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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... In the reciprocal experiment 1 mg of N-terminally his-tagged Saci_1104 was loaded onto a Nickel-NTA (Sigma, Germany) column (column volume of 250 µl) ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Immunology 2022Quote: ... human RBCs were biotinylated with controled concentration of biotin-X-NHS linker (Millipore/Sigma). The biotinylated RBCs were sequentially incubated with saturating amount of streptavidin (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... human RBCs were biotinylated with controled concentration of biotin-X-NHS linker (Millipore/Sigma). The biotinylated RBCs were sequentially incubated with saturating amount of streptavidin (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Physiology 2020Quote: ... then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma Aldrich) at ZT6 (time “0 minutes”).
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bioRxiv - Biochemistry 2021Quote: Human recombinant SRPK2 (0.4 ug/reaction; EMD Millipore 14-666; Glu46-end) and SRSF2 (0.8 ug/reaction ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 400ng/mL of human recombinant leptin (Sigma #L4146). In leptin neutralization studies ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were directly labeled using alkaline phosphatase-coupled streptavidin (Sigma-Aldrich). The blots were developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... The recombinant protein was produced in Rosetta (DE3) cells (Novagen) and purified by affinity chromatography using a GSTrapFF column ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were created using the pET expression system (Novagen). The codon-optimized typical faeG gene of the original K88 (K88ac variant ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... active recombinant proteins were purchased from Sigma (SRP0364, P0066, respectively). Recombinant purified human MASTL protein was used as a substrate and was from Abnova (H00084930) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were expressed in either BL21 or Rosetta (Novagen) cells ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... LPA and recombinant Src protein were purchased from Sigma-Aldrich, while recombinant human IDO1 protein was obtained by Giotto Biotech ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant protein production was induced via IPTG induction (Sigma). Cells were lysed with BugBuster HT (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...