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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Human CSF2 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Immunology 2019Quote: ... Streptomyces hyaluronlyticus hyaluronidase (HA’se) and biotinylated HA-binding protein (HABP) were from Millipore, and streptavidin was from Thermo Fisher Scientific or eBioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Immunology 2021Quote: ... and maternal plasma were determined by Luminex using non-human primate multiplex kits (Millipore). Lipids were extracted from the AF using methanol to measure prostaglandins PGE2 (Oxford Biomedical Research ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged proteins were immunoprecipitated using 20 μl M2 beads (Sigma Cat. #A2220) and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... Flag-tagged protein was purified using 20µl anti-Flag® M2 magnetic beads (Sigma) per plate ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Biophysics 2022Quote: ... The non-reacted biotin was then removed by passing the biotinylated sample through a PD SpinTrap G-25 column (Sigma-Aldrich) with a molecular weight cutoff of 5000 Da ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Immunology 2021Quote: ... Non-specific protein was blocked with 2 % normal donkey serum (Sigma Aldrich) in PBS containing 0.05 % Tween-20 and 1 % BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore, Darmstadt ...
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bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
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bioRxiv - Cell Biology 2019Quote: Morphogenesis was induced by the addition of recombinant human HGF (product H1404, Sigma-Aldrich) and conditioned media from NIH/3T3 cells (product CRL-1658 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Molecular Biology 2024Quote: ... biotinylated proteins bound to streptavidin beads were resuspended in 100 mM ammonium bicarbonate (Sigma) and digested with 2 µg Trypsin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Genetics 2023Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Microbiology 2024Quote: ... Non-specific binding sites on the bacterial cells were blocked using human IgG (Sigma, UK) for an hour and washed with flow buffer again before incubated overnight with the rabbit immune sera (1:1,000 dilution) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...