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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Cell lines were grown at 37°C in either RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich, UK), 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... At 1 h post infection (hpi) the infection medium was exchanged with DMEM/10% FCS containing 100 µg/ml gentamicin (GEN, Sigma-Aldrich G1397) and increasing concentrations of CIP (0 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed brain samples were placed securely one at a time in a custom-made MR-compatible holder and immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, UK). Samples were scanned in a random order ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS; PAA, Cölbe, Germany) and 50 U/ml penicillin plus 5 μg/ml streptomycin (Sigma-Aldrich, Munich, Germany). Peripheral blood mononuclear cell (PBMCs ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL 5% FC-40 oil (RAN Biotechnologies, CN 008-FluoroSurfactant-5wtF) was added to the bottom and 50 µL mineral oil (Sigma, CN M5904) was added on the top ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 % fetal calf serum (FCS) as well as Pen/Strep (50 U/ml penicillin + 50 μg/ml streptomycin; Sigma-Aldrich, USA). After centrifugation at 900 g ...
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bioRxiv - Microbiology 2024Quote: ... the infected cells were washed twice and further incubated in DMEM/10% FCS containing 100 μg/ml gentamicin (GEN, Sigma-Aldrich G1397) to remove extracellular bacteria ...
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bioRxiv - Immunology 2022Quote: ... and transferred in the digestion medium (HBSS with Ca2+ Mg2+ - Fetal Calf serum (FCS) 2% - DNase I 0.1mg/mL (Sigma-Aldrich, ref 11284932001) – Collagenase IV 0.5mg/mL (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Immunology 2023Quote: ... CD44-FUT7-Fc collected from cell culture medium was concentrated 200-fold using Amicon® Ultra-15 centrifugal filter units (Millipore Sigma), and 10µl of this concentrate was added to block the HUVEC substrate for 1h before performing the rolling assay at 1dyn/cm2 ...
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bioRxiv - Immunology 2023Quote: ... 110-HG) in the presence or absence of 1 μg mCD152/Fc (CTLA4-Ig, mouse IgG2a, non-cytolytic) chimera (Sigma-Aldrich, C4358). The anti-human IgG Fc Antibody (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MB49 was cultured in RPMI media (Biochrom GmbH) supplemented with 10% FCS (Biochrom GmbH) and 50 μg/ml Gentamycin (Sigma, Darmstadt, Germany).
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bioRxiv - Cell Biology 2024Quote: ... were thawed and washed with Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with 5% foetal calf serum (FCS) once before incubating with 1mg/ml collagenase P (Sigma Aldrich, UK) and 100μg/ml DNase I (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... protein were combined with a mixture of 50 % (v/v) protein A and 50 % (v/v) protein G beads (Sigma-Aldrich Inc., Cat#P9424 and Cat#P3296) and 0.5-1 μg antibody in lysis buffer overnight at 4 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we also determined whole-body triacylglyceride (TAG) content (in µg per fly) using a serum triglyceride determination kit (Sigma Aldrich; Tennessen et al. 2014). For each cage and treatment ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... we added both the XPoSE-tag and neuronal nuclear marker NeuN (anti-NeuN Mouse mAb clone A60, MAB377X, Millipore Sigma, RRID: AB_2149209, 1:500) We incubated the samples with antibody for 15 minutes at 4 °C on a rotating mixer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore, Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Genomics 2020Quote: ... of GAPDH (cytoplasmic protein) and Histone H3 (nuclear protein) using rabbit anti-GAPDH antibody (1:5000, Sigma) and rabbit anti-histone H3 antibody (1:10000 ...
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bioRxiv - Microbiology 2019Quote: Quantified protein (between 80-160 μg) was incubated with protein-specific antibodies (either R anti-HA (Sigma) or anti-NS4A ...
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bioRxiv - Biochemistry 2021Quote: Protein concentrations were measured with the Bio-Rad Protein Assay Kit using bovine serum albumin (Sigma-Aldrich) as reference.
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bioRxiv - Cell Biology 2021Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Cell Biology 2022Quote: ... BCA was performed on the freshly isolated proteins using a Pierce BCA protein assay kit (Sigma, 23227) to determine the protein concentration ...
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bioRxiv - Cell Biology 2022Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Systems Biology 2022Quote: ... The dried pellet was used for protein analysis using Bicinchoninic Acid Kit for Protein Determination (Sigma Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... The purified protein was separated by SDS-PAGE and protein was excised after InstantBlue™ (Sigma-Aldrich) staining ...
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bioRxiv - Immunology 2019Quote: ... Protein concentration was determined using BCA protein assay reagent (Pierce Chemical Co.) with bovine serum albumin (Sigma) as the standard.
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bioRxiv - Cancer Biology 2020Quote: ... cell lysate containing about 500 μg protein was cleared with 30 μl Protein A (Millipore, Billerica, MA) for 4 h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... 50mM HEPES pH6.6-8.5 depending on protein isoelectric point) containing complete EDTA-free protein inhibitors (Sigma-Aldrich), centrifuged for 10 min at 1,620 rcf at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Protein estimation was done on the depleted plasma protein samples using the Bradford assay (Sigma-Aldrich, USA). In the SWATH-MS analysis following steps like reduction ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: Mice were immunized intradermally (i.d.) in the ear with 10μg OVA protein or KLH protein (Sigma Aldritch) emulsified in complete Freund’s adjuvant (CFA ...
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bioRxiv - Cell Biology 2022Quote: ... or Protein G Sepharose beads (Protein G Sepharose® 4 Fast Flow, Sigma-Aldrich, GE17-0618-01) used for anti-Ollas antibody IPs ...
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bioRxiv - Immunology 2022Quote: ... with protein concentration determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Sigma, UK).
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: FLC-Venus protein stability was assayed with the de novo protein synthesis inhibitor cycloheximide (C1988, Sigma-Aldrich) following the procedures in (Zhao ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were extracted from the conditioned medium using the protein precipitation kit (#2100, Millipore Sigma, Burlington, MA) following the manufacturer’s specifications ...