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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Hexadecanoic acid 3 trimethylsilyl oxy methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma). Biotin-labelled probes were hybridized with sRNAs on the nylon membrane and stabilized streptavidin-horseradish peroxidase was used to detect the biotin signal.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Systems Biology 2024Quote: ... 5µM 6-bromoindirubicin-3-oxime / GSK-3 inhibitor (Sigma, 361550-1MG) and 100 µg/ml Primocin (InVivogen ...
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bioRxiv - Biophysics 2024Quote: ... 15 mM 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide (Millipore Sigma E6383), 5 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2021Quote: ... Terminal cellular differentiation was induced with 10 μM N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT; Sigma-Aldrich) while mucus-secretion was enhanced with 10 nM phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated for 16 hours with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-phenylglycin T-butyl ester (DAPT) (Sigma Aldrich), an inhibitor of γ-secretase ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligand was added to Atto550 NHS-ester in a 245 µl reaction in DMF while stirring and was then supplemented with 5 µl diisopropylethylamine (Sigma) (final concentrations ...
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bioRxiv - Bioengineering 2024Quote: ... the 3D collagen matrices were stained with 50 µM 5-(6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Sigma-Aldrich, Darmstadt, Germany) overnight at room temperature and subsequently rinsed with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... animals were injected with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (Sigma, Cat no. D5942) (10 mg/kg/day ...
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bioRxiv - Immunology 2023Quote: ... Total PBMCs (5 x 106 cells/ mL) were stained with 2.5 mM carboxyfluorescein succinimidyl ester (CFSE, Sigma #21888-25MG-F) in the dark at 4°C for 8 minutes prior to quenching with newborn calf serum ...
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bioRxiv - Bioengineering 2023Quote: ... 3D collagen matrix systems were stained with 50 µM of 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE, Sigma-Aldrich) at room temperature for 60 minutes and visualized using a confocal laser scanning microscope (cLSM ...
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bioRxiv - Genomics 2023Quote: ... 100 μg antibody in 100 μL of 100 mM NaHCO3 (pH 8.3) was conjugated with dibenzocyclooctyne (DBCO)- PEG4-NHS ester (Sigma, 764019) by adding 0.25 μL of DBCO-PEG4-NHS (dissolved at 25 mM in dimethylsulfoxide (DMSO ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then labeled with donkey anti-rabbit secondary antibodies which had been conjugated in house with azide-modified oligonucleotides with a sequence of TTATACATCTA (order No. 2023-67432, Biosynthesis) using dibenzocyclooctyne-sulfo-N-hydroxysuccinimidyl ester (762040, Sigma) as a cross-linker ...
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bioRxiv - Immunology 2024Quote: ... human memory Th cells were labelled with 0.05 mM carboxyfluorescein diacetyl succinimidyl ester (CFDASE) cell proliferation reagent (Millipore Sigma Aldrich) according to published protocol (26) ...
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bioRxiv - Bioengineering 2024Quote: ... reconstituted collagen matrices were stained with 50 µM 5-(and 6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Sigma‒Aldrich, Schnelldorf, Germany) at room temperature for 60 minutes and then washed three times with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR amplicon was digested with DpnI and desalted for 15 min by drop dialysis using a mixed cellulose ester membrane (0.025 µm size, Merck Millipore Ltd) and then used for transformation into the L ...
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bioRxiv - Microbiology 2020Quote: ... The biofilm was fixed using 25μL of Bouin solution (picric acid 0.9%, formaldehyde 9% and acetic acid 5%, HT10132, Sigma-Aldrich) for 10min ...
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bioRxiv - Immunology 2021Quote: ... and maturation was induced by adding 30ng/ml polyinosinic acid: polycytidylic acid (Poly I:C) (Sigma-Aldrich, St. Louis, MO). The maturation of DCs was assessed ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared in 1:1 mixture of H2O-ACN with 0.1% TFA by mixing an equal amount of peptide fractions (0.5 μl) with α-cyano-4-hydroxycinnamic acid (CCA)/2,5-dihydroxy benzoic acid (DHB) matrix (Sigma) and spotted on a stainless-steel plate and air-dried ...
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bioRxiv - Microbiology 2021Quote: ... The pellets were then resuspended in 300µl of cold L-Amino Acid Assay buffer according to the manufacturer instructions (L-Amino Acid Quantification kit, Sigma) and lysates were then centrifuged at 13,000 rpm for 10min at 4°C to remove insoluble material ...
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bioRxiv - Cell Biology 2021Quote: ... Free fatty acids were measured by a colorimetric assay using the Free fatty acid quantification Kit (Sigma-Aldrich, #MAK044) following manufacturer’s instructions.
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bioRxiv - Systems Biology 2022Quote: ... yeast nitrogen base without amino acids (Cat#Y0626) and glass beads (acid washed, 425–600 µm, Sigma, Cat#G8772) were purchased from Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: Purification of fatty acids was performed using the fatty acids purification kit cat MAK174 (Sigma Aldrich, St Louis, MO) from 0,15 grams of BFT flour ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was removed by careful pipetting and the biofilms were fixed using 100 µL of Bouin’s solution (picric acid 0.9%, formaldehyde 9% and acetic acid 5%, HT10132, Sigma-Aldrich) for 10min ...
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bioRxiv - Immunology 2020Quote: ... Media was gently aspirated and cells were resuspended in 75μl cell culture medium (Folic acid deficient RPMI supplemented with 1μg/ml Folic Acid (Sigma-Aldrich), 10% FCS (Low endotoxin) ...
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bioRxiv - Developmental Biology 2022Quote: ... Free (non-esterified) fatty acids were measured using Roche’s Free Fatty Acid Kit (half-micro test) (Sigma Aldrich – 11383175001), which is based on the enzymatic conversion of free fatty acids to acyl CoA by acyl-Co A synthetase.
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bioRxiv - Plant Biology 2024Quote: ... PG activity was determined by measuring the release of galacturonic acid (GalUA) residues from polygalacturonic acid (Sigma-Aldrich P3889) using GalUA as the standard (Boudart et al. ...
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ERK signaling promotes resistance to TRK kinase inhibition in NTRK fusion-driven glioma mouse modelsbioRxiv - Cancer Biology 2024Quote: ... Tartaric acid buffer stock was prepared by mixing 10 ml of 0.15 M DL-tartaric acid (Sigma Aldrich PHR1472) with 50 ml of 0.15 M tartrate sodium (Sigma Aldrich PHR1409) ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then dried and treated overnight with the Acid-pepsin solution (0.1 mg/mL pepsin in 0.5 M acetic acid, Sigma – Aldrich) at 4°C on a mechanical shaker ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was removed by careful pipetting and the biofilms were fixed using 150 µL of Bouin’s solution (picric acid 0.9%, formaldehyde 9% and acetic acid 5%, HT10132, Sigma-Aldrich) for 10min ...
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bioRxiv - Biochemistry 2023Quote: ... dried peptides were dissolved in 20 μl of 2% formic acid supplemented with 20 mM citric acid (Sigma-Aldrich). Subsequently ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Was added 150 µL of a mixture containing thiobarbituric acid (TBA, 0,5%) and trichloroacetic acid (TCA, 20%, Sigma Aldrich) in the samples (50 µg of protein) ...
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bioRxiv - Neuroscience 2024Quote: ... This second anti-CTGF antibody required an antibody retrieval process with citric acid buffer (10 mM citric acid, pH 6.0, Sigma, 251275; 0.05% Tween 20, Sigma, P1379) performed for 30 min at 95°C prior to the initial blocking step ...
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bioRxiv - Microbiology 2024Quote: Glass slide with deposited purified tissue cysts were washed thrice in 1X citric acid buffer (100mM citric acid/ 100mM dibasic sodium citrate sesquihydrate pH4.6-Sigma) in a Coplin jar ...
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bioRxiv - Neuroscience 2024Quote: ... coated cell culture plates and growth in NBM (base of neurobasal medium supplemented with nonessential amino acids, GlutaMAX, N2, B27, ascorbic acid (0.2 μg/ml, Sigma), brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cancer Biology 2020Quote: ... Each spheroid was made by resuspending 8,000 cells in 15 μl media with 0.24% methyl cellulose (4,000 cpi, Sigma-Aldrich, St. Louis, MO) by the hanging drop method 34 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287, 4-nitroquinoline 1-oxide, Sigma N8141 ...
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bioRxiv - Immunology 2021Quote: ... overnight under gentle agitation at 4°C and eluted with lectin elution buffer containing 1 M Methyl a-D-mannopyranoside (M6882, Millipore Sigma). The resulting solution was dialyzed in PBS and further purified by size exclusion chromatography using Sephacryl S-500 HR resin.
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... LMPs were cultured in this cholesterol-free medium for 20 t0 24 hrs and then treated with 10mM methyl-β-cyclodextrin (MβCD, Sigma C4555) for 15min at 37°C to remove cholesterol from plasma membranes ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Plant Biology 2021Quote: ... tomato leaflets from the 3rd leaf were detached and immediately dipped for 10 sec in a 50 μM or 100 μM methyl-jasmonate (MeJA; Sigma-Aldrich) or mock solution (0.1 % EtOH ...