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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... or IgA (α-chain specific, A9669, Millipore-Sigma) conjugated to alkaline phosphatase was used as secondary antibody ...
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bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
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bioRxiv - Cancer Biology 2020Quote: ... In the RAS activation assay kit for ELISA (Cat.#17-497; EMD Millipore), 12 µg total protein was used at 100 ng/µL and the RAS-GTP pull-down was measured using a Synergy H1 plate reader (BioTek ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured with Human Insulin ELISA kit (Millipore Sigma). See Table S1 for donor information.
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bioRxiv - Biochemistry 2019Quote: ... Plasma leptin levels were analysed with an ELISA kit (Millipore, Billerica, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Level of serum insulin was determined using insulin ELISA kit (Millipore-EZMRI-13K).
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bioRxiv - Cell Biology 2023Quote: ... These evaluations were performed by the commercial Human ELISA kits (Sigma-Aldrich, USA) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... GLP-1 levels were measured using a total GLP-1 ELISA kit (Millipore) and an Infinite 200 Pro Reader (TECAN ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma leptin and insulin levels were measured by murine ELISA kits (Merck Millipore, Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... which was analyzed by enzyme linked immunosorbent assay (ELISA) kit (Sigma-Aldrich, MO). The levels of Citrine-H3 (Cit-H3 ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Neuroscience 2019Quote: ... To control for loading differences we probed with anti-gamma tubulin (Sigma, T5326) or GAPDH (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were precipitated twice by addition of 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Neuroscience 2020Quote: ... Lipopolysaccharide (LPS, serotype O55:B5, gamma-irradiated) was purchased from Sigma (Sigma L6529), recombinant mouse IL-1β was purchased from Cedarlane (Cedarlane CLCYT273) ...
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bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
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bioRxiv - Immunology 2023Quote: ... Phosphorylated ERK was measured by preparing epithelial cells as described above (Colon epithelial cell isolation) and using a Phospho-Erk1 (pThr202 / pTyr204 + Erk2 (pTyr185/187) and pan-Erk1 / 2 ELISA Kit from Sigma (Cat. RAB0349) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Fused cells (myotubes) were visualized using an antibody against Myosin heavy chain isoforms (MyHC) (MF20, Sigma-Aldrich). Primary antibodies were visualized with anti-rabbit or anti-mouse conjugated with Alexa 488 or Cy5 (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated on rat tail collagen (Sigma-Aldrich, 11179179001) coated glass slides ...
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bioRxiv - Cell Biology 2019Quote: ... A7r5 rat smooth muscle embryonic aorta cells (Sigma Aldrich, #86050803) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: Rat insulinoma cell line INS-1 832/3 (Millipore Sigma) was cultured in RPMI-1640 supplemented with 2mM L-Glutamine ...
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bioRxiv - Genetics 2023Quote: ... cells were incubated with FITC anti-rat IgG (Sigma-Aldrich) and AF633 anti-rabbit IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were stimulated with PMA (20 ng/ml; Sigma-Aldrich) and ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... helper T cells were restimulated with 200 nM PMA (Sigma-Aldrich) plus 1 μg/ml of ionomycin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were maintained in RPMI-1640 medium (Sigma Aldrich) supplemented with 10% FBS and 100 μg/ml penicillin-streptomycin at 37 °C in a humidified ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were stimulated with PMA (20 ng/ml; Sigma-Aldrich) and ionomycin (1 μg/ml ...
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bioRxiv - Physiology 2023Quote: CD4+ T cells were lysed in RIPA buffer (Sigma-Aldrich, R0278) containing protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2023Quote: γδ T cells were stimulated with 100 ng/mL PMA (Sigma-Aldrich) and 1 μg/mL ionomycin (from Streptomyces conglobatus ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete T cell medium consists of RPMI 1640 (Sigma-Aldrich, R8758) with 10% FBS and supplemented with 1:100 Minimum Essential Medium Non-Essential Amino Acids (MEM NEAA ...
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bioRxiv - Microbiology 2024Quote: ... Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich) supplemented with 5 mM HEPES (Corning) ...
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T cells pre-stimulated with PHA (Sigma, 1µg/ml) and 10 ng/ml IL-2 (Stemcelll ...
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bioRxiv - Immunology 2024Quote: ... T cells were incubated with 10 μM Sal003 (Sigma-Aldrich, S4451), 20 μM Sephin1 (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: Anti-CD3 antibody was from Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2022Quote: ... Secretion of the following cytokines was quantified with the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex - Immunology Multiplex Assay kit (EMD-Millipore): IL-6 ...
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bioRxiv - Immunology 2019Quote: Lactate concentration in the extracellular medium of T cells cultured under Th17-polarizing conditions for 96h was performed using the Lactate Assay kit from Sigma-Aldrich according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
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bioRxiv - Physiology 2022Quote: ... or ELISA (ghrelin; Millipore).
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bioRxiv - Neuroscience 2021Quote: ... GFAP was also measured in the supernatant with a sandwich ELISA kits (Sigma NS830) using a 200x dilution ...
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bioRxiv - Physiology 2019Quote: ... P10 and 10 weeks of age using a commercially available leptin ELISA kit (Millipore). Serum free fatty acid levels were assayed in chow-fed or HFHS-fed mothers at gestational day 16 and in the offspring of chow- or HFHS-fed dams at P10 and 10 weeks of age using a commercially available FFA kit (Abcam).
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bioRxiv - Microbiology 2023Quote: ... and IL-18 (BMS672TEN) in supernatants were measured using the ELISA Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neuropeptide Y levels were measured using commercially available ELISA kits (EMD Millipore, EZHNPY-25K).
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bioRxiv - Cell Biology 2019Quote: ... cells were transferred to ice and surface biotin was cleaved with 60 mM MesNa (Sigma, 63705) in MesNa buffer (50 mM Tris-HCl [pH 8.6] ...
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bioRxiv - Immunology 2022Quote: ... Cells were processed for surface staining as described above and fixed with 2% paraformaldehyde (Sigma-Aldrich) followed by permeabilization using 1X Permeabilization buffer (BDbiosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-polyubiquitin K63-linked chains was from Millipore (#051308). The secondary antibodies anti-rabbit IgG-HRP (#111-035-144 ...
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bioRxiv - Neuroscience 2021Quote: ... 1:1000 Myosin Heavy Chain 1 (05-716, Millipore), 1:5000 mCherry (ab205402 ...
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bioRxiv - Neuroscience 2023Quote: ... and NFH (neurofilament heavy chain, Cat#AB5539, Merck Millipore). We used Alexa Fluor 555nm labelled goat anti-mouse secondary antibody (Cat#A28180 ...
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bioRxiv - Biophysics 2023Quote: The rat cardiomyoblast cell-line H9c2 (cells derived from embryonic heart tissue; Sigma Aldrich) was genetically modified using retrovirus to achieve a stable expression of tandem tagged (mCherry-EGFP ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...