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Citations for Millipore Sigma :
3251 - 3300 of 4463 citations for Recombinant Human IL12B Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... HEK293 cells were cultivated in DMEM with Na-pyruvate (BIOCHROME, Germany) supplemented with 10 % fetal calf serum (FCS) and 4 mM L-Glutamine (Sigma Aldrich GmbH St Louis, MO, USA) at 7% CO2 and 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Cancer Biology 2021Quote: ... stained with a 1:40 dilution of anti-human IgG-FITC (Sigma, Mendota Heights, MN) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2021Quote: ... An additional plate was coated with goat anti-human IgG (Sigma, cat. no. I2136-1ML) at 4 μg/ml and IgM (Sigma ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Cell Biology 2020Quote: Human HeLa and HEK293T cell lines (obtained from ATCC) were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (heat-inactivated ...
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bioRxiv - Molecular Biology 2021Quote: ... 2Dorganoids were seeded in chambers pre-coated with 50 μg/ml human collagen IV (Millipore) for 2 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170, Sigma-Aldrich, USA) respectively ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) in 1X PBS 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoblot was then incubated with HRP conjugated-goat anti-human IgG secondary antibodies (Millipore, AP309P) in a dilution of 1:10,000 for 1 h ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for human samples with PAN-Cytokeratin-PE (Sigma-Aldrich, Cat. No. SAB4700668), Vimentin-Alexa Fluor 674 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: Human Embryonic 293T (HEK293T) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2019Quote: HeLa human epithelial cells were recovered from freezer stocks in 10% dimethyl sulfoxide (DMSO, Sigma) and full growth media consisting of Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... blocked with 100 μl/well of 1% human serum albumin (HAS) in PBST (Sigma-Aldrich) for 1 h at RT and then washed 4 times with 100 μl/well of PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
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bioRxiv - Developmental Biology 2019Quote: ... mice received a second IP injection with 5 units of human chorionic gonadotropin (HCG) (Sigma). Immediately after injection females were placed into the cages of stud males ...
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bioRxiv - Cancer Biology 2020Quote: ... SCC-9 and SCC-4 (human tongue OSCC) cell lines were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using goat-anti-human-IgM-HRP (1:1000) (Sigma catalogue no. A6907) and wells were washed with PBS-T and one-step 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2021Quote: ... (3) TexMACS™ medium (Miltenyi) was supplemented with 3% heat-inactivated human AB serum (Sigma) and 1X Penicillin Streptomycin solution (TAC) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Pre-clinical Repository) ...
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bioRxiv - Bioengineering 2021Quote: ... Fluorescein isothiocynate human serum albumin (FITC-HSA) was purchased from Sigma Aldrich (Oakville, ON, Canada). Milrinone was purchased from Selleck Chemicals (Burlington ...
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bioRxiv - Neuroscience 2020Quote: ... the frogs previously injected with PMSG were injected with human chorionic gonadotropin (hCG, 400UI, Sigma) to induce egg laying ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with pLKO.1 shRNA plasmid (Sigma, Mission human genome shRNA library) and packaging plasmids-delta 8.9 ...
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bioRxiv - Cancer Biology 2020Quote: ... human cells iso-lated and treated in vitro with 50nM TAM (Sigma-Aldrich, H7904-25G) to induce recombination and shRNA expression ...
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bioRxiv - Cell Biology 2021Quote: ... 32 human Nups were systematically depleted using two different commercially available siRNA pools (Dharmacon; Sigma). siRNAs were transfected into the HeLa cells at a 50 nM final concentration using RNAiMAX (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in AIM V (Thermo) supplemented with 5% human serum (Sigma #H3667). PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Systems Biology 2020Quote: ... 35mm glass Mat-tek dishes were coated with 10µg/ml Fibronectin (human plasma, Sigma #F0895) for 1 hour ...
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bioRxiv - Physiology 2021Quote: Plasma FGF21 (pg/ml) was measured using a human FGF21 ELISA kit (Millipore, Billerica, MA). In Experiment 2 ...
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bioRxiv - Microbiology 2021Quote: ... a human IgG1 kappa standard purified from myeloma plasma (in duplicates per plate, Sigma-Aldrich) and serum samples (starting at a 1:20 dilution ...
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bioRxiv - Cancer Biology 2020Quote: HCT116 human colon adenocarcinoma cell lines were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma). Cell culture media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2021Quote: ... 2×106 cells/ml sperm were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Human PBMC were isolated from the peripheral blood of healthy volunteers using Ficoll (Sigma-Aldrich) density centrifugation in Greiner LeucoSep-tubes (Sigma ...
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bioRxiv - Genetics 2022Quote: ... the corresponding cDNA fragment of human FLCN was cloned in the pET-30 vector (Novagen) and the His-tagged fusion protein was expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... The COCs were incubated in human tubal fluid (HTF) medium (Millipore Corp; MR-070-D) with appropriate concentration of sperm obtained from 1 hour capacitation ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX, C3657, Sigma-Aldrich, St Louis, MO) was pre-solubilized in aqueous acid and diluted in carbonate coating buffer ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Bioengineering 2019Quote: Genotoxicity assay was determined using Hek 293T cells (Human embryonal kidney cells, Sigma-Aldrich, USA) according to Comet assay as previously described [31] ...
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bioRxiv - Cell Biology 2020Quote: ... pre-coated with human type IV collagen (Cat# C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day −2) ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Microbiology 2020Quote: Human foreskin fibroblasts (Hff1; ATCC® SCRC-1041™) were maintained in DMEM (Sigma-Aldrich) supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin and 100 µg/mL streptomycin.
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lines and primary human fibroblasts (MRC5) were cultured in DMEM (D6546, Sigma-Aldrich) with 2 mM L-glutamine ...