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Citations for Millipore Sigma :
3101 - 3150 of 3746 citations for FGF 19 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cell were then fixed as previously described and all three batches were permeabilised with Triton X-100 0.5% in PBS and a Cy5-conjugated anti-human (AC112S, Sigma) was used for the detection of T-DM1 and T-DXd ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were seeded onto plasticware coated with a solution of 0.1 % gelatin containing 10 µg/ml human plasma fibronectin (FN) (Millipore). and collagen type 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human peripheral blood mononuclear cells (PBMCs) (FUJIFILM Wako Pure Chemical, Osaka, Japan) were cultured in RPMI1640 (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Human mini-colons cultured in biological triplicates were extracted from the PDMS chips and digested with collagenase I (Sigma) at 100 U/ml concentration in Advanced DMEM/F12 with penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Cell Biology 2024Quote: ... 3,3’ s-dihexyloxacarbocyanine iodine (DiOC6) and fibrinogen from human plasma were obtained from Sigma Aldrich (St. Louis, Missouri, USA). ML161 (PAR1 inhibitor ...
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bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
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bioRxiv - Microbiology 2024Quote: Human neutrophils from the peripheral blood of healthy blood donors were isolated by density gradient centrifugation (Histopaque; Sigma–Aldrich) followed by hypotonic lysis of erythrocytes ...
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bioRxiv - Cell Biology 2024Quote: ... and human breast cancer cells (4175-LuT; Hoshino et al., 2015) were cultured in Dulbecco’s modified Eagle’s medium (Sigma‒ Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Human GBM commercial cell lines were maintained routinely in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, New York, NY, USA), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington, MA, USA, cat# SCC507) and cultured in vendor-specified homebrew media ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were washed in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) and blocked in 1% human serum albumin (Sigma-Aldrich) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... and glucagon were measured using a customized MILLIPLEX human metabolic Hormone Panel V3 (Millipore Sigma, cat. no. HMH3-34K). CCL2 ...
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bioRxiv - Cell Biology 2024Quote: Madin-Darby canine kidney cells with stable expression of human α-2,6-sialyltransferase (MDCK-SIAT1) (Sigma, catalog #: 05071502-1VL), human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen ...
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bioRxiv - Cell Biology 2024Quote: ... human embryonic kidney HEK-293 T cells and human dermal fibroblasts (HDFs) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Microbiology 2024Quote: ... Chips were coated overnight at 4°C with ECM composed of 200 ug/mL of human Collagen IV (Sigma) + 100 ug/mL of Matrigel (Corning) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Immunology 2019Quote: ... then were incubated with both mouse anti-IgG4 and a rabbit polyclonal anti-human IL-5Rα Ab (Sigma PA5-25159) or rabbit IgG in the first step ...
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bioRxiv - Bioengineering 2019Quote: ... and subsequently incubated overnight with the Notch ligand Delta-1 (courtesy of the Bernstein Lab, FHCRC) or human IgG (Sigma) to achieve oriented immobilization ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were plated on either tissue culture plastic (TCP) or hydrogel substrates coated with 200µg/ml human plasma fibronectin (Corning, NY, USA and Millipore, Germany) at a density of 5,000 to 15,000 cells/cm2.
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bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was harvested for cytokine detection using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL fibronectin (Gibco ...
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bioRxiv - Immunology 2021Quote: Focus reduction neutralization test (FRNT) in the presence of increasing concentrations [volume/volume] of complement from normal human serum (Sigma), including 0 ...
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bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Microbiology 2020Quote: ... The porous membranes were coated on both sides with collagen type IV from human placenta (0.5 mg/mL in water; Sigma-Aldrich) at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were incubated at 30°C for 15 or 30 minutes in the presence of 750 nM DCLK1 and 0.617 μg human calpain 1 (Sigma, C6108), then analyzed by SDS-PAGE.
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...