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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for 4 Bromo 4' Butyl 1 1' Biphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Evolutionary Biology 2022Quote: ... erythrogramma embryos were fixed for in-situ hybridization (ISH) for ∼16 hrs overnight at 4 °C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was incubated for 4 hrs at 4 °C with 250 µL of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... onto 4-inch silicon (Si) wafers previously coated with a sacrificial layer in poly(4-styrene-sulfonic acid) (PSS; 561223, Sigma Aldrich). Thiol-ene photopolymerization was performed under UV light (365 nm ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
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bioRxiv - Neuroscience 2024Quote: ... λem 446 nm) and MUGAL break down was determined by quantifying the amount of 4-Methylumbelliferone liberated utilizing a 4-Methylumbelliferone (Sigma, M1381) standard curve ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a twenty-minute fixation period in PBS-MC with 4% paraformaldehyde (PFA, Electron Microscopy 15710) and 4% sucrose (Sigma 84100). Following fixation ...
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Immunology 2024Quote: ... IgGs were incubated for 4–5 h at 37°C in digestion buffer (4% (w/w of IgG) activated Papain from papaya latex (Sigma Aldrich), 10 x L-Cysteine ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral supernatants collected 24h post-transfection was used to infect subconfluent K19-KO cells in three sequential 4 h incubations in the presence of 4 ug/ml polybrene (Sigma-Aldrich). Transductants were selected using a culture medium containing 100 ug/ml hygromycin beginning 48h after infection ...
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bioRxiv - Cell Biology 2024Quote: ... the founder clone and its corresponding subclones from each system were pulsed with 10μM 4-thiouridine (4-SU. Sigma, cat. 4509) for 15 min (1F8 vs 1C9 ...
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bioRxiv - Immunology 2023Quote: ... ISRIB (Cat#SML0843) and tert-Butyl hydroperoxide (t-BuOOH, Cat# 458139) were obtained from Sigma-Aldrich. 4-HNE-(Cat#ab46545 ...
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bioRxiv - Immunology 2023Quote: Native IsoLG generation was induced by treating cultured cells with tert-butyl hydroperoxide (tBHP, Sigma-Adrich) at 100 μM for 30 minutes as previously described (18) ...
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bioRxiv - Biochemistry 2024Quote: ... H2O2 (hydrogen peroxide) and t-BHP (tert-butyl hydroperoxide) were purchased from Sigma-Aldrich (Steinheim, Germany) and prepared as 0.88 M and 0.078 M stock solutions respectively in distilled water at the time of use ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were extracted by adding 1250 uL tert-butyl methyl ether (HPLC grade, Sigma-Aldrich, USA) and 375 uL methanol (HPLC grade ...
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bioRxiv - Biochemistry 2024Quote: ... Lipids were extracted by adding an additional 1250 μl tert-butyl methyl ether (Sigma-Aldrich, 34875) and 75 µL methanol to each sample ...
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bioRxiv - Microbiology 2020Quote: ... Following a 15 min entry phase under standard cell culture conditions the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% PFA and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4; adenosylcobalamin or AdoCbl, Sigma, Cat #13870-90-1) and propionic acid (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with one or more of the following primary antibodies overnight at 4°C: AGO2 1:1500 dilution (Sigma-Aldrich SAB4200085 Lot#087M4841V), GW182 1:10,000 dilution (Bethyl A302-329A-T Lot#A302-329-T-2) ...
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bioRxiv - Neuroscience 2020Quote: Mice were humanely killed by pentobarbital overdose (>100 mg·kg−1) and transcardially perfused with paraformaldehyde solution (4% PFA; Sigma-Aldrich, St Louis, MO, USA). The head was removed and postfixed in PFA (4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed three times in 1× PBST prior to mounting on glass slides in glycerol with Mowiol 4-88 anti-fade agent (EMD Millipore Corp., Billerica, MA). When required ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Neuroscience 2020Quote: ... and transcardially perfused with 1 M phosphate buffer saline (PBS, 50 ml) followed by 4% paraformaldehyde (PFA, 100 ml, Sigma, St. Louis, MO, USA). Brains were removed and post-fixed in 4% PFA (50 ml ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Neuroscience 2021Quote: ... washed twice in PBS and incubated overnight at 4°C in primary anti-KCC2 raised in rabbit (1:1000, Millipore/Upstate, Cat. #07–432), anti-CGRP raised in mouse (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed and surface-stained for 30 min at 4°C in the dark and fixed with 1% of paraformaldehyde (Sigma-Aldrich, St. Louis, MO). LOQ and % responders are calculated as described above.
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bioRxiv - Immunology 2022Quote: ... cells were washed and surface stained for 30 min at 4°C in the dark and fixed with 1% of paraformaldehyde (Sigma-Aldrich, St. Louis, MO). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... catalog # BP151-500) in PBS for 1 hour followed by overnight incubation at 4°C with the γ-H2AX antibody (Millipore Sigma, catalog # 05636I) using a 1:150 dilution and the H3K9me3 antibody (Millipore Sigma ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557 ...
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bioRxiv - Developmental Biology 2020Quote: ... Standard IF staining was carried out by incubation of tissue sections with primary antibodies overnight at 4 °C at the following dilutions: mouse anti-acetylated tubulin (Sigma cat. # T7451; 1:1000), mouse anti-RAB11 (BD Transduction Laboratories cat ...
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bioRxiv - Genomics 2021Quote: ... Serum samples obtained from vaccinated and non-vaccinated individuals were stored at −20°C until tested treated with receptor destroying enzyme (RDE) (Sigma C8772, diluted 1:4), for 16 h prior to heat inactivation (30 min ...
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bioRxiv - Developmental Biology 2022Quote: Dissected intact embryos were fixed for 1 hr at room temperature with 4% paraformaldehyde (SantaCruz, Cat No. sc281692) in PBS (Sigma-Aldrich, Cat No. P4417). The embryos were then rinsed twice in PBST-0.1% (PBS with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Neuroscience 2023Quote: Mice were humanely killed by pentobarbital overdose (>100 mg·kg−1) and transcardially perfused with paraformaldehyde solution (4% PFA; Sigma-Aldrich, St Louis, MO, USA). The head was removed and postfixed in PFA (4°C ...