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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for Recombinant Mouse Fgfr4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mouse anti GAPDH (Sigma, 1:25,000), mouse anti p62 (BD Bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... using mouse anti-HA (Sigma- Aldrich), mouse anti-His (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Nestin (mouse, 1:400, Millipore), anti-NeuN (rabbit ...
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bioRxiv - Microbiology 2024Quote: ... or goat anti-mouse IgG (Sigma) horseradish peroxidase conjugates used at the manufacturers recommended dilution.
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-FLAG (F1804, Sigma-Aldrich) used at 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-GluA2 (MAB397, Millipore, MA), rabbit anti-Rac1 (05-389 ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-FLAG (Sigma-Aldrich, F1804), Streptavidin-HRP (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and mouse anti-α-tubulin (Sigma). Secondary antibodies used in immunoblot analysis were horseradish peroxidase-conjugated (HRP)-conjugated goat anti-rabbit IgG and HRP-conjugated rabbit anti-mouse IgG (Jackson ImmunoResearch).
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bioRxiv - Neuroscience 2024Quote: ... NF-200 (mouse, Sigma #NO-142), VAMP2 (mouse ...
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bioRxiv - Neuroscience 2024Quote: ... Ku80 (made in mouse: MAB1281, Sigma; or made in rabbit MA5-14953 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-MAD1 (Merck Millipore MABE867) was used at 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-actin (Sigma Aldrich A3853) was used at 1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-myc (1:100, Sigma), mouse anti-SAG1 (1:50 ...
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bioRxiv - Microbiology 2024Quote: Mouse anti-HA (1: 5000, Sigma), mouse anti-EF1α (1 ...
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bioRxiv - Immunology 2024Quote: ... and 5% mouse serum (Sigma-Aldrich)] was added ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-NeuN (Millipore Sigma #MAB377), rat anti-mCherry (Thermo Fisher #M11217) ...
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bioRxiv - Genomics 2024Quote: ... A mouse anti-FLAG antibody (Sigma) was used to detect spike protein expression.
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO). Microglia were isolated using anti-Cd11b beads (130-049-601 ...
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bioRxiv - Cancer Biology 2019Quote: ... Gartner’s medium consisted of alpha-MEM (BioWhittaker) supplemented with 12.5% heat-inactivated FCS and 12.5% heat-inactivated Horse serum (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in an incubator at 37°C and 5% CO2 with 100% humidity in a 1:1 mixture of EGM-2 with M199 (Thermo Fischer, 31150022) supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich, F7524-500ML), 20 µg/ml gentamicin (Thermo Fischer ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse anti-Gapdh (clone GAPDH-71.1, cat# G8795) and mouse anti-MBNL1 (clone 3A4) were from Sigma. Rabbit anti-Tubulin (cat# 2148) ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... 2% Mouse-on-mouse (M.O.M) blocking reagent and 0.4% Triton X-100 (Sigma-Aldrich, St. Louis, MO). Sections were then incubated in TBS containing 10% goat (or donkey ...
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bioRxiv - Cell Biology 2019Quote: ... The CRP concentration in mouse serum was measured using the mouse CRP ELISA kit (Sigma-Aldrich, RAB1121) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2019Quote: Mouse serum insulin levels were determined using the mouse metabolic hormone panel (Millipore Sigma, Milliplex MMHMAG-44K) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2024Quote: ... then incubated with goat anti-mouse secondary HRP antibody (Sigma-Aldrich, #A0168, 1:10,000, for mouse plasma) or goat anti-human secondary HRP antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Mouse IZ-actin (Clone AC-74) and mouse FLAG M2 F3165-1MG were obtained from Sigma-Aldrich and used at 1:10 000 and 1:2 000 dilutions in western blot ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody (Mouse Anti-Influenza A NP, Millipore MAB8251 or Mouse Anti-Influenza B NP, Millipore MAB8661) was diluted 1:4000 in antibody diluent ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-α-tubulin (1:1000; CST3873) and mouse anti-IDH1 (R132H) (1:500, SAB4200548, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...