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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285) and intact cell respiration was normalized to milligrams of total intact cell protein.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285).
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bioRxiv - Biophysics 2021Quote: Spin-labeled BmrCD mutants were concentrated to 70-100 µM using Amicon Ultra-100 kDa centrifugal filters (Millipore) and incubated with nucleotides or Hoechst-33342 ...
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bioRxiv - Biochemistry 2021Quote: ... lung or intestinal tissue were fluorescently labeled via reductive amination with 2-aminobenzamide (2-AB) (Sigma-Aldrich Canada)42 ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to PVDF, labeled with anti-HA (1:10,000, Pierce) and anti-mouse IgG-HRP (1:50,000, Sigma) and developed using ECL (GE Healthcare) ...
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bioRxiv - Epidemiology 2019Quote: ... using 90 μL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St ...
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were labeled by incubation with 1:1000 dilution of AlexaFluor488-conjugated anti-NeuN antibody (MAB377X, Millipore) and a 1:1000 dilution of Hoechst 33342 at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was labeled with mouse antiserum specific to reconstituted GFP (1:250; Sigma-Aldrich, Cat#G6539, RRID:AB_259941) [123] and either anti-serotonin (antibodies listed above).
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled RNA probes were generated using the DIG RNA Labeling Kit SP6/T7 (Sigma-Aldrich cat. # 11175025910) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Soils were amended with 150 µl of either 13C7-labeled p-hydroxybenzoic acid (99% atom 13C; Sigma-Aldrich) or unlabeled control (‘12C’ ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of cell suspension was labeled with Annexin V Alexa Fluor® 488 and Propidium Iodide (Sigma), incubated for 15 minutes at room temperature and analyzed on a BD FACSAria II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... analyzer 1×106 asynchronously growing cells were labeled for 30 minutes with 5 μg/ml Hoechst 33342 (Sigma), trypsinized ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was achieved using appropriate horseradish peroxidase-labeled secondary antibodies and Immobilon Western chemiluminescent HRP substrate (Millipore Sigma). Band intensity was determined with ImageJ 2.0.0-rc-69/1.52p software (http://imagej.nih.gov/ij ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.3% Triton) and then incubated for 1 hr 45 minutes at room temperature with Cy3-labeled phalloidin (Sigma) to label the cell contours of the epithelial cells ...
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bioRxiv - Cancer Biology 2019Quote: ... F9 and WEHI-164 tumors was studied on ice-cold acetone-fixed 8-10μm cryostat sections stained with FITC labeled IgG(L19) (FITC_F7250 Sigma) (final concentration 2μg/mL ...
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bioRxiv - Biochemistry 2019Quote: ... SILAC-labeled heavy and light samples were then mixed and allowed to bind to streptavidin-agarose beads (Novagen) for 90 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The target of interest is biotinylated either through sortagging or labeled with (+)-Biotin N-hydroxysuccinimide ester (Sigma-Aldrich). For NHS labeling ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T cells were purified from the spleen and mesenteric lymph nodes of pTreg TN/Rag1 KO mice following the manufacturer’s instructions (Miltenyi, Germany) and labeled with 2.5 μM CFSE (Sigma) or 3μM CellTrace Violet (ThermoFischer) ...
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bioRxiv - Bioengineering 2022Quote: MSC EVs from flask and bioreactor culture were labeled separately with lipid membrane PKH67 dye (Sigma-Aldrich, PKH67GL) as previously described [23] ...
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bioRxiv - Microbiology 2022Quote: ... the fixed and permeabilized cell monolayers were labeled with Phalloidin-Tetramethyl rhodamine B (Sigma-Aldrich, St. Louis, MO) for 1h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml).
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Cancer Biology 2022Quote: ... using 90 µL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St ...
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bioRxiv - Molecular Biology 2023Quote: ... Dry weight of the <3kDa WCL fractions were obtained and 30 µg of material from each of the strains and replicates were resuspended in 30 µl of loading buffer (5% acetonitrile, 0.1% formic acid in water) containing heavy labeled RF (dioxopyrimidine-13C4,15N2, Sigma) as an internal standard at a concentration of 1 ng/µl ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with the next primary antibodies: mouse-anti-α-tubulin (1:500; Sigma monoclonal DM1α), rabbit-anti-PCM1 (1:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Pathology 2023Quote: ... normal fibroblasts and MMP2-overexpressing fibroblasts were labeled with the red fluorescent dye PKH26 (MINI26-1KT, Sigma-Aldrich) according to the manufacturer’s instructions and then subcutaneously injected into cultured human skin explants at a density of 5 × 106 cells/ml in 100 μl using disposable 35G needles (ReactSystem ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The immunostained lymph node tissues were scanned using an Olympus VS-120 slide scanner with a Hamamatsu ORCA-R2 C10600 digital camera ...
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bioRxiv - Cell Biology 2023Quote: RPE-1 cells pulse-labeled with fluorescent nucleotides were incubated with 1µg/mL Hoechst 33258 (Cat.# 14530, Sigma) in 1xPBS for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... added at the time of treatments and labeled by the click reaction with Azide Fluor 545 (Sigma Aldrich) as reported (14,19) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... ctenophore cells were prepared as previously described 46,47,73 and labeled with mouse anti-histone H11-4 (EMD Millipore) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 100 pg/µL of a stable isotope labeled amino acid mixture for mass spec (Sigma Aldrich; cat# 909653) and 2-Amino-3-bromo-5-methylbenzoic acid was added to each sample and vortexed briefly ...
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bioRxiv - Physiology 2024Quote: ... Each meal was labeled with either 1% rhodamine B (absorption/emission: 540 nm/575 nm) (Sigma-Aldrich R6626) or 0.002% fluorescein (487 nm/528 nm ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...