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Citations for Millipore Sigma :
3201 - 3250 of 4323 citations for Recombinant Human CD5L His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 µg/mL, HPA006028, Sigma/Prestige Antibodies) was performed at 4 °C overnight in PBS/10% AB serum ...
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bioRxiv - Neuroscience 2024Quote: ... sections were double-immunolabeled for human nuclei and either Htt (1:200; clone EM48, Millipore) or IBA-1 (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... which were coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO; C7521). Ex Plus medium was added to both the apical and basolateral compartments and changed daily until the cells reached confluency on the inserts ...
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bioRxiv - Microbiology 2024Quote: ... TSB-grown bacteria were centrifuged and resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 °C with shaking (180 rpm) ...
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bioRxiv - Biochemistry 2024Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
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bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... we coated plates with 1 µg/ml of recombinant NP119 and used a goat anti-mouse IgG-HRP (ψ-chain specific) as secondary antibody (Sigma, A3673-1ML; diluted 1:1000). To measure anti-drug antibody responses directed against the AAV-delivered YTS169-IgG2a we coated plates with 1 µg/ml of YTS169-IgG2a or YTS191-IgG2a and used a goat anti-mouse IgG1-HRP (Jackson ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... primary mouse myoblasts and human immortalized myoblasts were transfected with 60nM final of siRNAs (Sigma Aldrich) using LipoRNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC lines were maintained on irradiated MEFs in hESCs medium [DMEM/F12 (Sigma-Aldrich, D6421) supplemented with 20% KO Serum Replacement (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by i.p injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human alveolar type II epithelial cell line A549 was purchased from Sigma-Aldrich (Poole, UK) and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... which is made by supplementing RPMI Medium 1640 with 5 µg/mL human insulin (Sigma-Aldrich), 5 µg/mL human transferrin (Sigma-Aldrich) ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Immunology 2019Quote: ... they were intraperitoneally injected with 2.5 iu human chorionic gonadotropin (HCG, Sigma Aldrich, St. Louis, MO) and immediately mated with male mice overnight (16 hours) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on glass coverslips coated with 50µg/mL human type IV collagen (Sigma C7521) 24 to 48h before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... META-enriched cultures were isolated via resistance to complement-mediated lysis in 20% human serum (Sigma) for 30min at 37°C and validated via sherp expression [21,23].
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bioRxiv - Immunology 2019Quote: Human and mouse omentum samples were fixed for one hour on ice in 10% NBF (Sigma) and then permeabilized in PBS 1% Triton-X 100 (Sigma ...
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bioRxiv - Genomics 2021Quote: DNA from human keratinocyte cells was extracted using the “GenElute-Mammalian Genomic DNA Miniprep Kit” (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then 50 μL of alkaline phosphatase-conjugated secondary goat anti-Human anti-IgG (Sigma Cat # A9544), anti-IgA (Abcam Cat # AB97212) ...
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bioRxiv - Bioengineering 2021Quote: Immortalized human neural progenitor cells derived from the ventral midbrain (ReNCell VM) were obtained from Millipore. Cells were expanded on laminin-coated tissue culture flasks ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (A1653) and mouse serum albumin (A3139) were purchased from Sigma (St Louis, MO). Fluorescently labeled human serum albumin (HS1-S5-1 ...
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bioRxiv - Neuroscience 2020Quote: ... were co-transfected into U87 human primary glioblastma cells by using ESCORT V transfection reagent (Sigma). The fold increase in relative luciferase activity is a product of the luciferase activity induced by TCF7l2-S2 or TCF7l2-E2 (backbone vector pCMV ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
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bioRxiv - Cell Biology 2021Quote: ... HAP1 cells were transfected with 600 ng/ml human VRK2 MISSION® esiRNA (EHU070731, Sigma-Aldrich) using Lipofectamine RNAiMAX (13778075 ...
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bioRxiv - Cell Biology 2021Quote: ... Fibronectin-coated coverslips were prepared by first covering them with 100 µL of human (Sigma F0556) or bovine (Sigma F01141 ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
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bioRxiv - Systems Biology 2021Quote: Dispersed human islets and iPSC-beta cells were exposed to 0.5 mmol/L palmitate (Sigma-Aldrich) for 24 h and 48h ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
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bioRxiv - Microbiology 2021Quote: ... ChIP MIC in serum was determined in the presence of 50% (heat inactivated) human serum (Sigma) and 50% MHB-II ...
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bioRxiv - Microbiology 2019Quote: ... The immortalized human cerebral microvascular endothelial cell line hCMEC/D3 (59) was purchased from Millipore Sigma and maintained in Endothelial Cell Growth Basal Medium-2 (EBM2 ...
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bioRxiv - Cell Biology 2020Quote: ... DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen) to a retroviral vector compatible plasmid (PPRIG ...
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bioRxiv - Cell Biology 2021Quote: ... FN coating was accomplished by adding phosphate buffered saline (PBS, Cellgro) and human plasma fibronectin (Millipore) to six-well plates (Corning) ...
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bioRxiv - Biochemistry 2022Quote: ... Type O+) at 4% haematocrit in NaHCO3 and AlbumaxII/human serum supplemented RPMI (RPMI (Sigma Aldrich), 25 mM HEPES (GIBCO) ...
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bioRxiv - Bioengineering 2022Quote: ... Scaffolds were washed with PBS and incubated with or without 100 nM human insulin (Sigma-Aldrich) in 100 μL glucose-free DMEM/F12 for 1 hour at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in 12-well plates coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich ...
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bioRxiv - Pathology 2022Quote: ... and fibrin deposits using DyLight488-anti-human/mouse fibrin antibody (8 μg, clone 59D8, Sigma-Aldrich). All antibodies and dyes were injected intravenously 15 min prior to intravital imaging ...
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bioRxiv - Genomics 2022Quote: ... full length human LINC00881 was cloned into p3XFLAG-CMV-7 (Millipore Sigma, St. Louis, MO, USA) vector and amplified in DH5α strain of Escherichia coli (E coli ...
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bioRxiv - Bioengineering 2022Quote: ... The antibodies used were a) purified CHO h-IgG b) IgG from human serum (Sigma-Aldrich, resuspended in deionised H2O and stored at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.324 and 0.108ng of a reference DNA (Human genomic DNA from buffy coat, Sigma cat# 11691112001) are included in each PCR run so that the quantity of targeted templates in each research sample can be determined relative to the reference DNA sample by the standard curve method ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse Tollip (TRCN0000066136 and TRCN0000066137) and human RIOK1 (TRCN0000295923 and TRCN0000037399) were obtained from Millipore Sigma.
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bioRxiv - Immunology 2020Quote: ... permeabilized RBCs were stained with anti-human spectrin antibody (1 in 50 dilution; S3396, Sigma Aldrich) concurrently with GNA lectin (8 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... followed 47-48 hours later with 5 IU of human chorionic gonadotrophin (Sigma-Aldrich, CG10-1VL) before being bred ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (SIGMA, male AB, H-4522), 1% l-glutamine ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (Sigma, male AB, H-4522), 1% l-glutamine ...