Labshake search
Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for 6 Bromo 3 4 dihydro 2H 1 benzothiin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
-
bioRxiv - Immunology 2023Quote: ... Viability was measured using MTT assay: cells were incubated for 2h in 1 mg/mL MTT solution (Sigma) in complete RPMI ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lineage-labeling was induced at indicated time points using fresh or pre-heated (65°C for 10 min) 4-Hydroxytamoxifen (4-OHT) (Sigma H7904) stock solutions in DMSO at a final concentration of 10 μM in E3 embryo medium (Felker et al. ...
-
bioRxiv - Developmental Biology 2021Quote: Recombination of Col1a2xLPCherry-Tg was induced by keeping animals in separated tanks with water containing (Z)-4-hydroxytamoxifen (4-OHT, Sigma T5648) 2 µM overnight ...
-
bioRxiv - Biochemistry 2019Quote: ... Freshly purified CsgA was filtered using a 30 kDa cut-off column at 4°C (Amicon® Ultra-4, Sigma-Aldrich) to remove insoluble protein aggregates and seeds ...
-
bioRxiv - Genetics 2020Quote: ... cells were fixed at day 4 of differentiation with 4% paraformaldehyde for 20 minutes at 4°C followed by permeabilization with 0.1% triton™X-100 (Sigma Aldrich) for 30 minutes at room temperature and blocking in 1xPBS with 1% fetal bovine serum (Gibco) ...
-
bioRxiv - Plant Biology 2021Quote: ... roots of Arabidopsis thaliana Col-0 and sr45-1 mutant seedlings (or 9-week-old plants) were vacuum infiltrated with HCl (4%, v/v) and K-Ferrocyanide (II) (4%, w/v, Sigma-Aldrich) (1/1 ...
-
bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 56 d at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were fixed overnight in a solution containing 4% formaldehyde and 4% acrylamide in PBS and adhered to a poly-L-lysine (Sigma, P4707) coated coverslip ...
-
bioRxiv - Molecular Biology 2020Quote: ... the ERS alleviator 4-phenyl butyric acid (4-PBA, 5 mM) and the ATF6 inhibitor Ceapin-A7 (500 nM) were purchased from Sigma-Aldrich. Chondrocytes were cultured in DMEM supplemented with Tm or Tg for 6 h ...
-
bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
-
bioRxiv - Cancer Biology 2021Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells in three sequential 4 h incubations in the presence of 4 μg/ml polybrene (Sigma-Aldrich). Transductants were selected in hygromycin (100 μg/ml) ...
-
bioRxiv - Neuroscience 2021Quote: ... With the fluorescent GCase substrates blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MU) (Sigma-Aldrich; as described in 11,12), PBMCs were washed 2x with PBS and lysed in 60μl of activity assay buffer (50mM Citric acid ...
-
bioRxiv - Neuroscience 2022Quote: ... where they were lightly anesthetized with isoflurane (to reduce stress from injection) and intraperitoneally injected with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) at a dose of 50 mg/kg ...
-
bioRxiv - Immunology 2022Quote: Itaconate (ITA) and its derivatives 4-octyl itaconate (4-OI) and Dimethyl itaconate (DMI) were purchased from Sigma-Aldrich (Deisenhofen, Germany) (ITA ...
-
bioRxiv - Neuroscience 2019Quote: ... ubi:Switch double transgenic fish were immersed either for ten minutes in fish water containing 0.5 μM 4-hydroxytamoxifen (4-OHT - Sigma-Aldrich, T176) for clonal recombination of the reporter construct or 7 hours per day ...
-
bioRxiv - Cell Biology 2021Quote: EMT was induced in the HTER and HSER cell lines by chronic stimulation with 4 ng/ml 4-hydroxytamoxifen (4OHT; Sigma Aldrich) for 14 days9 ...
-
bioRxiv - Biophysics 2020Quote: ... pooled and concentrated to 2-4 mg/mL using Amicon Ultra-4 (100 kDa molecular weight cut off) concentrator tubes (EMD Millipore), flash frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Neuroscience 2019Quote: ... with the following modifications: 4-day-old female adult brains were dissected in PBS and then fixed in 4% (wt/vol) paraformaldehyde (Sigma-Aldrich) in PBS on ice for 20 min ...
-
bioRxiv - Neuroscience 2020Quote: ... TrkCCreERT2::Rosa26ChR2-YFP mice were treated with a single intrathecal (i.t.) injection of 45 ng of 4-hydroxytamoxifen (4-OH Tamoxifen, Sigma Aldrich, H7904). Experiments were performed at least one week after the treatment.
-
bioRxiv - Microbiology 2020Quote: Phosphatase and lipase activity were measured by following the hydrolysis of fluorescent substrates 4-Methylumbelliferyl phosphate and 4-Methylumbelliferyl oleate (both from Sigma-Aldrich) following standard methods (Hoppe ...
-
bioRxiv - Molecular Biology 2020Quote: ... The fraction was incubated for 4 hours at 4°C with 250 μl of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
-
bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
-
bioRxiv - Biophysics 2020Quote: ... MEFs transfected with LIM-protein plasmids were plated in 4-well Strex chambers (STB-CH-4W, 2 cm2 × 4 chambers) coated with 50 μg/mL fibronectin (Millipore, FC010), and allowed to recover overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was incubated for 4 hrs at 4 °C with 250 µL of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... onto 4-inch silicon (Si) wafers previously coated with a sacrificial layer in poly(4-styrene-sulfonic acid) (PSS; 561223, Sigma Aldrich). Thiol-ene photopolymerization was performed under UV light (365 nm ...
-
bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... erythrogramma embryos were fixed for in-situ hybridization (ISH) for ∼16 hrs overnight at 4 °C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894 ...
-
bioRxiv - Immunology 2023Quote: ... The FLAG-tagged WT sCTLA-4 or Y139A sCTLA-4 in the culture supernatant was purified with the anti-FLAG M2 affinity gel (Sigma-Aldrich), washed with wash buffer (Phosphate buffered saline ...
-
bioRxiv - Molecular Biology 2023Quote: ... Newly synthesized RNA was then labelled by incubating the cells for 1h with 100 µM 4-thiouridine (4-sU, Sigma-Aldrich). RNA extraction was performed with peqGOLD TriFast Reagent (VWR ...
-
bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
-
bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
-
bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
-
bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
-
bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
-
bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
-
bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
-
bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
-
bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
-
bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
-
bioRxiv - Bioengineering 2023Quote: ... Tissue sections were then trimmed to a ∼ 6 mm x 6 mm region centered about the implant site and the tissue block was embedded in a 4% agarose gel (Sigma-Aldrich, Saint Louis, MO, USA). This tissue-gel block was glued onto the slicing platform of a vibratome (VT 1000S ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
-
Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
-
bioRxiv - Microbiology 2021Quote: ... The blocks were infiltrated with a 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma) for 1 h at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich, St. Louis, USA) for 1 h at room temperature and 1.5 h incubation with 2% OsO4 ...
-
bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
-
bioRxiv - Immunology 2019Quote: ... 4°C for 5 min and washed once with a PBS containing 1% BSA (Sigma Aldrich). The single cell suspensions used for 10X Genomics scRNAseq analysis were cleared of red blood cells by using a Red blood cell lysis buffer (eBioscience ...