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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: The protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich C7698) (Pedreira et al. ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: GST fusion proteins were expressed in Rosetta cells (Novagen) at 18°C for 16 hours after induction with 500 μM IPTG at an OD600 of 0.6 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were reduced with dithiothreitol (DTT, 5 mM; Sigma) at 55°C for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... Protein gels were wet transferred to PVDF membrane (Millipore). Specific proteins were detected using 1:1000 dilution of Myc-Tag (9B11 ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-microtubule associated protein 2 (MAP2, 1:200, Millipore), mouse monoclonal anti-ankyrinG (AnkG ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined using a BCA assay (Sigma) and biotinylated proteins were pulled down using a corresponding concentration of Dynabeads Streptavidin C1 (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein loading control tubulin (Merck, Sigma-Aldrich). Immuno-reactive complexes were detected using HRP-conjugated secondary antibodies (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were overexpressed from Escherichia coli BL21 (DE3) (Novagen) cells transformed with the corresponding expression plasmids (Table S6) ...
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bioRxiv - Microbiology 2020Quote: Parasite protein extracts were pretreated with Benzonase nuclease (Sigma) (70 U to protein extract from 1 L culture at a final concentration of 90 U/mL ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM NaVO3) with protein inhibitor cocktail (Sigma®) followed by total protein quantification ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 15% trichloroacetic acid (Sigma-Aldrich) for 30 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit anti-Protein A antibodies (Sigma Cat. No P3775) was used for ChIP at a final concentration of 20 μg/ml of immunoprecipitate (IP) ...
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bioRxiv - Immunology 2020Quote: ... the proteins were transferred to PVDF membranes (Millipore, ISEQ00010) and further incubated with the indicated primary and secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-green fluorescent protein antibody (Sigma, SAB4301138). In most cases ...
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bioRxiv - Genetics 2020Quote: ... Proteins were reduced in 10 mM DTT (Sigma-Aldrich) for 1h at 56°C and then alkylated with 5mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was transferred onto Immobilon-FL PVDF Membrane (Millipore) at 100 V for 1.5 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... using biotynilated HA-binding protein (385911, Sigma Aldrich, USA) and biotynilated GSLII (B-1215 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were transferred to Amersham Protran nitrocellulose membranes (Sigma) for Western blot analysis ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010) in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... protein was transferred to a PVDF membrane (Millipore, IPFL00010). The blot was blocked for 1 hour in LI-COR Odyssey Blocking Buffer PBS (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... The protein bands were transferred to PVDF membranes (Millipore). After being blocked with 5% nonfat dry milk ...
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bioRxiv - Bioengineering 2022Quote: ... A four-molar excess of scaffold protein (MSP1E3D1, Sigma) was added to the protein-lipid mix ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were probed with antibodies against FLAG M2 (Sigma), Cdc2 (Santa Cruz Biotechnologies SC-53217) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Millipore, IPFL00010) via the wet-transfer procedure ...
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bioRxiv - Genomics 2022Quote: ... bead-bound proteins were eluted in Laemmli buffer (Sigma) and resolved on a 4–20% SDS-polyacrylamide gel (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 30 μl of protein A agarose beads (Sigma). Samples were subjected to SDS-PAGE and immunoblot analysis as described (16,20,23,82,86–87) ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted proteins were precipitated with trichloroacetic acid (Sigma) and subject to MS analyses as described previously 13.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore, IPVH85R) at 120V for 65 min at 4C in 1x tris-glycine-methanol transfer buffer [25 mM tris base ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were eluted in 2X Laemmli (Sigma-Aldrich) or deglycosylated by an 1h treatment with PNGase F (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) final concentration at 95°C for 10 minutes and alkylated with iodoacetamide (Pierce,40 mM final 30 minutes) ...
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bioRxiv - Neuroscience 2022Quote: ... 30ul of equilibrated Protein G – agarose beads (Sigma 15920010) were added to the lysates and incubated further for 3 hours.
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bioRxiv - Neuroscience 2022Quote: ... and proteins were extracted in RIPA buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets ...
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein synthesis inhibitor cycloheximide was purchased from Sigma-Aldrich. USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was digested with sequencing grade trypsin (Sigma; #T6567) and sequencing grade LysC (Wako ...
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bioRxiv - Systems Biology 2022Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 Vfor 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to methanol activated PVDF (Millipore) membranes (350 mA for 60 minutes) ...
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bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...