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Citations for Millipore Sigma :
3001 - 3050 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... E-cadherin (R&D AF748; 1:300) and FLAG-M2 (Sigma, F1804; 1:1,000). Secondary antibodies were used as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Transcription was blocked with 5 µg/ml of actinomycin D (ActD, A9415, Sigma-Aldrich). Cells were incubated with ActD for 0 ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts seeded at 30×103 cells/well on poly-D-lysine (Sigma-Aldrich, #P6407) coated 8-well microscope slides (Ibidi ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was then pre-cleared for 1 h with 25 μL of IgG agarose beads (Sigma) at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... and protease inhibitor tablet [Roche]) and immunoprecipitated for 2 h using Flag M2 affinity gel (Sigma-Aldrich). Immunoprecipitates were then extensively washed in immunoprecipitation wash buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... after LPS-priming macrophages were treated for 6 h in OptiMEM with 1.5 µM nigericin (Sigma-Aldrich) or 1 µg/ml Clostridium difficile toxin B (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... Mice in the treatment group received 2-hydroxypropyl-β-cyclodextrin powder (Sigma-Aldrich, Cat. No. H-107) dissolved in sterile phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with FAC for 24 h followed by 50 μM deferoxamine (DFO) (D9533; Sigma-Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... After incubation for 1 h with horseradish peroxidase-conjugated anti-mouse secondary antibodies (1:10,000 dilution; Millipore). Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Plates were then washed with PBS-T and incubated for 1 h with HRP-conjugated avidin (Sigma, 25 µL of a 1:3,500 dilution in blocking buffer) ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated for two h prior to stimulation with the AhR inhibitor CH-223191 (Sigma-Aldrich) at a concentration of 1μM/mL ...
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bioRxiv - Cell Biology 2021Quote: γ-secretase inhibitors used in this study were as follows: avagacestat (10 μM, 24 h treatment time, Sigma), N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester (DAPT ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich). E-buffer (20 mM Na·HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... 48 h and day 21 hBMMSC-seeded scaffolds (n=3) were fixed in 4% paraformaldehyde (Sigma, Canada) for 1 hour and submerged in gradient sucrose solutions from 10% to 30% ...
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bioRxiv - Microbiology 2020Quote: ... the medium was replaced after 1 h with fresh medium containing 100 µg mL-1 Gentamicin (Sigma) to kill extracellular bacteria ...
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bioRxiv - Microbiology 2020Quote: ... ring stage parasites (4 h post-invasion) were synchronised with sorbitol and 0.05% DMSO or RAP (Sigma, 100 nM in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... and Na+ was removed by passage over a 1-ml Dowex 50W-XB (H+ form, Sigma-Aldrich) column equilibrated with 5% acetic acid ...
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bioRxiv - Plant Biology 2020Quote: ... for 1 h at room temperature in a solution of 5 % (w/v) BSA (Sigma-Aldrich, Japan) in TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... After 2.5 h cells were additionally treated with the synthesis blocker cycloheximide (10 µg/mL, Sigma-Aldrich). Both inhibitors were diluted in phenol red-free cell culture medium (#21063-029 ...
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bioRxiv - Neuroscience 2020Quote: ... Retinal sections were incubated for 72 h with diluted antisera (anti-PKC, 1:400, mouse monoclonal, Sigma). After rinsing ...
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bioRxiv - Microbiology 2021Quote: ... Lung paraffin sections were stained with Hematoxylin and Eosin (H&E) or Gomori’s Methenamine silver (GMS) (Sigma) for pathological examination ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked for 1 h with Tris-buffered saline (TBS) containing 0.1 % Tween 20 (Sigma Aldrich) and 5 % dry milk and further incubated for 1 h with primary antibodies diluted in TBS ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution; Millipore). Detection was performed using the SuperSignal West Femto Detection Kit for anti-GFP ...
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bioRxiv - Microbiology 2021Quote: ... 4.6 ml of 48 h viral supernatant mixed with 14.1 μl of 8 μg/ml polybrene (Sigma) was added to 1.4-2.8 x106 ECs cultured to 20-30 % confluence in a T75 flask ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were either pre-incubated for 2 h with inhibitor (50 mM ammonium chloride [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transfected using polyethyleneimine (Sigma-Aldrich, Poland) with the lentiviral packaging plasmid (psPAX) ...
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bioRxiv - Molecular Biology 2021Quote: ... Culture medium was changed 6 h after transfection and supplemented with 10 μM 9-cis-retinal (Sigma) for experiments with light-activated receptors ...
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bioRxiv - Molecular Biology 2021Quote: ... Culture medium was changed 6 h after transfection and supplemented with 10 μM 9-cis-retinal (Sigma) for experiments with light-activated receptors.
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bioRxiv - Cell Biology 2021Quote: ... Supernatant was harvested 40 h after transfection followed by centrifugation and filtration (0.45 µM; SLHV033RS, Merck Millipore) to remove cellular debris ...
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bioRxiv - Immunology 2022Quote: ... and longitudinal sections were stained with H&E and Oil Red O (Millipore Sigma; Cat No O0625) to visualize BM adiposity ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h at room temperature and revealed by an enhanced chemiluminescent detection method (Immubilon Western, Millipore). Protein-loading control was performed with GAPDH or β-actin Ab.
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bioRxiv - Neuroscience 2022Quote: ... Skin sections were processed for morphometric analysis using hematoxylin and eosin (H&E) (Sigma-Aldrich, #MHS32-1L) and immunofluorescence staining (e.g. ...
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bioRxiv - Immunology 2022Quote: ... paraffin embedded and sections (5 µm) prepared and stained with hematoxylin and eosin (H&E; Sigma-Aldrich). For clinical scoring 4-6 random field of views at 10x magnification from three sections 500 µm apart were assessed per colon ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μm sections of paraffin-embedded samples were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), periodic acid-Schiff (PAS ...
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bioRxiv - Cell Biology 2022Quote: ... or for 3 h either with 25 µl of M2 anti-FLAG affinity gel (A2220, Sigma-Aldrich) or RFP-Trap Agarose (rta-10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and residual sites on the surface were blocked for 1 h with 0.25% porcine gelatin (Sigma-Aldrich) in TRIS buffer (50 mM Tris ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... treatment of cells with 2 h of 10 μM of Carbonyl Cyanide Chlorophenylhydrazone (CCCP; Sigma-Aldrich, C2759) served as a positive control due to its known ability to severely induce mitochondrial fragmentation.
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bioRxiv - Biophysics 2020Quote: ... for 1 hour at room temperature and nuclei with 1.5 µl/ml bisBenzimide H 33342 trihydrochloride (Sigma) for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed twice with buffer H containing 20 mM imidazole (Sigma-Aldrich, Cat. No. I2399) and twice with buffer H containing 40 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... and Cobalt(II) chloride hexahydrate (CoCl2, final conc. was 200 μM for 5 h; C8661, Sigma-Aldrich). Following fixation with 3.7% formaldehyde in PBS for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then washed and stained in 1 × PBS containing 50 µg/ml RNase H (Sigma Aldrich) and 50 µg/ml propidium iodide (PI ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were then washed for 3 × 1 h in 1x PBS with 0.02% NaN3 (Sigma Aldrich – 58032) at RT ...
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bioRxiv - Immunology 2022Quote: ... An anti-hamster IgG (H+L)-Alkaline Phosphatase antibody produced in goat (Sigma-Aldrich, St Louis, MO) diluted to 1:1000 in 3% BAS-PBS was added at 60 µl/well for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... an H-medium-based reaction solution was used containing horse radish peroxidase (Sigma-Aldrich, Burlington, MA, USA) and Amplex Red (Synchem ...
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bioRxiv - Cell Biology 2020Quote: Dopamine hydrochloride (#H-8502) and angiotensin II (#A9525) were purchased from Sigma-Aldrich (St. Louis, MO, USA); sulpiride (#0895) ...
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bioRxiv - Cell Biology 2021Quote: ... the cell lysates were incubated for 4 h with anti-FLAG agarose affinity gel (Sigma, A-2220). To compensate for expression differences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The culture media was replaced 12-16 h after transfection with fresh DMEM High Glucose (Sigma– Aldrich) supplemented with 10% FBS ...
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bioRxiv - Genetics 2020Quote: ... for 2 h to overnight at 37°C and stained with propidium iodide (Sigma-Aldrich, St. Louis) in the dark at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... brasiliensis yeast cells (105/ml) was performed for 2 h at 37 °C in BHI medium (Sigma). RNA isolation ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transfected using polyethyleneimine (Sigma-Aldrich, Poland) with the lentiviral packaging plasmid (psPAX) ...