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Citations for Millipore Sigma :
3101 - 3150 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then filtered through a 10kDa protein filter (MRCPRT010, Sigma Aldrich) via centrifugation at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The dialyzed protein was concentrated using a 30 kDa MWCO concentrator (Millipore) and was further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Systems Biology 2023Quote: ... We then transferred proteins to a low background PVDF membrane (Millipore, IPFL00010) for 15h at 15V using the TRIS-Glycine buffer (48 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... proteins were electrotransferred to a polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA) using a semi-dry transfer blotter (Atto) ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were eluted in Flag peptide (Sigma, F4799, 150 ng/μl) for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Pure proteins were concentrated using 10 kDa Amicon Ultra membrane concentrators (Millipore), flash-frozen in liquid nitrogen and stored at −80℃.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using the Bicinchoninic acid assay (BCA, Sigma-Aldrich). Proteins were then denaturated with Laemmli Buffer (Tris pH 6.8 (200mM) ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Both the proteins were concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... total protein was visualized using PonceauS staining (Sigma-Aldrich, St. Louis, MO), blocked using 5% non-fat milk powder in tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg protein per sample were reduced with 10 mM DTT (Sigma) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, USA), which was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg protein per sample was reduced with 3 mM TCEP (Sigma) and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P, Millipore, USA) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Polyclonal antibodies from antiserum were purified using Protein A Sepharose (Sigma, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by immunoblotting using anti-Glutathione-S-Transferase (Sigma-Aldrich), anti-polyHistidine-Peroxidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using Amicon Ultra-4 10 kDa MWCO (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...
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bioRxiv - Molecular Biology 2023Quote: ... protein synthesis was inhibited by blocking translation step using cycloheximide (Sigma, #C7698) and MMP activity was inhibited by ilomastat (GM6001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was extracted in RIPA buffer containing protease inhibitor mixture (Sigma) and phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were to the following proteins: Rat anti-MBP (Millipore, MAB386), Rabbit anti-SMI32 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Following a reduction of proteins with 10 mM DTT (Sigma-Aldrich, Germany) in 100 mM TEAB at 57 °C for 30 min and dehydration of gel pieces ...
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bioRxiv - Immunology 2023Quote: ... The protein in PBS was 0.22um filtered using spin filters (Millipore Sigma) prior to Size exclusion Chromatography (SEC) ...
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bioRxiv - Microbiology 2023Quote: ... The elution containing native DnaN protein was concentrated by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Cells for protein extraction were lysed using RIPA lyses buffer (Sigma #R0278) containing protease and phosphatase inhibitor cocktails (cOmplete™ ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies to the following proteins were used in this study: 53BP1 (Millipore), γ-H2AX (Ser 139 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with ReadyBlue Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed on a ChemiDoc Imaging System by Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P, Millipore) in a wet tank apparatus ...