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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... human HEK 293T embryonic kidney cells (Intercell, AG) and mouse MEFs embryonic fibroblasts Pml−/− (from Dr. Lallemand-Breitenbach) were cultivated in DMEM medium (Sigma-Aldrich, D6429) containing 10% of fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: Formalin-fixed and paraffin-embedded lung sections from mock and IAV-infected mice were prepared as previously described (47) and probed for epithelial or endothelial cells detection using monoclonal mouse anti-αSMA (1:10,000; A2547; Sigma-Aldrich, Darmstadt, Germany), rabbit anti-mouse CD31 (1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse macrophages J774A.1 cell line [NIHS (JCRB) 9108] were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma Aldrich #D-5030, USA) with 10% fetal calf serum and penicillin and streptomycin (Sigma Aldrich #F-7524 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Immunology 2024Quote: ... and TNF-α) were measured relative to unstimulated cells in supernatants using a Milliplex MAP Magnetic Mouse Cytokine/Chemokine multiplex immunoassay (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... For immunolabeling of Cajal-Retzius like cells the following antibodies were used at a dilution of 1:200: Reelin (mouse, Sigma-Aldrich, MAB5366), and Mitochondria (mouse ...
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bioRxiv - Cell Biology 2023Quote: ... sections were stained for ciliated cells (rabbit anti-RFX3, 1:100, Cat# HPA035689,Sigma-Aldrich, and mouse anti-AceTUB, 1:100, Cat# T6793, Sigma-Aldrich), club cells (rabbit anti-SCGB1A1 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse anti-Gapdh (clone GAPDH-71.1, cat# G8795) and mouse anti-MBNL1 (clone 3A4) were from Sigma. Rabbit anti-Tubulin (cat# 2148) ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... 2% Mouse-on-mouse (M.O.M) blocking reagent and 0.4% Triton X-100 (Sigma-Aldrich, St. Louis, MO). Sections were then incubated in TBS containing 10% goat (or donkey ...
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bioRxiv - Cell Biology 2019Quote: ... The CRP concentration in mouse serum was measured using the mouse CRP ELISA kit (Sigma-Aldrich, RAB1121) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2019Quote: Mouse serum insulin levels were determined using the mouse metabolic hormone panel (Millipore Sigma, Milliplex MMHMAG-44K) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2024Quote: ... then incubated with goat anti-mouse secondary HRP antibody (Sigma-Aldrich, #A0168, 1:10,000, for mouse plasma) or goat anti-human secondary HRP antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Mouse IZ-actin (Clone AC-74) and mouse FLAG M2 F3165-1MG were obtained from Sigma-Aldrich and used at 1:10 000 and 1:2 000 dilutions in western blot ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody (Mouse Anti-Influenza A NP, Millipore MAB8251 or Mouse Anti-Influenza B NP, Millipore MAB8661) was diluted 1:4000 in antibody diluent ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-α-tubulin (1:1000; CST3873) and mouse anti-IDH1 (R132H) (1:500, SAB4200548, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated overnight with primary antibodies (rabbit phospho-AURKA Thr288, 1:50; mouse anti-γ-tubulin GTU-88, Sigma-Aldrich; 1:1000) diluted in blocking buffer at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2020Quote: ... Densitometry was performed as previous described.19 Proximity ligations between ephrinb2/EphB4 in flow-aligned cells were conducted by using Duolink® In situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, MO). Numbers of individual ligations in raw and post-processed images were compared to test the validity of the quantification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... rabbit polyclonal anti-p[Thr202]ERK44/p[Thr204]ERK42 (1:10,000; product # 9101, Cell Signalling Technology) or mouse monoclonal anti-α-tubulin (1:50,000; product # T5168, Sigma-Aldrich, Oakville, Ontario, Canada). Membranes were then rinsed 4 times for 5 minutes with 0.1 % Tween 20/tris-buffered saline at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Cancer Biology 2024Quote: ... established from a Tyr:CreER;BrafCA;Ptenlox/lox mouse.(32,33) Cells were incubated at 5% CO2 at 37°C and cultured in DMEM-high glucose medium (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum and 1% penicillin and streptomycin ...
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bioRxiv - Genetics 2021Quote: ... mice were treated with E2 (6.7 ng per mouse) and P4 (P0130, Sigma-Aldrich; 1 mg per mouse) for 3 days ...