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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... All images were normalized total protein from the same membrane stained using Amido Black total protein-stained membrane (A8181, Sigma).
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bioRxiv - Plant Biology 2024Quote: ... Protein STOP1 was selectively enriched from the total protein extract using Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Immunology 2024Quote: ... The eluted proteins were then dialysed in PBS and endotoxin removed by running the protein solution through Polymyxin B agarose column (Sigma), which was verified by performing a LAL test (Thermofisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Two µg of antibody was incubated for 30 min at RT with 20 μl of washed protein G-agarose beads or protein A/G mix magnetic beads (Cat. No. LSKMAGAG02, Millipore) or with protein G Sepharose 4 fast flow slurry (GE Healthcare #17061801 ...
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bioRxiv - Molecular Biology 2024Quote: Endotoxin contaminants associated with the purified protein fraction were further removed by treating the protein solution with Polymyxin B Agarose beads (P1411, Sigma), as discussed in previously published articles54–59 ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 30 k; Millipore), diluted to final concentration of 2.5mg/mL using storage buffer (20mM HEPES ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated with primary antibodies (mouse anti-PAR, 1:400 Merck Millipore Cat#AM80 RRID: AB_2155072; mouse anti-γH2AX pS139, 1:2000 Merck Millipore Cat#05-636 RRID ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunoblotted with rabbit monoclonal anti-mouse caveolin-1 and rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore) as a loading control and a marker for PM fraction ...
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bioRxiv - Cell Biology 2022Quote: ... used for HA::SYP-1) or mouse anti-HA (SYP-4) and mouse anti-α-tubulin (DM1A, EMD Millipore), each diluted 1:5,000 ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Immunology 2023Quote: ... the bound antibody was incubated with horseradish-peroxidase labelled subclass-specific anti-mouse IgG1 at 1:8,000 or anti-mouse IgG2c at 1:2,000 (Sigma, Aldrich). After five washes with PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were concentrated with Centricon filters (Merck-Millipore) when needed and/or diluted to a final concentration of 1 mg/mL in PBS.
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Cancer Biology 2021Quote: ... and proteins were transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk or 5% BSA in TBST (Tris-buffered saline containing Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Sigma) and incubated in 5% milk powder in PBS 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Immunology 2021Quote: ... affinity purified by protein A (HiTrap, Sigma-Aldrich), and tested for endotoxin levels (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-Protein A (Sigma-Aldrich, cat. # P2921) were used ...
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bioRxiv - Immunology 2021Quote: ... and proteins were concentrated using 10kDa concentrators (Millipore).
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bioRxiv - Genetics 2021Quote: ... proteins were transferred to PVDF membranes (Immobilon, Millipore) and blocked in TBS containing 5% skim milk and 0.1% Tween-20 ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were transferred to PVDF membrane (Millipore) and incubated with blocking buffer (4 % skim milk in 1 × PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were concentrated using Vivaspin columns (SIGMA) and quantified using the Bradford assay (SIGMA) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transfered to a PVDF membrane (Millipore) at 23 volts for 0.5 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured with BCA (Sigma-Aldrich), according to manufacture instructions ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using protein lysis buffer (Sigma) with protease and phosphatase inhibitor cocktails (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Monoclonal IgG directed against UBE3A protein (E8655, Sigma) and anti-β-ACTIN monoclonal antibody (A5316 ...
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bioRxiv - Molecular Biology 2021Quote: ... then pre-cleared with protein G beads (Sigma). Protein lysates were incubated with either anti c-MYC agarose beads (Sigma A7470) ...
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bioRxiv - Molecular Biology 2020Quote: ... protein extraction was performed using RIPA buffer (Sigma) supplemented with protease/phosphatase inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Molecular Biology 2020Quote: Protein extraction using trichloroacetic acid (TCA, Sigma T0699) and western blotting methods are similar to those described in (12) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a PVDF membrane (Millipore), which was then blocked in 5% milk in PBS + 0.2% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Protein was transferred onto activated PVDF membrane (Millipore) using trans-blot Turbo system (Biorad) ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were concentrated by ultrafiltration (Amicon 30K, Millipore). The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... 500 U/mL LIF Protein (Millipore Sigma ESG1106), 3 µM CHIR99021/GSK-3 Inhibitor (Millipore Sigma 361571) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein extracts were prepared using BugBuster (Millipore Sigma) diluted in binding buffer (20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were subjected to HisBind Resin (Novagen) chromatography ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated with DTT (Sigma) and IAA (Sigma ...
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bioRxiv - Immunology 2021Quote: Proteins were blotted onto a PVDF membrane (Millipore) for 1,5 h at 35 V in NuPAGE transfer buffer (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were reduced with 5 mM dithiothreitol (Sigma) for 1hr at room temperature and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were immunoprecipitated using protein G (Sigma-Aldrich) beads coated with unconjugated anti-biotin antibody (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells for protein expression (Novagen, chloramphenicol resistance).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Millipore) using a Trans-Blot® Turbo™ Transfer Starter System ...
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bioRxiv - Biophysics 2021Quote: ... Protein was concentrated using Amicon Ultra concentrators (Millipore) and flash frozen in liquid nitrogen and stored at -80°C.
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to PVDF membranes (Millipore) with 350 mA for 2 h (Mini Trans-Blot Cell ...