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Citations for Millipore Sigma :
3151 - 3200 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 20 U/mL IL-2 (AIDS Reagent Program, Germantown, MD) and 1% penicillin/streptomycin (Sigma- Aldrich, St. Louis, MO), Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by 8% to 12% SDS–PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Blocking and probing with antibodies were performed as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins (25-50µg) were resolved on 8 to 15% SDS-PAGE and blotted onto PVDF membrane (Millipore). Membranes were blocked for 1 h at room temperature with TBS-0.1% Tween20 and 5% milk or 1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated with purified lentiviral vectors in the presence of 8 μg/mL polybrene (Sigma). The details of the developed cells are described in the key resource table.
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bioRxiv - Evolutionary Biology 2022Quote: ... Coverslips were incubated with digestion buffer supplemented with 8 U/ml of proteinase K (Sigma-Aldrich, P2308) at 37 °C for 2 – 3 h.
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bioRxiv - Cell Biology 2022Quote: ... Target cells were then infected through incubation in media containing 8 µg ml−1 polybrene (408727, Sigma) for 16 h to 20 h ...
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bioRxiv - Biochemistry 2022Quote: ... at a volume of 3.0 mL with a 9 mm x 8 mm spin bar (Sigma Z363545) cuvette stirrer ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration series reached from 0.5 μM to 8 μM for the positive control Spermine (Sigma Aldrich) and compound 12/Spermidine ...
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bioRxiv - Cell Biology 2019Quote: ... From 8 hpf the medium was supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) to prevent pigmentation ...
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bioRxiv - Biochemistry 2019Quote: Clindamycin [CAS: 21462-39-5] and 5-fluorouracil [CAS: 51-21-8] were purchased from Sigma-Aldrich, St ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Animals were immobilized using 1 mL of tetramisole hydrochloride (200 mM) (Sigma-Aldrich cas. 5086-74-8) in S Basal ...
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.15 M NaCl and 2 mM EDTA pH 8 and subsequently eluted with 95% formamide (Sigma, F9037) in water ...
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bioRxiv - Neuroscience 2019Quote: ... medium was changed to mTeSR supplemented with 2,5 μl lentivirus and polybrene 8 μg/μL (Sigma-Aldrich). On day 2 ...
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bioRxiv - Bioengineering 2019Quote: ... sputum aliquots were diluted 10-fold the original volume in 8 M guanidine-HCl (Sigma Aldrich, MO). Samples were pretreated with 10% (v/v ...
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bioRxiv - Genomics 2021Quote: ... 100 µL of filtered must were incubated with 3 µL of DEEM (Sigma-Aldrich 87-13-8) for 30 min in a sonication bath at room temperature in a solution containing 580 µL of borate buffer (pH 9 ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed 8 times and avidin-peroxidase (2.5 μg/mL in PBS / 10 % FCS. Sigma) was added for 30 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PDB/gut mixture was then mixed with gelatin (Sigma-Aldrich, Germany, Cas number: 9000-70-8) to acquire solid feeding blocks ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting analysis was carried out as previously described.(8) Antibodies against Actin (Sigma, A2066; dilution 1:4,000), DOT1L (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... Lentiviral transduction was performed overnight in TeRS-E8 medium with 8 µg/mL of polybrene (Sigma-Aldrich). On day 1 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged at 1/8 ratio every 3 or 4 days with Accumax (Millipore, ref. SCR006).
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bioRxiv - Cancer Biology 2020Quote: ... the eIF4EWT and eIF4EKI mice (6-8 weeks old) were treated with 2 μl 4-HT (Sigma) at 5 mM working concentration for 3 continuous days ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µL of Fluo-4AM working solution were mixed with 8 µL of Pluronic F-127 (Sigma). The mixture was vortexed and diluted in 2 mL growth medium ...
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bioRxiv - Cancer Biology 2019Quote: ... The medium was removed and replaced with 110 μl fresh medium containing 8 μg/ml Polybrene (Sigma) added to each well to increase the transduction efficiency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: DINCH (CAS No. 166412-78-8; molecular formula: C6H10(CO2C9H19)2) was purchased from Sigma (purity ≥95 %). The physical chemical properties of DINCH are as follows ...
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bioRxiv - Microbiology 2021Quote: ... 4.6 ml of 48 h viral supernatant mixed with 14.1 μl of 8 μg/ml polybrene (Sigma) was added to 1.4-2.8 x106 ECs cultured to 20-30 % confluence in a T75 flask ...
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bioRxiv - Cancer Biology 2020Quote: ... filtered with 0.45 µm filters and supplemented with 8 µg/mL polybrene (EMD Millipore, Burlington, MA, USA). Serial transductions (48 and 72-hour supernatants ...
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bioRxiv - Cell Biology 2021Quote: ... Thiazole orange stained PRP was diluted 1 in 8 using (i) saline with apyrase (0.04U/mL, Sigma), prostaglandin E1 (PGE1 ...
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bioRxiv - Biophysics 2020Quote: ... SNAP-stained cells were mounted on Nunc™ Lab-Tek™ II Chambered Coverglass 8 wells (Sigma) coated with Lectin ...
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bioRxiv - Microbiology 2020Quote: ... 1 µl of matrix (α-Cyano-4-hydroxycinnamicacid, HCCA, CAS Number 28166-41-8, Sigma-Aldrich, Switzerland) was added ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Cell Biology 2021Quote: The cells were grown in an 8-well chamber slide (Millicell EX slide, Merck Millipore, Burlington, MA) to early confluency ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in Hank’s Balanced Salt Solution and seeded in 8-well poly-Lysine-coated (0.025%; Sigma-Aldrich) chamber slides (Falcon ...
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bioRxiv - Molecular Biology 2022Quote: ... A549-ACE2 cells were seeded at 20,000 cells per well of an 8-well chamber slide (Millipore), which was pre-coated with Matrigel (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... and 8 M Urea was added to a 10 kDa spin filter column (Microcon, Merck-Millipore, USA), on-filter reduced ...
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bioRxiv - Microbiology 2022Quote: ... were transduced into HeLa cells at MOI of 1.0 with 8 μg/ml of polybrene (Sigma-Aldrich) by centrifuging cells and vectors at 2,300 x rpm for 1h at 22°C ...
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bioRxiv - Microbiology 2022Quote: ... DNA pellets were resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8, Sigma) and stored at -20 °C until used for sequencing.
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bioRxiv - Cell Biology 2022Quote: ... The following compounds were used at the indicated concentrations: TR100 (5, 8 or 10 μM, Sigma Aldrich), CK666 (25 μM ...
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bioRxiv - Immunology 2022Quote: ... Cells were then transduced with lentiviruses in the presence of 8 μg ml-1 polybrene (Sigma, H9268) for 24 h before the replacement of the fresh culture medium ...
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bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown to 70% confluence on 8-well glass culture slides (Merck Millipore, Tullagreen, Carrigtwohill, Ireland), washed ...
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bioRxiv - Immunology 2022Quote: ... followed by two rounds of transduction of the recipient cell line with 8 μg/ml Polybrene (Millipore). Cells stably expressing desired construct were obtained by selection in complete medium supplemented with 2 μg/ml puromycin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... adult (6-8-wk-old) Lrig1-CreERT2/+;Apc-flox/+ mice were intraperitoneal-injected with 2mg tamoxifen (Sigma) in corn oil for 3 consecutive days ...
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bioRxiv - Physiology 2020Quote: ... Cells were treated with Dex (Sigma-Aldrich, D4902, 1×10−7 M or 1×10−8 M) and 991 (Spirochem ...
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bioRxiv - Genomics 2019Quote: Approximately 8-9 × 106 ESCs (per sample) were treated with cycloheximide (0.1 mg/ml; Sigma, 01810-1G) for 1 minute prior to trypsinization and cell lysis ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Bioengineering 2020Quote: ... the glass fiber capture membrane was submerged for 6-8 hours in trifluoroacetic acid (TFA, Sigma-Aldrich), and dried at room temperature overnight before assembly ...
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bioRxiv - Physiology 2020Quote: ... in which maximum projected images of F-actin channel (mesenteries) were filtered by Gaussian Blur (Sigma = 8), thresholded with the same value to automatically select oocyte tissue and manually curated to measure the area.
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bioRxiv - Cancer Biology 2019Quote: ... promoter with an upstream ubiquitous chromatin opening element) in the presence of 8 μg/mL polybrene (Sigma). A pure polyclonal population of dCas9-KRAB expressing cells was generated by 2 rounds of fluorescence activated cell sorting (FACS ...