Labshake search
Citations for Millipore Sigma :
3051 - 3100 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: Equal amounts of protein samples were supplemented with β-mercaptoethanol (Sigma; M6250) and diluted in 1X Laemmli Sample buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... Eluted protein was concentrated using spin concentrators (Millipore Amicon Ultra-15 -3K) to ∼1.5-2 mL and subsequently dialyzed in one liter of storage buffer (150 mM NaCl ...
-
bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins in the SDS gels were transferred to PVDF (Cat# IPVH00010, Millipore) membrane using an electroblotting apparatus (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Bioengineering 2024Quote: ... the protein was concentrated using Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cut off of 10 kDa.
-
bioRxiv - Cell Biology 2024Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins from gels were transferred on to polyvinylidene difluoride membrane (Millipore, #IPVH00010) at 30 V overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in PBS and incubated at 4°C overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in each sample was measured using BCA assay (Sigma-Aldrich). 30µg of each sample were then prepared using the iST-NHS kit (Preomics) ...
-
bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2024Quote: ... was dissolved into 5 mL of BugBuster™ protein extraction reagent (Sigma). Using a sterile loop ...
-
bioRxiv - Cell Biology 2024Quote: ... Ice-cold protein extraction lysis buffer was composed of RIPA (Sigma, R0278), Protease inhibitor cocktail (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... and proteins were concentrated using an Amicon ® Ultra Centrifugal Filter (Millipore) with a 30 kDa cutoff ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 5 % skimmed milk in TTBS buffer (10 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
-
bioRxiv - Biophysics 2024Quote: ... The digested protein samples were then concentrated with an Amicon Ultra (Millipore) concentrator (50 kDa MWCO ...
-
bioRxiv - Cancer Biology 2024Quote: ... the protein was transferred to 0.45μm PVDF membranes (Millipore Sigma, Cat#IPVH00010) with transfer buffer (formula ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto a 0.45-μm polyvinylidene difluoride membrane (Millipore). Next ...
-
bioRxiv - Cell Biology 2024Quote: Cellular protein was collected in RIPA buffer (R0278, Millipore Sigma, Burlington, MA) containing ReadyShield® Protease and Phosphatase Inhibitor Cocktail (PPC- 2020 ...
-
The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... 50 µL of agarose beads coupled to protein A (Millipore 16–157) or G (Millipore 16–201 ...
-
bioRxiv - Immunology 2024Quote: ... were coupled to 30 μl Protein G Sepharose™ beads (#P3296, Millipore) by 2 h incubation at 4°C with rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Millipore, IPVH100010). Membranes were blocked for at least 1 h with 5% non-fat dry milk and 3% BSA in TBS-T (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein-containing factions were concentrated with Amicon Ultra Filter Devices (Millipore, USA) 10 kDa cutoff ...
-
bioRxiv - Biochemistry 2024Quote: ... The separated proteins were then transferred to a PVDF membrane (Millipore, IPVH00010) and immunodetection was carried out using antibodies against the target proteins ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using anti-FLAG (Sigma-Aldrich, ST. Louis, MO, USA) and HRP-labeled anti-mouse IgG (Cytiva ...
-
bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were then blocked and probed with anti-Drp1 (1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Exogenous protein expression was induced 24h after transfection by adding Doxycycline (Sigma) to the media at a concentration of 50 ng/ml and induction was performed for 24h ...
-
bioRxiv - Genetics 2024Quote: ... Protein was transferred to a methanol-treated polyvinylidene difluoride membrane (Millipore Sigma) using a Mini Trans Blot Electrophoretic Transfer Cell (Biorad ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a BCA assay kit (#QPBCA, Sigma-Aldrich). Protein samples were mixed with DTT (#D9779-10G ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in the collected fractions were measured using Bradford reagent (Sigma).
-
bioRxiv - Pathology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck Millipore), blocked with 5% non-fat dry milk ...
-
bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
-
bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Supernatants containing chromatin fragments were pre-cleared with protein A agarose beads (Millipore) for 20 min and incubated with a specific antibody overnight at 4 °C on a nutator (antibody information were listed in Supplementary Table 1).then added 50 ul of protein A agarose beads and incubated for 2 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... Completeness of protein transfer was determined by Ponceau S (Sigma-Aldrich, cat# P7170) staining for 5-6 min followed by frequent washing with H20 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma) or GFP (Clonetech ...
-
bioRxiv - Cell Biology 2019Quote: ... Total extracted proteins (20 μg) were derivatized according to the manufacturer’s instructions (Millipore). 2-mercaptoethanol was used to reduce samples ...
-
bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
-
bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteins were then transferred to Immobilon-P transfer membranes (PVDFs) (IPVH00010; Millipore). The membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
-
bioRxiv - Cell Biology 2020Quote: Primary antibodies against the following proteins were used: actin (rabbit, Sigma-Aldrich, #A2066), cMyc (mouse ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis.
-
bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
-
bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
-
bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
-
bioRxiv - Microbiology 2019Quote: ... inner-membrane proteins were solubilized using 0.5% (w/v) N-lauryl sarcosine (Sigma) in a buffer containing 20 mM Tris-HCl [pH 7.5] and 150 Mm NaCl for 1.5 h at 4 °C on a roller ...