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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 10% biotin-labeled porcine tubulin and 4.5% rhodamine-labeled porcine tubulin was incubated with 2 mM GTP (Sigma-Aldrich) and 20 µm Taxol at 37°C for 35 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 8000 g for 10 min and pre-cleared by pre-incubated with 100 μl Mouse IgG−Agarose beads (A0919 Sigma) without antibody for 2 h at 4°C on wheel ...
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bioRxiv - Cell Biology 2020Quote: ... or 10μg of p53 antibody (Santa Cruz, DO1) (rabbit or mouse IgG was used as a negative control (Sigma, M7023)) for 4 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies were detected by incubation for 1 h with FITC-conjugated goat anti-mouse IgG (1:40, Sigma F8264) or Alexa Fluor 568-conjugated goat anti-mouse IgG (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed thrice with PBS-T followed by addition of peroxidase conjugated goat anti-mouse IgG diluted 15,000 fold and 20,000 fold (Sigma Aldrich, A4416) to RBD and N protein coated plates respectively ...
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bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Microbiology 2022Quote: ... cells were then incubated in a 1:5000 dilution of a horseradish peroxidase (HRP) conjugated goat anti-mouse IgG (Sigma). Titration of RUBV was performed similarly but with a polyclonal anti-RUBV goat IgG at 1:4000 (LifeSpan BioSciences ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used in this study were as follows: anti-mouse IgG whole molecule-Peroxidase (1:80,000; catalog no. A9044, Sigma-Aldrich) and anti-rabbit IgG whole molecule-Peroxidase (1:80,000 ...
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... Washing steps were performed using PBS-Tween buffer followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody (Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Biophysics 2023Quote: ... Anti-α-tubulin and anti-Nup153 antibodies were respectively revealed by ATTO647N goat anti-mouse IgG (1:400, Sigma-Aldrich) and STAR580 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... France) and secondary (anti-mouse IgG peroxidase-conjugated, 1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) antibodies were added at RT for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 3x with 200μLs of PBS-T per well and anti-mouse IgG HRP-conjugated secondary (Sigma, #AP308P) diluted at 1:3000 in blocking solution was added to each well and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then blocked with 5% milk or BSA in TBST and incubated with the indicated antibodies: mouse monoclonal anti-Tubulin (#T5168, Sigma, 1/10,000 dilution), rabbit anti-cleaved PARP (#9546 ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells producing HCMV specific antigens were detected 24h post infection by immunoperoxidase staining using monoclonal antibodies directed against the UL123-coded 72 kDa immediate early antigen 1 (IEA1) (Mouse Anti CMV IEA, MAB8131, Millipore, Temecula, CA, USA) and 120h post-infection by immunoperoxidase staining using monoclonal antibodies directed against UL55-encoded late antigen gB (LA ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Bioengineering 2021Quote: ... to visualize FA formation and α-smooth muscle actin (α-SMA, mouse monoclonal anti-α-SMA clone 1A4, Sigma-Aldrich, A2547, 1:400). Hydrogels were washed three times using PBS and incubated with secondary antibodies (AlexaFluor 488 goat anti-rabbit IgG or AlexaFluor 555 goat anti-mouse IgG ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-FLAG rabbit polyclonal and mouse monoclonal (Cat# F7425, RRID: AB_439687 and Cat# F1804, RRID: AB_262044, respectively, Sigma-Aldrich, St. Louis, MO, USA), anti-GFP rabbit polyclonal (Cat# A-11122 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Sections of the brainstem that contained CTB labeled neurons or mCherry neurons were incubated in a solution containing a mouse anti-TH monoclonal antibody (1:10,000; T-2928, Sigma-Aldrich, Oakville, ON, Canada) for 2 days ...
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bioRxiv - Genetics 2022Quote: ... For normalization of signals, we used actin or Pgk1, which was detected by using mouse anti-actin monoclonal antibody (1:5000, (Millipore Cat# MAB1501, RRID:AB_2223041)) or mouse anti-Pgk1 antibody (1:10000 ...
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bioRxiv - Cell Biology 2019Quote: Antibodies used in immunofluorescence and Western blot include: anti-MMP9 rabbit polyclonal antibody (# AB13458) and anti-GAPDH mouse monoclonal antibody (# CB1001) purchased from EMD Millipore (Billerica, MA); anti-Src rabbit antibody (# 2108) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... rabbit polyclonal anti-p[Thr202]ERK44/p[Thr204]ERK42 (1:10,000; product # 9101, Cell Signalling Technology) or mouse monoclonal anti-α-tubulin (1:50,000; product # T5168, Sigma-Aldrich, Oakville, Ontario, Canada). Membranes were then rinsed 4 times for 5 minutes with 0.1 % Tween 20/tris-buffered saline at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... membranes were incubated overnight on gentle agitation at 4°C with primary antibodies (TH, 1:1000; GAPDH, Sigma, G8795, mouse monoclonal, 1:10,000) diluted in antibody buffer (1:1 of Tris buffered saline (TBS ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...