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Citations for Millipore Sigma :
251 - 300 of 9777 citations for Recombinant Human LGALS14 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant protein was concentrated by centrifugation in Amicon® Ultra-15-30K (Millipore Sigma, Burlington, MA), purified on protein A/G columns (NAb™ Spin Kit ...
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bioRxiv - Biochemistry 2024Quote: Recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Biochemistry 2023Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant MejAgo protein expression and Ni-affinity chromatography purification procedures were performed according to user manual(Novagen). In perticular ...
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bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...