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Citations for Millipore Sigma :
3251 - 3300 of 3774 citations for Gastric triacylglycerol lipase LIPF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: The human multiple myeloma cell line (MMCLs) MM.1S (ATCC, Manassas, VA, USA) was cultured in RPMI1640 media (Sigma, UK) and 20% FBS (Life Technologies) ...
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bioRxiv - Developmental Biology 2020Quote: ... Metaphase II-arrested oocytes tightly packed with cumulus cells were collected from the oviductal ampulla 14 h after hCG injection and placed in a 100-μl drop of human tubal fluid (HTF; Millipore) medium covered with mineral oil ...
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bioRxiv - Cell Biology 2020Quote: ... A2780 human ovarian cancer cells (female) stably expressing Rab25 (Caswell et al., 2007) were maintained in RPMI-1640 medium (Sigma) supplemented with 10 % FCS ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Immunology 2020Quote: ... Freshly-isolated neutrophils were suspended at 106 or 5×106 cells/mL in RPMI media (containing 10% human AB serum (Sigma)) and incubated at 37°C and 5% CO2 for up to 18h ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: Human HeLa229 cells (ATCC CCL-2.1tm) and human epithelial conjunctival cells (Chang) were cultured in 10 % (v/v) heat inactivated FBS (Sigma-Aldrich) RPMI1640 + GlutaMAXtm medium (Gibcotm ...
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bioRxiv - Immunology 2020Quote: ... the protein transferred membranes were blocked with 5% dry milk in PBST (PBS with 0.05% tween-20) overnight at 4°C and probed with goat anti-human IgG-HRP (Sigma-Aldrich, St ...
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were thawed and plated at 106 cells per cm2 in 20 ml RAB5 (RPMI with 5% human serum AB (Sigma), 2 mM L-alanyl-L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Parasite cultures were maintained in a sus-pension of human erythrocytes at 2% hematocrit in complete media (RPMI-1640, Millipore-Sigma, supplemented with 27mM sodium biocarbonate ...
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bioRxiv - Microbiology 2021Quote: ... Parasite cultures were maintained in a sus-pension of human erythrocytes at 2% hematocrit in complete media (RPMI-1640, Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from human hippocampal and prefrontal cortex tissue and mouse hippocampal tissue was extracted with TRIzol reagent (Sigma-Aldrich) and 1 μg was reverse-transcribed using PrimeScriptTM RT Reagent Kit (perfect Real Time ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... The slides containing hamster and human lung tissue sections were deparaffinized in xylene (Sigma-Aldrich Inc., MO, USA; catalog# 534056) and rehydrated in graded alcohols to water ...
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bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
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bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
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bioRxiv - Cancer Biology 2022Quote: ... and subcultured for 72 h in NK MACS medium supplemented with 5% human serum male AB plasma (#H4522, Sigma-Aldrich) and human IL-2 IS (200 IU/ml ...
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bioRxiv - Biophysics 2022Quote: ... Cells were counted and further diluted at the required concentration for the following procedures in RPMI 1640 containing 10mM HEPES (Roth) and 0.5% human serum albumin (Sigma Aldrich). Purity of the isolation was assessed by a cytospin assay (Cytospin 2 Zentrifuge ...
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bioRxiv - Cell Biology 2022Quote: Human monocyte-derived macrophages were separated from healthy donor buffy coats by centrifugation through Histopaque 1077 (Sigma-Aldrich, Cat # H8889) gradient ...
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bioRxiv - Microbiology 2022Quote: Total protein was extracted from LT toxin-treated (100ng/ml) human differentiated enteroid monolayers (235D line) and untreated control using radioimmunoprecipitation assay (RIPA) buffer (Sigma) containing protease inhibitor cocktail ...
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bioRxiv - Microbiology 2022Quote: ... The infectious blood meal consisted of a 2:1 mix of washed human erythrocytes and viral suspension supplemented with 10 mM ATP (Sigma). The infectious titers were 107 FFU/mL for DENV-1 ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of mouse TRAIL (aa118-291) or human TRAIL (aa115-281) was amplified from its cDNA and was cloned into pET21b (Novagen) using NdeI and XhoI sites ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... the HepG2 human hepatocyte carcinoma cell line and the HT29 human colon adenocarcinoma cell lines were purchased from the European Collection of Authenticated Cell Cultures (ECACC) through their distributor (Sigma). The HL1 cell line was purchased from Millipore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Molecular Biology 2024Quote: ... Follicles were then cultured in αMEM-based maturation media supplemented with 10% FBS and 1.5 IU/mL Human chorionic gonadotropin (hCG, Sigma-Aldrich) in a an incubator (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Cell Biology 2023Quote: ... Rescue experiments were performed by supplementing the medium with either 25 µg/mL human plasma fibronectin (Millipore Sigma; Catalogue #FC010) or with 10 ng/mL of human TGFβ1 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-Flag affinity matrix (catalog no. A2220) and albumin from human serum (HSA, Cat No. A3782) was purchased from Sigma. The FITC Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... Antibody for β-actin (Catalog no. A2228) and human α-thrombin (Catalog no. T6884-100N) was purchased from Sigma-Aldrich. All other reagents were bought from Fisher-Scientific except where noted.
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bioRxiv - Microbiology 2024Quote: ... 10 ng recombinant IdeS was pre-incubated with sera dilutions as indicated at 37°C 1 h before addition of 100 ng human IgG (Sigma) in assay diluent (AD ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RH ΔKU80Δhxgprt AtTIR1 and RH TatiΔKU80 strains were grown in Human Foreskin Fibroblasts (HFF) (ATCC, SCRC-1041) in DMEM media (Millipore Sigma) supplemented with 10% FBS [56 ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... a spectrophotometric stop reaction was used to calculate the activity of human salivary α-amylase (Sigma-Aldrich, cat. No. 1031) using Equation 1 (b = intercept of linear regression ...
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bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... Four- to five-week-old C57Bl/6 females were superovulated and MII oocytes collected in fertilisation media (Human Tubal Fluid (HTF, MR-070, Sigma) containing 4 mg/ml BSA and 25mM L-Glutathione 1 (GSH)) ...
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bioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gels were then immediatly coupled to either human plasma fibronectin (1 mg/mL overnight at 37◦C; FC010, Millipore) or ICAM-1 (0.1 mg/mL Recombinant Protein A (Novex ...
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bioRxiv - Immunology 2023Quote: ... DH270 UCA and anti-c-myc antibody binding were detected with 500 µl of 10 µg/ml goat-anti-human PE (Sigma) and 10 µg/ml goat anti-chicken Alexa 647 respectively ...
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bioRxiv - Biophysics 2024Quote: The tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...