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Citations for Millipore Sigma :
3201 - 3250 of 10000+ citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... RIPK3 inhibitor GSK 872 (N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine) was purchased from Millipore Sigma and frozen stocks were maintained in DMSO ...
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Developmental Biology 2021Quote: ... inactivated with 10 µg/ml mitomycin C and seeded at 4 × 105 per 6 cm dish) in DMEM/F12/KSR medium (D-MEM/F12 (Sigma) supplemented with 20% KSR additive ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT cells were plated in 10-cm dish with culture medium containing only blasticidin S following co-transfection with Flp recombinase expressed plasmid pPGKFLPobpA and tetracycline-responsive plasmid pcDNA5FRT/TO containing TDP-43 Q331K or pcDNA5FRT/TO only as isogenic sham control in the ratio 6:4 using PEI (polyethyleneimine; Sigma) transfection method ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... i.p.) young adult (4-week-old) C57BL/6 male mice and transferred to ice-cold DMEM/F12 media (Sigma Aldrich) in 6 well plates ...
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bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Neuroscience 2021Quote: ... 1% indole (Sigma W259378), 1% (-)-dihydrocarveol (Sigma 37278) ...
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bioRxiv - Microbiology 2021Quote: ... and Indole (Sigma-Aldrich) were added to cells as needed 2 h before infection.
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... before fixation with 4% paraformaldehyde/ 4% sucrose (v/v), and subsequently permeabilized (DPBS, 0.2% triton X-100 (Sigma-Aldrich #9002-93-1)) ...
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bioRxiv - Cell Biology 2020Quote: ... and was centrifuged twice at 300 g for 10 min at 4 °C in a Sigma 4-16KS centrifuge (Sigma Laboratory Centrifuges) to remove cells and the supernatant was stored at −80 °C until use.
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 500nM 4-hydroxytamoxifen (4-OHT) for 72h in 5i+LIF (Supplementary Table1) with 100 μM apoptosis inhibitor Z-vad (Sigma-Aldrich®) and 50 μM of necroptosis inhibitor Necrostatin-1 (Sigma-Aldrich®) ...
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bioRxiv - Neuroscience 2022Quote: ... and the cochleae were fixed in 4% paraformaldehyde (PFA) overnight at 4 °C and then decalcified in 10% EDTA (#798681, Sigma, Darmstadt, Germany) in PBS for 2–3 days at 4 °C ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Cancer Biology 2019Quote: ... Established cell lines were propagated in DMEM containing 10% FBS and maintained with 100nM of (Z)-4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich H7904) and routinely evaluated for mycoplasma contamination ...
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bioRxiv - Genomics 2021Quote: ... three times for 30 minutes each at room temperature and transferred into freshly made X-gal staining solution (4 mM potassium ferricyanide [Sigma-Aldrich, catalog no. P3667], 4 mM potassium ferrocyanide [Sigma-Aldrich, catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... and perfused with 40 ml of 0.1 M/L phosphate-buffered saline and 40 ml of 4% (w/v) PFA (Sigma, 30525-89-4) 24h after social interaction ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to an OD600 of 0.1 the next day and grown up to a mid-log phase (OD600 of 0.8) and labeled with 4-thiouracil (4-tU, Sigma, 2M in DMSO) for 6 min at a final concentration of 5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... cerevisiae was spiked with S. pombe total RNA (4:1 ratio S. cereviasiae : S. pombe)treated with 4-tU 500 μM final concentration (Sigma-Aldrich, 440736). The biotinylation was performed with 200ul EZ-link-HPDP-biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3x with 1x PBS and incubated with appropriate primary antibody (Table 4) diluted in 4% BSA (Sigma, A9418-50G) in 1x PBS overnight at 4C ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed with 500 μl of PBS and fixed with 500 μl of fixative solution (4% formaldehyde, Sigma, and 4% acrylamide, Sigma, in PBS) overnight (O/N ...
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bioRxiv - Cell Biology 2023Quote: ... The organoids thus generated were re-plated at 1:4 split ratio and exposed to 400 nM 4-hydroxy tamoxifen (Sigma; Catalog #H7904) in medium to achieve the deletion of Cbl and Cblb ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL of sample was mixed with 4 µL of TLC-MALDI buffer (MB, 5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich), 25% acetonitrile ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Developmental Biology 2024Quote: ... Tg(actab2:loxP-BFP-STOP-loxP-dsRed)sd27 fish were treated overnight at 5 dpf with 5 μM (Z)−4-Hydroxytamoxifen (4-OHT) (Sigma-Aldrich H7904) in EM ...
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bioRxiv - Cancer Biology 2022Quote: ... SNB19 and BTSC 233 cells were treated with 10 mM 4-methylthio-2-oxobutyric acid (MTOB, Sigma) for 24h ...
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bioRxiv - Neuroscience 2021Quote: ... ablation efficiency was confirmed using the fluorescent dye 2-4-(dimethylamino)styryl-N-ethylpyridinium-iodide (DASPEI)(Sigma). Larvae were immersed in 0.005% DASPEI in E3 for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubïgen larva from each treatment group were incubated in 4ug/ml Diasp (2-Di-4-Asp, Sigma) and 0.3 ug/ml DioC6 (3,3-dihexyloxacarbocyanine iodide ...
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bioRxiv - Developmental Biology 2020Quote: ... larval sections were counterstained with DAPI (4′,6diamidino-2-phenylindole, Sigma-Aldrich; 1:1000 μL PBS dilution) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then treated with 2 mM (or 4 mM for HCoV 229E) bromouridine (BrU; Sigma Aldrich) and 15 μM actinomycin D (ActD ...
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bioRxiv - Plant Biology 2022Quote: ... they were supplemented once per week with 1/4 strength Hoagland No.2 media (Sigma Aldrich H2395). For tissue harvesting ...
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bioRxiv - Genetics 2020Quote: ... Intestinal organoids were induced on day 2 after splitting using 800 nM 4-OH tamoxifen (Sigma H7904) for 24 h.
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bioRxiv - Biochemistry 2021Quote: ... 250 μL Protease Inhibitor Cocktail (18 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) (Sigma, No. A8456), 1.7 mM bestatin (Sigma ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... and stained overnight at 4°C with 2 µg/ml Hoechst 33342 (Sigma Aldrich, St. Louis, MO) and a 1:1000 dilution of anti-phospho-histone H3 (pHH3 ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 4-amino-5 methylamino-2’ s,7’-difluorofluorescein (DAF-FM) diacetate (Sigma, D1946). DAF fluorescence intensities were measured every 5 minutes for a total of 60 minutes using a SpectraMax iD3 (Molecular Devices) ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed twice with DPBS and then fixed with 2 mL/well 4% paraformaldehyde (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The parasites (ring stage; 2-4 hpi) were tightly synchronized with 5% (v/v) D-sorbitol (Sigma) and then monitored by Giemsa staining of methanol-fixed blood smears ...
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bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St ...