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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Myelin protein P0 MPZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma). OPCs were purified by immnopanning approach according to our previous protocol (Lang et al. ...
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bioRxiv - Genetics 2021Quote: ... Neural progenitor cells were frozen at stage zero (P0) and subsequently thawed in ENSTem-A Neural Expansion Medium (Millipore Sigma) for differentiation ...
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bioRxiv - Neuroscience 2019Quote: The anterior half of cortices from P0 mice were dissociated then cross-linked with 1% formaldehyde and processed using EZ-ChIP kit (Millipore). Chromatin fragments bound by endogenous mouse SOX5 were pulled down by anti-SOX5 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Hippocampi were isolated from mouse embryos (E18) or newborn pups (P0) and digested with 0.25% trypsin-EDTA (1x) (Sigma, T4549) at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... P0-P1 cortices were lysed at 50 mg/mL in AP buffer supplemented with protease and phosphatase inhibitors (Sigma-Aldrich), then subjected to vortex/ice cycles for 20 min ...
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bioRxiv - Neuroscience 2024Quote: Primary rat hippocampal neurons were digested from dissected rat brains at postnatal day 0 (P0) and seeded on 14-mm glass coverslips pre-coated with 20 μg/mL poly-D-lysine (Sigma) and 10 μg/mL laminin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2022Quote: ... and anti-His (1:3000) antibody (Sigma). HRP conjugated mouse IgG was used at 1:5000 dilution (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His HRP conjugated (Sigma-Aldrich) antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIS-Select® Nickel Affinity resin (Sigma) and Streptactin column material (IBA GmbH ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... anti-His monoclonal antibody (Sigma, 1:1000) and anti-mouse (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with His-HDAC6 (EMD Millipore) and 1 mM ATP in kinase buffer for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... then detected with anti-His antibody (Sigma). S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex) ...
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bioRxiv - Immunology 2020Quote: ... with added 10% HI FBS (Sigma-Aldrich) and 1% Pen/Strep (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-HIS (1:10,000, Sigma H1029) was used ...
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Molecular Biology 2019Quote: ... Black Gold staining was conducted according to the manufacturer’s instructions (Black Gold II myelin staining kit, EMD Millipore, Billerica, MA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was discarded and myelin was removed by centrifugation at 2600 r/min for 10 min in 22% bovine serum albumin (BSA, Sigma). The microvascular segments and cells were resuspended by using the Endothelial Cell Growth Media (ECGM ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi from postnatal mice (p0-p3) were surgically isolated and dissociated into single cells by trypsin (5 g/L, Sigma-Aldrich) digestion ...
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bioRxiv - Cell Biology 2023Quote: ... pregnant dams at E15.5 gestation or newborn pups at P0 were injected intraperitoneally with a single 200 mg/kg dose of a solution containing tamoxifen (Sigma-Aldrich, T56480 ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary cortical neurons derived from Lrrk2 WT C57BL/6 and KO pups (P0) were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS1X pH 7.4 for 20 minutes at room temperature and ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...