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Citations for Millipore Sigma :
251 - 300 of 4545 citations for Recombinant Human Hexosaminidase A alpha polypeptide His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Microbiology 2020Quote: ... alpha-tubulin was detected by anti-alpha-tubulin mouse monoclonal antibody (Sigma-Aldrich) and the corresponding goat anti-mouse IRDye 680 secondary antibody (Li-Cor Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic activity of beta-hexosaminidase (combined A and B isoforms) was determined by hydrolysis of 4-methylumbelliferyl-N-acetyl-β-glucosaminide (EMD Millipore Chemicals, Darmstadt, Germany) using 1.25 mM substrate in the incubation mixture ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Netherlands) and 48 hours later with 7.5 IU recombinant human chorionic gonadotrophin/hCG (Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 25 ng/ml of recombinant human Epidermal Growth Factor (EGF) (Sigma, Sitnyakovo Blvd, Bulgaria). Cells were then filtered through a 100 μm filter to retain the non-digested fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... treated twice every 12 h with human recombinant sDLL4 (1µg/ml, Cat#: SRP3026, Sigma Aldrich) for a total of 24h ...
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Genomics 2020Quote: ... and alpha-tubulin (Sigma Aldrich). Quantification of gel images was performed with ImageJ software and expressed as abundance in relative units to alpha-tubulin.
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bioRxiv - Cancer Biology 2020Quote: ... Alpha-catenin (C2081, SIGMA-ALDRICH), E-cadherin (3195 Cell Signaling) ...
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bioRxiv - Immunology 2021Quote: ... mouse monoclonal alpha-tubulin (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... alpha-tubulin (Sigma-Aldrich T5168), V5 (Invitrogen R960-25 ...
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bioRxiv - Microbiology 2020Quote: ... or alpha lytic protease (Sigma). Formic acid was then added to a final concentration of 2% and the samples were centrifuged at 14,000 rpm for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5mM alpha-ketoglutarate (Sigma), where indicated ...
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bioRxiv - Physiology 2019Quote: ... and alpha tubulin (Sigma T9026) were used in 1:2000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-alpha-tubulin antibody (Millipore) cat ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-alpha tubulin (T9026 Sigma), and appropriate fluorescently labelled secondary antibodies (Alexa-488 labelled anti-mouse (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 50μg/ml alpha-factor (Sigma) was added immediately to cultures and incubated at 30°C for 90 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... alpha-tubulin (B512, T6074, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... anti-tubulin alpha T5168 (Sigma).
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bioRxiv - Microbiology 2020Quote: ... alpha-lytic protease (Sigma-Aldrich) and Glu-C (Promega) ...
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... alpha modification media (Sigma Aldrich) supplemented with 1% L-glutamine (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-alpha-tubulin (Sigma #T5168); anti-LC3B (CST #83506) ...
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bioRxiv - Cell Biology 2023Quote: ... alpha-actinin (1:100, Sigma), and cardiac troponin I (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... alpha-tubulin (Sigma, Cat# T5168), MLL1 (1:1,000 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and alpha-cypermethrin (Sigma-Aldrich, product # ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
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bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2020Quote: ... Alpha tubulin was detected by the monoclonal mouse anti-alpha tubulin antibody (1:20’000, Sigma) and the rabbit anti-mouse-IgG conjugated to horseradish peroxidase (1:10’000 ...
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bioRxiv - Developmental Biology 2020Quote: ... alpha-tubulin and histone H3 were detected using monoclonal antibody (mouse anti-alpha-Tubulin, Sigma T9026 or rat anti-Tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...