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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for SLAM Family Member 8 SLAMF8 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Immunology 2024Quote: ... mice were deprived of water for 4hrs and orally administered 200 ml of PBS containing 8 mg of FITC-dextran (4000 Da; FD4, Sigma Aldrich, Saint Louis, MO) per 20 g of body weight ...
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bioRxiv - Cell Biology 2023Quote: Titration of lentiviral vectors was performed by transducing HOS cells (ATCC CRL-1543) with serially diluted vector preparations in the presence Polybrene (5-8 µg/mL; Millipore Sigma, Burlington, MA, USA). HOS cells were grown in DMEM (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HEK293T cells were transduced with lentivirus encoding the dGFP reporter in the presence of 8 µg/ml polybrene (Millipore Sigma TR-1003-G). Three days after transduction ...
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bioRxiv - Plant Biology 2023Quote: ... One-liter cultures were centrifuged and resuspended in Buffer A (50 mM Tris pH 8, 200 mM NaCl) with protease inhibitor cocktail (Sigma, St. Louis, MO, USA), DNase I (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... HIV infections were established via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL polybrene (Millipore Sigma #TR-1003-G) as previously described18 ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Genomics 2023Quote: ... The seeded cells were transduced with the viral library by supplementing their media with 8 μg/mL of polybrene (Sigma-Aldrich Inc # TR-1003) and adding a variety of viral volumes ranging from 0 μL to 50 μL prior to centrifugation at 1000 g for 2 hours at 33°C ...
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bioRxiv - Neuroscience 2023Quote: ... striatal neurons were plated at a density of 50K per well onto 24 well plates or 20K per well onto 8 well chamber slides coated with poly-L-lysine (Sigma Aldrich, St. Louis, USA) (100 µg/ml) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we added an equal volume of stop solution (8 M urea, 1 M Tris–HCl [pH 8.0], and a small amount of Blue Dextran [Sigma Chemical, St. Louis, MO, USA]) to stop the reactions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: Transduction of MIA PaCa-2 cells using lentiviruses was done in 1:1 ratio of viral supernatant to media and supplemented with 8 µg/ml Polybrene (Sigma-Aldrich #TR-1003-G). Following an overnight incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Head and neck cancer cell lines were incubated overnight with media containing lentiviruses supplemented with 8 µg/ml of polybrene (Millipore-Sigma, no. TR-1003-G). Transduced cells were selected with sequential treatment of puromycin (Biogems ...
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bioRxiv - Cell Biology 2023Quote: ... We lysed each pellet in 1% SDS lysis buffer: 1% SDS in 50 mM Tris (pH 8) containing protease inhibitor cocktail (Sigma Aldrich, catalog no. P8849) and 1 mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA, #IPVH00010). The membrane was incubated at 4°C overnight with the following primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 20 mL NP buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8) with 100 µl 10X Protease Inhibitor Cocktail (Sigma-Aldrich, Merck, Darmstadt, Germany). Resuspended cells were filtered with Miracloth (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cell cultures grown in 96 well plates were washed with PBS and incubated with a staining solution (5nM TMRM (Sigma-Aldrich #115532-50-8) and Hoechst were diluted in growth media and incubated for 45 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Other antibodies and probes used for western blot were as follows: anti-DHFR antibody (Sigma-Aldrich D1067), anti-HSP-6 antibody (Enzo Life Science ADI-SPS-825) ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used were as follows: anti-FLAG antibody (F3165, Sigma, diluted to 0.8 μg/ml); anti-H3K4me3 antibody (07-473 ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with the following primary antibodies: mouse anti-flag antibody 1:2000 (F1804 Sigma), mouse anti-beta-actin 1:5000 (A2228 Sigma).
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bioRxiv - Biophysics 2021Quote: ... primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma, catalogue no. SAB4600186). On-chip cell imaging was carried on with a long-distance 25x objective (NA=1.1 ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated in NGF deficient media containing a neutralizing antibody (1μg/mL anti NGF antibody, Millipore) for 12 hours ...
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bioRxiv - Microbiology 2019Quote: ... polyclonal antibodies mentioned above and secondary antibody conjugated to goat anti-Rabbit IgG conjugated alkaline phosphatase (Sigma) and detected with NBT/BCIP (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Bound antibodies were detected with Peroxidase conjugated goat anti-human kappa light chains antibody (A7164-1ML, Sigma). 100 ul TMB(Tetramethylbenzidine,Biopanda TMB-S-001 ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with the following primary antibodies overnight: mouse anti-FLAG M2 antibody (1:10,000; Sigma-Aldrich; F3165) for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were labeled first with primary antibodies and then fluorescently-labeled secondary antibodies (EMD Millipore AB16901) and DAPI (Thermo Fisher Scientific D3571) ...
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bioRxiv - Developmental Biology 2020Quote: ... conjugated with 5 μg of one of the following antibodies: mouse monoclonal antibody to Flag (Sigma, F1804) or rabbit polyclonal antibody to HA (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was then incubated in secondary biotinylated rabbit antibody to goat antibody (1:250, Sigma, B-7024) for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with the primary antibody (rabbit polyclonal anti-TBR1 antibody, 1:200, Millipore, #AB10554) overnight at 4°C in the same blocking solution reducing the GS to 5% ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was incubated with secondary antibody (1:10000) anti-Goat IgG secondary antibody (A0545, Sigma Aldrich) in the buffer (10ml 1x TBST ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used as primary antibodies: mouse monoclonal anti-Flag (M2 clone, 1:5000; Sigma), mouse monoclonal anti-6xHis (1:2500 ...
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bioRxiv - Neuroscience 2021Quote: ... the antibody produced identical patterns in the mouse retina whole mount as a different Cx43 antibody (Sigma, #C6219 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were incubated in primary antibody (mouse anti-TH antibody, 1:2000, cat#: MAB318, Sigma-Aldrich, St ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies (rabbit anti-ASNS – HPA029318, Atlas Antibodies, mouse anti-β actin, Clone AC-15, Sigma Aldrich) were diluted in LI-COR blocking buffer and detected using goat anti-rabbit-AF680 and goat anti-mouse-AF790 secondary reagents (Molecular Probes) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting was carried out using either anti-CaSR antibody (ImmunoGenes) or anti-Tubulin antibody (T5168, Sigma-Aldrich). HRP-conjugated anti-rabbit (111-035-003 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunofluorescence were performed with the following antibodies: mouse anti-Flag monoclonal antibody (F1804, Sigma Aldrich, 1:500); rabbit anti-G3BP1 antibody (1F1 ...
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bioRxiv - Molecular Biology 2021Quote: Immunoblotting was performed using the following antibodies: mouse anti-Flag monoclonal antibody (F1804, Sigma Aldrich, 1:2000); rabbit anti-SARS-CoV-2 N antibody (9103 ...
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bioRxiv - Microbiology 2020Quote: ... or 1/200 anti-RpsF (Dundee Cell Products) antibodies and 1/6000 anti-rabbit secondary antibody (Sigma). Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Anti-FLAG antibody and Anti-FLAG antibody-conjugated agarose beads were obtained from Sigma (St. Louis, MO). 1,1’-bi(4-anilino ...
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bioRxiv - Neuroscience 2019Quote: ... The following antibodies were used: rabbit polyclonal anti-CCK antibody (1:500, Sigma-Aldrich Cat# C2581, RRID:AB_258806) mixed either with a mouse monoclonal anti-KChiP1 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: Rabbit polyclonal antibody and mouse monoclonal antibody against FLAG were purchased from Sigma-Aldrich (St. Louis, MO). Monoclonal anti-Myc antibody was purchased from Santa Cruz Biotechnology (Santa Cruz ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for Western blot experiments: ANTI-FLAG M2 antibody (F1804 Sigma-Aldrich); Anti-RPS6 antibody (ab40820) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies and their dilutions were as follows: anti-phospho-ubiquitin S65 rabbit antibody (1:200; Millipore) and anti-βIII tubulin mouse (2G10 ...
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bioRxiv - Synthetic Biology 2023Quote: ... a 1:10000 secondary antibody solution was made by diluting polyclonal anti-mouse antibodies (Sigma Aldrich, A4416) in TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used in this study are rabbit anti full-length firefly luciferase antibodies (Sigma-Aldrich), which react with both the N-terminal and C-terminal firefly LUC fragments ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: Mouse Anti-NeuN Antibody (Millipore, Burlington MA, MAB377, dilution 1:100), Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich ...