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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Mouse Anti SARS Coronavirus Nucleoprotein Antibody 3861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Zoology 2019Quote: ... animals were preincubated for 30 min at room temperature in PTx+NGS (5% Normal Goat Serum in PBS + 0.1% Triton X-100) and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed 3X with tris-buffered saline with 0.05% Tween-20 (TBST) and incubated with anti-mouse secondary antibody (Sigma) at 1:50,000 for 1 hour at RT ...
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bioRxiv - Immunology 2020Quote: ... ROP5-His6-3xFlag complementation strains were assessed by IFA with a mouse anti-Flag primary antibody (Sigma, clone M2) at 1:500 dilution and Alexa Flour 594 goat anti-mouse IgG secondary antibody (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: The primary antibodies and concentrations used were: mouse anti-alpha tubulin (1:500; Sigma-Aldrich, Inc. Cat.# T9026. RRID:AB_477593). Secondary antibodies are listed in the Key Resources table.
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bioRxiv - Synthetic Biology 2019Quote: ... After rinsing 30 mL of 3% BSA TBST buffer with 0.0625 µL of anti-mouse antibody (Sigma-Aldrich, A9044) was added ...
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bioRxiv - Biochemistry 2020Quote: ... to detect the nanobodies and secondary antibody anti-mouse coupled to horse radish peroxidase (HRP) (0.5 μg/ml – Millipore). 50 μl of 1-Step UltraTMB-ELISA (ThermoScientific ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBST (1:2,000 for mouse anti-GFAP, Sigma; 1:1,000 for chicken anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated overnight at 4°C in mouse anti-NeuN primary antibody (#MAB377, RRID:AB_2298772, Millipore Sigma, Billerica, MA) at a 1:1000 concentration in diluted blocking solution (10% fetal plus ...
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bioRxiv - Neuroscience 2020Quote: ... or AAVretro-EF1a-Cre primary antiserum (1:100 mouse-anti-Cre Recombinase antibody, clone 2D8, Millipore Sigma Cat# MAB3120) mixed with donkey serum ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for immunoblotting at the indicated dilutions: mouse monoclonal anti-FLAG (Sigma-Aldrich, F1804), 1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed 3 times in PBS and incubated at room temperature for 30 min with a goat anti-mouse Ig polyclonal secondary antibody conjugated with alkaline phosphatase (0.5 µg/mL, Sigma). After 3 washes with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 3% milk in PBS + 0.05% Tween-80 (PBST) and then incubated overnight with primary antibodies (monoclonal mouse anti-FLAG [to detect MurJ; Sigma] ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 3-5 times with 1x TBST for a total of 20 min and then incubated for an hour with 1:3,000 dilution of secondary antibody (goat anti-mouse alkaline phosphatase conjugate, Sigma). The membrane was washed again with 1x TBST 3-5 times for a total of 20 min and developed using 5-bromo-4-chloro-3-indolylphosphate (BCIP)/ nitro-blue tetrazolium (NBT ...
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bioRxiv - Pathology 2022Quote: ... and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94,Sigma-Aldrich, St. Louis, MO) or 1:1000 anti- CD163 monoclonal antibody (Novus Biologicals ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were washed and then treated with a 1:1000 dilution of mouse anti-FLAG antibody (clone M2, Sigma) for 60 minutes then washed 3x in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Every 10 μg of chromatin was incubated with 2 µg of mouse M2 anti-FLAG monoclonal antibodies (Sigma-Aldrich) overnight at 4° C on a nutator ...
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bioRxiv - Physiology 2023Quote: ... and subsequently used for IP with 48 μg of an anti-NaVPAN mouse monoclonal antibody (mαNaVPAN, Sigma-Aldrich, #S8809), raised against the SP19 epitope (Vassilev et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were incubated with 15 μg of mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich, F1804, RRID: AB_262044) in 1 mL of PBS containing 0.5% BSA and 0.1% Triton X-100 at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the brain slices were washed 3 times using 1 x PBS in a shaker and incubated in blocking solution (10% normal goat serum and 0.2% Triton X-100 in PBS) for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... samples stained with primary FBXO11 (mouse) and LONP1 (rabbit) antibodies were incubated with Anti-Rabbit PLUS (Sigma-Aldrich DUO92002) and Anti-Mouse MINUS (Sigma-Aldrich DUO92004 ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody solution at 4 °C overnight (mouse anti- FLAG (1:1000; Sigma-Aldrich; F1804), mouse anti-RpS6 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed 3 times for 5 minutes with TBS-T and incubated in secondary antibody 1:10,000 dilution goat anti-mouse HRP (Sigma).
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bioRxiv - Immunology 2024Quote: ... nonspecific antibody binding was blocked by incubation with an anti-CD16/32 antibody (clone 2.4G2; UCSF Ab production facility) and whole mouse IgG (Sigma) on ice for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used for a western-blot experiment using a mouse-anti-Puromycin antibody (1:5,000, Millipore MABE343). To detect primary Ab ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore Sigma; MA, USA) or 1:200 goat anti-DCX (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: ... the tissues were incubated with primary mouse anti–α-actinin (sarcomeric) antibody (1:750, Sigma-Aldrich, cat. no. A7811), and vimentin (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated overnight at 4°C in blocking buffer with primary antibody (1:500 mouse anti-Elastin, Millipore Sigma MAB2503), washed ...
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bioRxiv - Microbiology 2023Quote: The following primary antibodies were used for immunoblotting: mouse monoclonal anti-α- tubulin (#T5168, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-MX1(MxA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...