression vector (Sigma).
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bioRxiv - Bioengineering 2020Quote: ... To the solution was added pre-warmed human cathepsin B (20 ng µL−1, EMD Millipore) in 30 µL MES buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed with PBS and media replaced with human adipocyte differentiation media (Sigma Aldrich). The medium was replaced with fresh human adipocyte differentiation media every 3 days as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Physiology 2019Quote: ... mice were fasted for 5 h before human insulin (0.75 units/kg body weight, Sigma Aldrich) was injected intraperitoneally ...
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bioRxiv - Microbiology 2019Quote: ... Human neutrophils were separated from peripheral blood by density gradient centrifugation using Histopaque (Sigma–Aldrich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal anti-β-actin (mouse anti-human primary, CAT# A2228) antibody was purchased from Sigma-Aldrich. Proteome Profiler Human Apoptosis Array kit (CAT# ARY009 ...
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bioRxiv - Physiology 2021Quote: ... Insulin concentrations after the 16 h hyperinsulinemia treatment were determined using human insulin RIA kit (Millipore). To mimic starvation ...
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bioRxiv - Genetics 2020Quote: ... Caveolin knockdown was achieved by transfection with siRNA against caveolin (MISSION® esiRNA human CAV1, Sigma) or negative control siRNA (MISSION® siRNA Universal Negative Control #1 ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the blots were incubated with 1:20000 HRP-conjugated rabbit anti-human IgG (Sigma-Aldrich, #A8792). For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The shRNA lentiviral plasmids (pLKO.1-puro) for human/mouse/rat frataxin were purchased from Sigma. The RefSeq used was NM-008044 ...
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bioRxiv - Cell Biology 2019Quote: ... with human cardiac stem cell medium (hCSC-medium) consisting of DMEM/F-12 medium (Sigma Aldrich), basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2020Quote: ... 30×106 PBMCs from healthy human donors were lysed in isotonic buffer containing 1% Brij58 (Sigma) on ice for 30 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... Schneider’s Insect Media (10% FBS, 1% pen/strep) supplemented with 200 mg/ml Human insulin (Sigma) was added and ovarioles were maintained at room temperature for 2-3 hours.
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bioRxiv - Cell Biology 2021Quote: ... pre-coated with human type IV collagen (catalog number C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day-2) ...
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bioRxiv - Biochemistry 2021Quote: ... PMSG-primed females were injected with 500 IU of human chorionic gonadotropin (hCG, Sigma-Aldrich, CG10). Freshly laid eggs were collected and chemically dejellied in 2% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... human polyclonal IgG and molecular biology grade water were purchased from Sigma-Aldrich (St. Louis, MO). Deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Developmental Biology 2021Quote: ... followed 46–48 h later by 5 IU human chorionic gonadotropin (Sigma Aldrich, St. Louis, MO). The superovulated females were also bred overnight to vasectomized males for consistency across both treatment groups ...
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bioRxiv - Microbiology 2021Quote: pLEX-IMPDH2-V5 vector was picked from the MISSION TRC3 human LentiORF library from Sigma-Aldrich. The pcDNA-FLAG-V5-Nsp10/14/16 vectors were constructed from pDONR223 SARS-CoV-2 Nsp10 (Cat ...
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bioRxiv - Microbiology 2020Quote: ... 1:50 polyclonal antibody against full-length human C1QBP (α-HsC1QBP) derived from mouse (Sigma-Aldrich), or both overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... human colorectal adenocarcinoma cell line Caco-2 were maintained in Dulbecco’s Modified Eagle’s Media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
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bioRxiv - Immunology 2021Quote: shRNA clones were obtained from the whole RNAi human library for shRNA mediating silencing (Sigma, Aldrich) maintained at IISER ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-RBFOX1 (1:2000 for mouse and 1:1000 for human samples, Merk Millipore, MABE985), mouse anti-RBFOX2 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Human alveolar epithelial cells (A549) and green monkey epithelial cells (Vero) were cultured in DMEM (Sigma) containing 10% FCS ...
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bioRxiv - Neuroscience 2022Quote: ... Transfections were carried out using 30 pmols of MISSION esiRNA targeting human FAAH (EHU098921; Sigma Aldrich) or a universal negative control (SIC001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Miltenyi) and expanded for 14 days in RPMI supplemented with 10% human AB serum (Sigma-Aldrich), 6000 IU/ml human IL2 (Peprotech) ...
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bioRxiv - Cell Biology 2022Quote: Human neuroblastoma SH-SY5Y cells (ATCC CRL-2266) were maintained in DMEM/F-12 (Sigma-Aldrich) as described in Silveyra et al ...
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bioRxiv - Immunology 2022Quote: ... followed by staining with mouse IgG2a anti-human α- Smooth muscle actin (SMA; clone 1A4; Sigma) and mouse IgG1 anti-Collagen 1 (clone Col1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolation of PBMCs from human blood was performed by density gradient centrifugation on Histopaque-1077(Sigma). Briefly ...
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bioRxiv - Physiology 2022Quote: ... Anti-human T1R3 SAB4503300 (rabbit polyclonal) and acetylated tubulin T7451 (mouse monoclonal) antibodies were from Sigma. Anti-human T1R3 ab150525 (rabbit polyclonal ...
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bioRxiv - Pathology 2024Quote: ... whereas human LDL were obtained commercially and labeled with Cy3 (Millipore Sigma Canada Ltd, ON, Canada). Previous studies have demonstrated that ApoB48-Cy5 and ApoB100-Cy3 are not transferred between lipoproteins and do not have altered clearance from plasma ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Cell Biology 2024Quote: Human cells (HeLaK and Hek293T) were cultured in DMEM (Biowest) supplemented with 10% FCS (Sigma-Aldrich) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... trans-well membranes were coated with human type IV collagen (Sigma-Aldrich, St. Louis, MO, USA) and then rinsed with 1X phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Mass spectrometer calibration was performed with a [Glu1]-fibrinopeptide B human (Glu-Fib) (Sigma-Aldrich, USA) solution with an MS/MS spectrum reference from the NanoLock Spray source ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Immunology 2024Quote: ... and then incubated with the primary antibody (rabbit anti-human lactoferrin antibody; Sigma, Cat-No. L3262) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... falciparum parasites were cultured in vitro in ORh+ human erythrocytes in RPMI 1640 (cat # R8758; Sigma) supplemented with 10% heat inactivated human serum and 5 mg/L gentamycin at 37°C in 5% O2 and 5% CO2 in N2 ...
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bioRxiv - Microbiology 2023Quote: ... the MICs were also determined in presence of 4% (w/v) human serum albumin (Sigma # A1653) which is defined here as the serum shifted MIC.
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bioRxiv - Molecular Biology 2023Quote: ... sequences flanking the Human TRR-TCT3-2 gene are in italics) was ordered from Sigma Aldrich. Non-template and template strands were annealed in H2O followed by heating to 95 ⁰C for 5 min before cooling down to 20 ⁰C at a rate of 1 ⁰C/min ...
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bioRxiv - Physiology 2023Quote: Immortalized human HepG2 cells (ATCC, Manassas, VA, USA) were cultured in phenol-free DMEM (Sigma-Aldrich) containing 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Human VSMCs were isolated as previously described90 and were cultured in Dulbecco’s modified Eagle medium (Sigma) supplemented with 20% fetal calf serum ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by single dose 120 mg/kg Abraxane (Royal Marsden Hospital) or human albumin control (Sigma) the next day ...