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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 30°C (Sigma 3-16PK, Germany) to remove the non-soluble fraction of the nanocomposites ...
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bioRxiv - Molecular Biology 2024Quote: ... monothioglycerol (3 μL/mL, Sigma-Aldrich), BMP-4 (bone morphogenic protein 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... CHIR99021 (Sigma Aldrich, #SML1046, 3 µM), and Rspo3-Fc Fusion Protein Conditioned Medium (Immunoprecise ...
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bioRxiv - Bioengineering 2021Quote: ... 250 μL of recovered solution was transferred into a 96-well plate and the recovered glucose concentration was measured using a glucose (GO) assay kit purchased from Sigma (GAGO20). Glucose recovery rate was defined by the following formula.
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bioRxiv - Cell Biology 2020Quote: ... 30 μL of samples and standards were added to a 96-well plate and glucose was measured using the Glucose Oxidase (GO) Assay kits (Sigma, GAGO20). Total circulating sugars was measured from the trehalase-treated sample ...
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Microbiology 2021Quote: ... Total carbohydrate was determined by the Dubois phenol-sulfuric acid method (76) adapted to a 96-well plate assay with the Total Carbohydrate Assay Kit (MAK 104, Sigma-Aldrich), using D-glucose as the standard and absorbance read at a wavelength of 490 nm ...
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bioRxiv - Microbiology 2021Quote: ... samples were centrifuged at 10,000g for 5 min and the Acetyl-CoA measurement was performed in a 96-well plate using the Acetyl-Coenzyme A Assay Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Glycerol content for all supernatant samples was measured simultaneously in a single 96-well plate using a Glycerol Assay Kit (Sigma-Aldrich) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... GLP-1 and GIP-1 enzyme activity was done by using EIA kit (Sigma-Aldrich). DPP-4(dipeptidyl peptidase ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteoglycan content was quantified using the colorimetric dimethyl-methylene blue (DMMB) assay (Sabiston et al., 1985) by measuring the absorbance 525nm with chondroitin sulfate from bovine cartilage as standards (Sigma-Aldrich, St. Louis, MO), and then normalized to wet weight of the IVD ...
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bioRxiv - Physiology 2020Quote: Wildtype lean and obese mice were randomly divided into treatment groups and dosed daily with 8 mg of ethanolamine-O-sulfate (EOS; Sigma-Aldrich, St. Louis, MO), vigabatrin (United States Pharmacopeia ...
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bioRxiv - Bioengineering 2020Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Cancer Biology 2021Quote: ... protein samples were separated on a sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto Immobilon-P PVDF membrane (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h in blocking buffer (Tris-buffered saline ...
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bioRxiv - Bioengineering 2021Quote: ... The kidneys were then perfused via the renal artery at a 4 ml/min rate with 0.5% sodium dodecyl sulfate (Sigma-Aldrich, St. Louis, MO, USA) for a minimal perfusion time of 6 hours ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of filtered kanamycin sulfate (100 mg/mL, SERVA Electrophoresis #26899) and antifungal drug (50 μL of amphotericin B solution Sigma-Aldrich (250 μg/mL) #A2942 ...
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bioRxiv - Cancer Biology 2021Quote: ... Denatured proteins in the supernatant were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (Millipore Corporation, Billerica, MA, USA) membranes ...
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bioRxiv - Cancer Biology 2022Quote: Western blot analysis was done on a 4-12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Sigma, Immobiolon-P, Cat # IPVH20200) as previously described [1] ...
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bioRxiv - Bioengineering 2021Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Molecular Biology 2021Quote: ... Absorbance of samples were read at 600 nm and concentration of proteoglycans were measured against a standard concentration of chondroitin sulfate (Sigma-Aldrich, 39455-18-0).
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bioRxiv - Physiology 2020Quote: Wildtype lean and obese mice were randomly divided into treatment groups and dosed daily with 8 mg of ethanolamine-O-sulfate (EOS; Sigma-Aldrich, St. Louis, MO), vigabatrin (United States Pharmacopeia ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The scFv plasmid library was then transformed into EBY100 cells by the lithium acetate method82 and transformants were recovered in 100 mL molten SDCAA (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich, St. Louis, MO, #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were resolved on sodium dodecyl sulfate polyacrylamide gels under denaturing conditions and blotted onto PVDF membranes (Immobilon-P; Millipore, Burlington, MA, United States) by wet transfer for 1–2 h at 90 V ...
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bioRxiv - Cell Biology 2023Quote: ... After fixation cells were placed in hybridization buffer (5% dextran sulfate, 50% formamide, 5× SSC, 100 μg/ml heparin, 100 μg/ml sonicated salmon sperm DNA (Sigma-Aldrich, cat. No. D9156),0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Physiology 2023Quote: ... The kidneys were then perfused via the renal artery at a 4 ml/min rate with 0.5% sodium dodecyl sulfate (Sigma-Aldrich, St. Louis, MO, USA) for a minimal perfusion time of 6 hours ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were obtained from cell pellets after lysis with 100 μL to 200 μL of sodium-dodecyl-sulfate (SDS) lysis buffer (2% SDS, 10% glycerol, 50 mM of Tris HCL) plus protease inhibitor cocktail (Sigma Aldrich, Catalog. No. 11836170001). Cell lysates were centrifuged for 15 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: The synthetic media used in this study contains 6.7 g/L yeast nitrogen base with ammonium sulfate (Conda Pronadisa #1545) and 2% glucose (SD) or 0.2% oleic acid (Sigma-Aldrich, St. Louis, MO, USA) + 0.1% Tween 80 (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Pups were initially fed with 50 μL of Esbilac formula (PetAg, Hampshire, IL) with or without dextran sodium sulfate (DSS, Sigma-Aldrich, St Louis, MO) supplementation ...
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bioRxiv - Microbiology 2020Quote: ... treated 3T3 mouse fibroblast feeder cells and grown until 70% confluency in the 3:1 mixture of Ham’s F12/DMEM media (HyClone) supplemented with 5% FBS (Sigma), 24 μg/mL adenine (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of purified LDL were mixed with 50 µL DiI stock solution (3 mg/mL in DMSO; Sigma) and incubated at 37℃ for 8 h ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by selection in 1 μg/mL (C4-2B) or 1.5 μg/mL (DU145, PC-3) of puromycin (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Neuroscience 2021Quote: ... Then mice were randomly infused 10 minutes before the test with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... before 3 washes with 1 mL PBS and saturation with a 0.5% BSA solution in PBS 1X (Sigma A7888) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...