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Citations for Millipore Sigma :
3101 - 3150 of 4323 citations for Recombinant Human HAPLN1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Human Embryonic 293T (HEK293T) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2019Quote: HeLa human epithelial cells were recovered from freezer stocks in 10% dimethyl sulfoxide (DMSO, Sigma) and full growth media consisting of Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... blocked with 100 μl/well of 1% human serum albumin (HAS) in PBST (Sigma-Aldrich) for 1 h at RT and then washed 4 times with 100 μl/well of PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Semaphorin 6B cytoplasmic tail (aa 616-1021) (SEMA6B, amplified from human brain cDNA library, Novagen); Semaphorin 6D cytoplasmic tail (aa 684-1073 ...
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bioRxiv - Developmental Biology 2019Quote: ... mice received a second IP injection with 5 units of human chorionic gonadotropin (HCG) (Sigma). Immediately after injection females were placed into the cages of stud males ...
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bioRxiv - Cancer Biology 2020Quote: ... SCC-9 and SCC-4 (human tongue OSCC) cell lines were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using goat-anti-human-IgM-HRP (1:1000) (Sigma catalogue no. A6907) and wells were washed with PBS-T and one-step 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2021Quote: ... (3) TexMACS™ medium (Miltenyi) was supplemented with 3% heat-inactivated human AB serum (Sigma) and 1X Penicillin Streptomycin solution (TAC) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Pre-clinical Repository) ...
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bioRxiv - Bioengineering 2021Quote: ... Fluorescein isothiocynate human serum albumin (FITC-HSA) was purchased from Sigma Aldrich (Oakville, ON, Canada). Milrinone was purchased from Selleck Chemicals (Burlington ...
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bioRxiv - Neuroscience 2020Quote: ... the frogs previously injected with PMSG were injected with human chorionic gonadotropin (hCG, 400UI, Sigma) to induce egg laying ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with pLKO.1 shRNA plasmid (Sigma, Mission human genome shRNA library) and packaging plasmids-delta 8.9 ...
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bioRxiv - Cancer Biology 2020Quote: ... human cells iso-lated and treated in vitro with 50nM TAM (Sigma-Aldrich, H7904-25G) to induce recombination and shRNA expression ...
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bioRxiv - Cell Biology 2021Quote: ... 32 human Nups were systematically depleted using two different commercially available siRNA pools (Dharmacon; Sigma). siRNAs were transfected into the HeLa cells at a 50 nM final concentration using RNAiMAX (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in AIM V (Thermo) supplemented with 5% human serum (Sigma #H3667). PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Systems Biology 2020Quote: ... 35mm glass Mat-tek dishes were coated with 10µg/ml Fibronectin (human plasma, Sigma #F0895) for 1 hour ...
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bioRxiv - Physiology 2021Quote: Plasma FGF21 (pg/ml) was measured using a human FGF21 ELISA kit (Millipore, Billerica, MA). In Experiment 2 ...
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bioRxiv - Microbiology 2021Quote: ... a human IgG1 kappa standard purified from myeloma plasma (in duplicates per plate, Sigma-Aldrich) and serum samples (starting at a 1:20 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
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bioRxiv - Cancer Biology 2020Quote: HCT116 human colon adenocarcinoma cell lines were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma). Cell culture media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2021Quote: ... 2×106 cells/ml sperm were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Human PBMC were isolated from the peripheral blood of healthy volunteers using Ficoll (Sigma-Aldrich) density centrifugation in Greiner LeucoSep-tubes (Sigma ...
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bioRxiv - Genetics 2022Quote: ... the corresponding cDNA fragment of human FLCN was cloned in the pET-30 vector (Novagen) and the His-tagged fusion protein was expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... The COCs were incubated in human tubal fluid (HTF) medium (Millipore Corp; MR-070-D) with appropriate concentration of sperm obtained from 1 hour capacitation ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX, C3657, Sigma-Aldrich, St Louis, MO) was pre-solubilized in aqueous acid and diluted in carbonate coating buffer ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Bioengineering 2019Quote: Genotoxicity assay was determined using Hek 293T cells (Human embryonal kidney cells, Sigma-Aldrich, USA) according to Comet assay as previously described [31] ...
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bioRxiv - Cell Biology 2020Quote: ... pre-coated with human type IV collagen (Cat# C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day −2) ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Microbiology 2020Quote: Human foreskin fibroblasts (Hff1; ATCC® SCRC-1041™) were maintained in DMEM (Sigma-Aldrich) supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin and 100 µg/mL streptomycin.
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lines and primary human fibroblasts (MRC5) were cultured in DMEM (D6546, Sigma-Aldrich) with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by intra-peritoneal injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human liver hepatocellular carcinoma (HepG2) cells were cultured in MEM (Sigma, St. Louis, MO, USA) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (human embryonic kidney) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma D6429) containing 10% FBE ...
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bioRxiv - Neuroscience 2021Quote: ... full length human NPY peptide (10 pmol in 1x PBS, Sigma-Aldrich, Cat. No. N5017), anti-CART antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and subsequently incubated for 10 min in the presence of 5 % human serum (Sigma-Aldrich). Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs ...
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bioRxiv - Biochemistry 2021Quote: The human Hsf1 and Hsf1-GFP expression constructs were cloned into a pET vector (Novagen). Hsf1 CS mutant (C35S/C103S/C153S/C373S/C378S ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Cell Biology 2020Quote: Mouse embryonic lungs and human bronchial tissue were fixed overnight in 4% PFA (Sigma; 441244) at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... A standard curve was prepared using human IgG1 kappa purified from myeloma plasma (Sigma-Aldrich). Serial dilutions of the IgG standard (in duplicates ...
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bioRxiv - Immunology 2021Quote: The human lung adenocarcinoma A549 cells (ATCC, Manassas, VA) were grown in DMEM (Sigma-Aldrich) medium containing 10% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2020Quote: Fresh or cryopreserved human PBMC’s were isolated from heparinised whole blood by Ficoll gradient (Sigma). Cells were incubated for 1hr (37°C ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... Aβ42 levels were measured using a High Sensitivity Human Amyloid β42 ELISA Kit (Millipore, #EZHS42).
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bioRxiv - Developmental Biology 2021Quote: Sectioned tissue derived from regenerated human skin was fixed with 10% formalin solution (Sigma HT5012) for 12 minutes ...