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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The FLAG-tagged WT sCTLA-4 or Y139A sCTLA-4 in the culture supernatant was purified with the anti-FLAG M2 affinity gel (Sigma-Aldrich), washed with wash buffer (Phosphate buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... Newly synthesized RNA was then labelled by incubating the cells for 1h with 100 µM 4-thiouridine (4-sU, Sigma-Aldrich). RNA extraction was performed with peqGOLD TriFast Reagent (VWR ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Biophysics 2021Quote: ... 10 units of Glucose-6-phosphate Dehydrogenase from baker’s yeast (G6PDH; S. cerevisiae; Sigma-Aldrich) and either 0 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected after 48 h using 6 µg/ml Blasticidine S (Sigma Aldrich) for 2 weeks.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 10 μM 6-Diazo-5-oxo-L-norleucine (DON, Sigma Aldrich), 1 μM Palbociclib (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... cdc12-6 cultures were shifted to 37°C and treated with 200 mM hydroxyurea (Sigma) for 1 h to protect cells from subsequent phototoxicity during imaging (Howell et al. ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Genetics 2020Quote: ... 3 mg of each compound was dissolved in 6 mL hexane (Sigma-Aldrich #139386-500ML) and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab ...
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bioRxiv - Molecular Biology 2021Quote: ... IL-6 and TNFα using Milliplex Map Mouse Serum Adipokine Panel (Millipore catalogue # MADKMAG-71K) on a Luminex 200IS platform.
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bioRxiv - Genomics 2020Quote: ... 20000 cells were directly lysed in 6 μL of 0.17% TrionX-100 (T8787, Sigma-Aldrich) for 3 min at 72 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Newly synthesized (ns) RNAs were labelled for 6 min with 0.4-thiouracil (4tU, Sigma-Aldrich) added to the yeast cultures to a final concentration of 5 mM ...
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bioRxiv - Cell Biology 2019Quote: ... Hindlimb muscles from 6 days pups were extracted and digested with collagenase (Sigma, C9263-1G) and dispase (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... and plated onto 6 well cell culture dishes coated with Poly-L-Ornithine (Sigma P2533) at a density of 1 million cells per well.
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bioRxiv - Developmental Biology 2019Quote: ... Tissue was suspended in cold methanol with 1 mM 6-propyl-2-thiouracil (PTU; Sigma) and homogenized by sonication ...
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bioRxiv - Evolutionary Biology 2019Quote: ... larvae were incubated with anti-acetylated α-tubulin primary antibody (clone 6-11B-1, Sigma) and AlexaFluor-488 secondary antibody (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... except 6-AM which was dissolved in 1% lactic acid (Sigma Aldrich, St. Louis, MO). Drugs were administered SC or IV at an injection volume of 1-2 ml/kg and all drug doses were expressed as the salt forms listed except for 6-AM which was expressed as the base form ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... Laurdan reagent (6-dodecanoyl-N,N-dimethyl-2-naphthylamine) was purchased from Sigma-Aldrich (Merck) and dissolved in N,N-dimethylformamide (DMF) ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... 30 µL of CSF was incubated with 0.03% CHAPS with 6 M urea (Sigma U0631) and 20 mM TCEP (Pierce 77720 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Unstimulated cells were kept in the dark for 6 h before addition of resazurin (Sigma) at a final concentration of 55 μM ...
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bioRxiv - Physiology 2021Quote: ... cardiac perfusion was performed with 6 mL of cycloheximide (100 μg/ml in PBS, Sigma). Harvested tissues were immediately placed in a lysis buffer consisting of 1% Triton X-100 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were grown in the presence of 10 µM 6-thioguanine (Sigma-Aldrich, A4882-250MG) for 6 days ...
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bioRxiv - Plant Biology 2021Quote: ... Colonies were inoculated in LB-media containing carbenicillin (Sigma Aldrich; cat No. 4800-94-6), 0.2 % lactose (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Quantification of the staining was performed by dissolving the micromasses with 6 M guanidine (Sigma) for 6h and measuring the absorbance at 595 nm with a spectrophotometer (BioTek Synergy).
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bioRxiv - Developmental Biology 2020Quote: ... 5 mM EDTA) supplemented with 50 μg/ml Proteinase K (Sigma-Aldrich, 39450-01-6) and reverse-crosslinked at 68°C for 2 hrs with vigorous shaking at 14,000 rpm in a thermal mixer ...
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bioRxiv - Biophysics 2021Quote: ... and 1 mM (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; 238813-5G, Sigma-Aldrich). To obtain images for training the DeepSTORM model ...
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bioRxiv - Cancer Biology 2020Quote: ... and treated with nigericin (10 μM, 6 h), or ATP (5 mM, 30 min) (SIGMA). IL-1β ELISA was purchased from BD ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was then concentrated to 3-6 mg/ml (Amicon 100 kDa cutoff, Millipore) and loaded onto a size-exclusion column equilibrated with 20 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... after conversion of glucose to gluconate-6-phosphate (hexokinase; Sigma-Aldrich, St. Louis, MO, USA). An aliquot of the original extract was treated with invertase and phosphoglucose-isomerase (both Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) was obtained from Sigma-Aldrich (Cat No. C127). Tetrodotoxin (TTX ...
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bioRxiv - Cell Biology 2022Quote: ... which were washed 5-6 times in ice-cold PBS with 1.5 mM DTT (Sigma). Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... We then prepared a solution of 10 mg/ml 6-OHDA (H116, Sigma-Aldrich, MO) dissolved in 0.2% ascorbic acid (1043003 ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC colonies were passaged weekly onto 6-well plates coated with 0.1% gelatin (EMD Millipore) and pre-seeded with a feeder layer of irradiated mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Genetics 2022Quote: ... 3 mg of each compound was dissolved in 6 mL hexane (Sigma-Aldrich #139386-500ML) and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab ...
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bioRxiv - Bioengineering 2022Quote: ... 19 combinations of buffer (6 M urea [Sigma] in TBS [Fisher], pH 9.5; TBS; PBS) temperature (50°C ...
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bioRxiv - Developmental Biology 2022Quote: ... inflorescences from 3-week-old plants were treated with 5 μM 6-BA (Sigma-Aldrich), 50 μM IAA (Sigma-Aldrich) ...