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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Microbiology 2019Quote: ... The re-suspensions were spread on 7H11 OADC plates supplemented with the relevant drug concentration (2, 4, or 8 µg/mL of ofloxacin, or 100 µg/mL streptomycin; Sigma). Resistant colonies were observed and enumerated after 21 to 35 days of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29, #Millipore) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Microbiology 2019Quote: ... and media with 4 mM Ca2+ was made by adding 2 mM CaCl2 to the standard high-glucose DMEM (Sigma). We maintained TV-infected cells under these conditions until the positive control (normal media ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total protein from PC12 cell extracts were incubated over night at 4°C with 2 μg of the phospho- tyrosine 4G10 (Sigma-Alrich/Upstate Cat# 05-321 ...
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bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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Physical activity increases neuronal activity in the circadian clock of diurnal Arvicanthis ansorgeibioRxiv - Neuroscience 2024Quote: ... The brain was then removed and fixed for 2 days in 4% paraformaldehyde containing C6FeK4N6·3H20 (Sigma-Aldrich, Lyon, France). Coronal slices (40 µm thick ...
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bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Molecular Biology 2023Quote: The feeder-dependent murine ESC line v6.5 and feeder free Bruce-4 cells were cultured in 2% gelatin-coated (SIGMA, G1393) ventilated-cap flasks ...
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bioRxiv - Developmental Biology 2023Quote: We scanned formalin-fixed whole kidneys stained for 2 hours in 4% OsO4 solution (Sigma-Aldrich, St. Louis, MO, USA) using a previously developed method 31 ...
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PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich, cat.no. 59427C-100ML). HEK293T-LentiX (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified by centrifugation and incubated for 2 hours at 4 °C with anti-FLAG M2 agarose beads (Sigma) with gentle rotation ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... flies were homogenized in 50 μl PBS per fly (between 2-4 flies per sample) containing 0.1% Tween-20 (Sigma #1379) using a TissueLyser LT (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... Next, 50 μl of 2× STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA (cat. E3889, Sigma-Aldrich) and 100 μg/ml RNase A (cat ...
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bioRxiv - Molecular Biology 2024Quote: MM-NLP particles isolated from both sucrose gradient sedimentation (native) and GraFix (crosslinked) were concentrated to 2 µM using Amicon Ultra-4 centrifugal filters (Ultracel 30K, Millipore). C-Flat 1.2/1.3 (Cu ...
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bioRxiv - Microbiology 2021Quote: ... 150 μL of Kovacs reagent (4-dimethylaminobenzaldehyde and hydrochloric acid solution in n-butanol; Sigma Aldrich) was placed into a 96 well clear bottom plate for each sample to be measured ...
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bioRxiv - Cancer Biology 2019Quote: ... 4-Nitroquinoline N-oxide (N8141) and Thapsigargin (T9033) were from Sigma-Aldrich (St. Louis, MO, USA). Etoposide (341205 ...
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bioRxiv - Pathology 2020Quote: ... and 4-methylumbelliferyl N-acetyl b-D-glucosaminide (Sigma Aldrich, Cat. No. M2133, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500 ...
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bioRxiv - Biophysics 2020Quote: ... 5-(N-Ethyl-N-isopropyl)amiloride (EIPA, Sigma-Aldrich, A3085), phenamil (phen ...
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bioRxiv - Developmental Biology 2022Quote: ... a single dose of 1 mg of 4-hydroxytamoxifen (4-OHT, H7904, Sigma, ≥98% Z (trans) isomer ...
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bioRxiv - Genomics 2022Quote: ... 0.01% [w/w] 4-hydroxy- 2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma-Aldrich, cat# 176141), 0.2% [w/w] tetramethylethylenediamine (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of 0.5 nmol α-Cyano-4-hydroxycinnamic acid (4-CIN, Sigma Aldrich, Cat #: C2020) dissolved in ACSF containing DMSO (Wang ...
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bioRxiv - Cell Biology 2022Quote: ... pLKO.1-puro-CMV-tGFP plasmids targeting SNX5 (5’-ACTATTACAATAGGATCAAAG-3’, 5’-CTGAGTATCTCGCTGTGTTTA-3’) and SNX6 (5’-AGTAAAGGATGTAGATGATTT-3’, 5’-GCCGAAACTTCCCAACAATTA-3’) (Sigma-Aldrich) were lentivirally transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Cell Biology 2021Quote: ... 150 μM 4-(4-Morpholinobutylthio)phenol (MoTP) (Sigma) was added to embryos kept at 28.5°C from 24hpf onwards ...
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bioRxiv - Neuroscience 2020Quote: ... 4-AP (4-Aminopyridine, 500 μM, Sigma-Aldrich), FK-506 (1 μM ...
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bioRxiv - Bioengineering 2019Quote: ... (ii) 4 hours of 4% sodium deoxycholate (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma and dissolved in ethanol ...