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Citations for Millipore Sigma :
3201 - 3250 of 3874 citations for Recombinant Human ITCH None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Female frogs (4-15 years old) were injected subcutaneously with 300-700 units of human chorionic gonadotropin (hCG; Sigma), depending on weight and age ...
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bioRxiv - Developmental Biology 2020Quote: ... sperm were collected from the vasa deferentia and then capacitated for 1.5 hours in human tubal fluid (HTF) medium (Millipore). Sperm cells were then incubated with intact cumulus-oocyte complexes or zona-free oocytes for 5 hours ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells (a human embryonic kidney cell line; ATCC CRL-1573) were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) containing fetal calf serum and antibiotics.
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Immunology 2021Quote: ... 0.3 x 106 primary human keratinocytes were seeded directly after transfection in 500 µl keratinocyte medium supplemented with 1.5 mM CaCl2 (SIGMA) into the insert ...
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bioRxiv - Immunology 2020Quote: ... were incubated overnight at 4°C with 50 μL of a solution of in 75 mM sodium carbonate (pH 9.6) containing either: 2.5 μg/ml aggregated human IgG (Sigma-Aldrich) for CP ...
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bioRxiv - Biophysics 2021Quote: Human serum albumin (fraction V) 98% and 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) were purchased from Sigma Aldrich (USA) and used without any prior purification ...
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bioRxiv - Genomics 2020Quote: ... Cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% AB human serum (Sigma Aldrich), 100 U/mL recombinant human IL-2 (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... were co-expressed with the C-terminal domain of human DNMT3L (residues 178–386 of NCBI accession NM_175867) on a modified pRSFDuet-1 vector (Novagen), in which the DNMT3B or DNMT3A sequence was preceded by a hexahistidine (His6 ...
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bioRxiv - Neuroscience 2021Quote: Dissociated human iPSC-derived motor neurons were re-plated on poly-D-lysine (PDL; 100 µg/ml; Sigma-Aldrich) and CSPG-(0.2 ng/ml ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... NHP and human hepatocytes from xenograft FRG mice and NHP tissues was extracted using the TriReagent (Sigma, Cat# T9424)-chloroform protocol previously published.35,36
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bioRxiv - Cell Biology 2022Quote: ... The same ex vivo culture system was used to assess human lung cytokine responses to lipopolysaccharide (LPS, L5668, Sigma) stimulation by ELISA ...
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bioRxiv - Cell Biology 2022Quote: ... The following day the cells were washed with PBS and media changed to human osteoblast differentiation media (Sigma-Aldrich). Osteoblast differentiation media was changed every 3 days for a period of 14 days as per the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Transfer plasmids with pre-designed shRNA against human Cdc42 mRNA were obtained from the Mission® TRC library (Sigma). Specifically ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were assayed for secreted chemokines and cytokines using the Milliplex 38-plex Human Cytokine/Chemokine Assay (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Secondary incubation was performed with 1:20 000 dilution of HRP-conjugated anti-human IgG antibody (Sigma Aldrich SAB701283) for 1 hour at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Media aliquots were frozen and subjected to ENG ELISA analysis with the Human Endoglin ELISA kit (Sigma-Aldrich RAB0171) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: We obtained thirtysix 20 amino acid peptides overlapping by 5 amino acids in 0.5mg amounts in 96 well format microtubes based on the entire human NF-L sequence in NP_006149.2 (Sigma-Aldrich). Peptides were diluted in 50% acetonitrile/50% PBS to a concentration of 2.5mg/mL and 2mL of each peptide was arrayed in a 96 well plate ...
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bioRxiv - Microbiology 2022Quote: ... Cells were fixed and permeabilized with Cytofix/Cytoperm (Beckton Dickinson) and incubated with diluted human γ-globulins (G4386; Sigma) on ice for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Log-phase bacteria were resuspended in RPMI 1640 infection media: supplemented with 10% human serum (Millipore Sigma, Burlington, MA), 10 mM HEPES ...
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bioRxiv - Developmental Biology 2022Quote: ... CA) at least 48 h before use and boosted with 500 IU of HCG (Human Chorionic Gonadotropin CG10, Sigma) 14-16 hours before experiments ...
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bioRxiv - Pathology 2024Quote: ... Primary human hepatocytes were treated with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, 200 ng/ml, Sigma, #T9701) for 6 hours to induce apoptosis ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Pathology 2024Quote: ... This mixture was then pipetted into Mantarray casting wells containing 50µL EHT culture media with 0.3U human thrombin (Sigma-Aldrich). EHTs were then incubated for one hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were cultured in human O+ erythrocytes (deidentified from commercial vendor) at 4% hematocrit (HCT) in RPMI-1640 (Sigma) supplemented with 25 mM HEPES (4-(2-hydroxyethel)-1-piperazineethanesulfonic acid ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Cell Biology 2024Quote: ... The probe was resuspended in a solution containing 38 ng/μL human Cot-1 DNA (Millipore Sigma, Cat # 11581074001), 256 ng/μL yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and AP-labeled goat anti-human IgA (α-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) was used for detection ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (HCT116, human colon cancer cell line, ATCC) were transduced with lentivirus using 8 μg/ml polybrene (Sigma). 72 hours after lentivirus transduction ...
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bioRxiv - Biochemistry 2022Quote: ... residues 353-598) and human RXRα (NR2B1) LBD (residues 223-462) that were inserted into a pET45b(+) plasmid (Novagen) as a TEV-cleavable N-terminal hexahistidine(6xHis)-tag fusion protein ...
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bioRxiv - Cancer Biology 2023Quote: Two individual pre-designed human PLXNB2 sgRNA CRISPR-Sanger clones and one non-targeting control vector (Sigma-Aldrich #0020) were ordered from Sigma as glycerol stock (Sigma-Aldrich #HS5000013567 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were extensively washed in 0.1M PB and TBS and blocked in 1% human serum albumin (HSA; Sigma-Aldrich) solution for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48h later by intraperitoneal injection of 5 IU human chorionic gonadotropin (PMS from SIGMA, HGC from Millipore USA). Mouse zygotes were obtained by mating B6/CBA stud males with superovulated B6/CBA females at a 1:1 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48h later by intraperitoneal injection of 5 IU human chorionic gonadotropin (PMS from SIGMA, HGC from Millipore USA). Mouse zygotes were obtained by mating B6/CBA stud males with superovulated B6/CBA females at a 1:1 ratio ...
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bioRxiv - Molecular Biology 2023Quote: Human induced pluripotent stem cell derived cardiac fibroblasts (iPSC-FBs) were cultured in FibroGRO Complete Medium (SCMF001; Millipore Sigma) with 2% FBS at 37°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphate buffered saline (PBS) containing 0.12% of DMSO and 4% of human serum albumin (HSA) (Sigma-Aldrich, MO, USA) was used as the negative control and incubation buffer ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation using Hisopaque-1077 (Millipore) and SepMate PBMC isolation tubes (StemCell ...
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bioRxiv - Microbiology 2023Quote: ... Human liver hepatocellular carcinoma (HepG2) and Vero (African Green Monkey Kidney) cells (ATCC) were regularly maintained in DMEM (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... we added a calibration range by serially diluting a polyclonal IgG antibody from human sera solution (reference: I2511, Sigma), to reach concentrations ranging from 0.68 ng.mL-1 to 22 ng.mL-1 ...
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bioRxiv - Immunology 2023Quote: Human Vγ9Vδ2 T cells were specifically expanded from fresh PBMCs ex vivo using 5 µM zoledronic acid (Sigma Aldrich) or 3 µM BrHPP (bromohydrin pyrophosphate ...
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bioRxiv - Cell Biology 2023Quote: ... human breast cancer (MCF7) and human embryonic kidney cells (HEK293T) cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...