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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... which uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate with ALP enzyme (Sigma Aldrich) as a standard[49].
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bioRxiv - Biochemistry 2022Quote: ... 0.1% TritonX-100) containing protease and protein phosphatase inhibitor cocktails (Sigma Aldrich, Gillingham, Dorset, UK). Protein concentrations were measured with BCA protein assay and samples were boiled for 5 min at 90°C after addition of 5%β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562, Sigma-Aldrich).
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 0.3% ß-mercaptoethanol) supplemented with 1.5% phosphatase inhibitor cocktail 2 (Sigma-Aldrich, St. Louis, MO). After adding extraction buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Hybridization signals were detected using alkaline phosphatase-conjugated anti-DIG antibody (1:1000, Merk Millipore), followed by incubation with 0.11 mM nitro blue tetrazolium and 0.12 mM 5-Bromo-4-chloro-3-indoyl phosphate in 6% polyvinyl alcohol ...
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bioRxiv - Physiology 2022Quote: ... and bromophenol blue) along with protease and phosphatase inhibitors (Sigma P8340 and Sigma P5726, respectively) were added to each of the samples ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with protease and phosphatase inhibitor cocktail (Cat. No. PPC1010, Sigma-Aldrich, St. Louis, MO) as described previously.(26 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mM EDTA) containing protease and phosphatase inhibitor cocktails and phenylmethyl sulfonyl fluoride (Sigma-Aldrich). BCA protein analysis (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mmol/L MgCl2] with antiproteases and anti-phosphatases (Sigma-Aldrich, St. Louis, MO, USA) in a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... PMSF 0.1 mg/mL) supplemented with proteinase and phosphatase inhibitors cocktail (1:100; Sigma Aldrich). Lysates were centrifuged at 13,000× g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Nonidet P-40 (v/v) with phosphatase inhibitors (Pierce, A32957 or Millipore-Sigma, 4906845001) and EDTA-free protease inhibitors (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Nonidet P-40 (v/v) with phosphatase inhibitors (Pierce, A32957 or Millipore-Sigma, 4906845001) and EDTA-free protease inhibitors (Pierce ...
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bioRxiv - Physiology 2024Quote: ... A dilution of 1:1,000 protease and phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) was added to the solution ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed in protein lysis buffer (Cell Signalling) supplemented with phosphatase (Phos-Stop, Sigma) and protease (Complete Mini ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with protease and phosphatase inhibitor cocktails (Cat. # PPC1010, Sigma-Aldrich, St. Louis, MO, USA). Protein samples were made and immunoblotting was performed as described previously4,6 ...
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bioRxiv - Developmental Biology 2023Quote: ... probes were detected using the alkaline phosphatase-coupled anti-DIG antibody (1:2000, Roche/Sigma) and NBT/BCIP (Roche/Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Appropriate secondary antibodies were used: phosphatase alkaline-conjugated goat anti-mouse or anti-rabbit (Sigma) along with the NBT and BCIP (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µM benzamidine) and phosphatase saturating substrate (para-nitrophenolphosphate [pNPP]; Sigma-Aldrich; 10 mM). Chromosomal DNA was mechanically sheared by passing through a metal needle ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 μg/ml phenylmethylsulfonyl fluoride and the cocktail of protease and phosphatase inhibitors (Merk/Sigma) (Russo et al. ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40) was supplemented with EDTA-free Halt protease and phosphatase inhibitor cocktail (Sigma) and applied directly to PBS-rinsed Toxoplasma-infected monolayers ...
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bioRxiv - Biochemistry 2023Quote: ... The binding of the alkaline phosphatase-conjugated antibodies was monitored with SigmaFAST BCIP/NBT (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... with protease inhibitor cocktail (1:1,000) and phosphatase inhibitor cocktail (1:400; Sigma-Aldrich/Merck) by sonication (VCX 130 PB ...
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bioRxiv - Cell Biology 2024Quote: Cultured cells were lysed in radioimmunoprecipitation assay buffer supplemented with protease and phosphatase inhibitors (Sigma). 10-30 μg of protein was resolved by electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested with ice-cold PBS supplemented with protease and phosphatase inhibitors (Sigma-Aldrich). Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested with ice-cold PBS supplemented with protease and phosphatase inhibitors (Sigma-Aldrich). Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... protease inhibitor and phosphatase inhibitor cocktails (1% v/v, P8340 and P5726, respectively, Sigma-Aldrich) and 1% SDS ...
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bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
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bioRxiv - Biochemistry 2022Quote: K562 cell line derivatives were collected by centrifugation at 1400 x g for 3 mins and nuclei were isolated using the Nuclei EZ Prep nuclei isolation kit (Sigma- Aldrich). The nuclei were fixed in 4% (v/v ...
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bioRxiv - Genetics 2022Quote: ... The external ammonium concentration was estimated as a function of time for 3 hours using the below-mentioned principle (Ammonia Assay Kit - Sigma-Aldrich). At each time point ...
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...
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bioRxiv - Immunology 2020Quote: ... The pro-inflammatory cytokines in the supernatant were analyzed by multiplex ELISA (Millipore, HCYTMAG-60K-PX29) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Nunc 96-well ELISA plates were coated with 50 µl of 2 µg/ml SA (Sigma) in borate saline buffer (100 mM boric acid ...
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bioRxiv - Neuroscience 2022Quote: ... sandwich ELISA of tau-5 coating antibody (20 µg/ml; Millipore Cat# 577801-100UG RRID: AB_212534) with either biotin-conjugated HT-7 (for human tau ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed in ELISA wash buffer (0.01M PBS with 0.05% Tween 20; Sigma; P7949) for 4x 2 minutes on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysate was added overnight to an ELISA plate precoated with anti-Itgb1 antibody (Sigma#MAB1997), the plates were then washed and the Itgb1 levels measured upon addition of HRP-streptavidin using a microplate reader at 405 nm with ABTS substrate ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... 3-dpf larvae were anesthetized with 0.016 % Tricaine/Ethyl 3-aminobenzoate methanesulfonate salt (A5040, SIGMA-ALDRICH) and microinjected with 5-8 nL of liposome encapsulated clodronate (SKU# CLD-8909 ...
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bioRxiv - Bioengineering 2021Quote: ... scaffolds were rehydrated in PBS overnight with 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDAC, Sigma-Aldrich) and N- hydroxysuccinimide (NHS ...