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Citations for Millipore Sigma :
251 - 300 of 4781 citations for IL 7 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 7-Deacetyl-7-[O-(N-methylpiperazino)-gamma-butyryl]-dihydrochloride (20µM; 344273, EMD Millipore, Billerica, MA) was dissolved in sterile Milli-Q water ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... HEK293 cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Immunology 2021Quote: ... 10ng/ml IL-4 (Sigma), 40nM LY2090314 (Sigma).
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bioRxiv - Immunology 2020Quote: ... IL-2 (I2644, Sigma-Aldrich); αCD3 (40-0038 ...
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bioRxiv - Molecular Biology 2021Quote: ... and IL-6 (Sigma-Aldrich) treatments were performed at a concentration of 50 units/mL and 50 ng/mL in cDMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... The exclusion of dead cells was done by adding 7-aminoactinomycin D (7-AAD) (Sigma-Aldrich) to the cell suspension to the final concentration of 1 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... 7 ng/mL glucagon (Sigma-Aldrich), 0.1 mM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 7 μM Hemin chloride (Sigma-Aldrich) was added to the culture medium and kept in the medium until the fixation step (below) ...
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bioRxiv - Cell Biology 2019Quote: ... 7 µl 2-mercaptoethanol (07604, Sigma) and 690 µl Buffer B (50 mM Tris ...
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bioRxiv - Genetics 2019Quote: ... 7 mM caffeine (C0750, Sigma-Aldrich), 100 mM hydroxyurea (H8627 ...
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bioRxiv - Neuroscience 2019Quote: ... GLUR6/7 (04-921, EMD Millipore), EEA1 (sc-6415 ...
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bioRxiv - Genomics 2019Quote: ... and 1.0M pH 7 from Sigma. Triethylamine acetate buffer 2.0M pH 7 and Sodium hydroxide 1.0M bioreagent were purchased from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... and 10−7 M insulin (Sigma). The number of viable cells was determined using trypan blue (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... NBQX (2 μg, Sigma, n=7), Bicuculline methiodide (0.05 μg ...
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bioRxiv - Biochemistry 2021Quote: ... mAb-HA (Clone 7, H9658, Sigma), pAb-HA (Signalway Eurogentec) ...
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bioRxiv - Biophysics 2020Quote: ... and ML-7 (I2764, Sigma-Aldrich) were used at 5 μM working concentration ...
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bioRxiv - Bioengineering 2020Quote: ... Glucose (50-99-7, Sigma-Aldrich), DMEM High glucose (4.5g/L ...
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bioRxiv - Plant Biology 2021Quote: 2’,7’ dichlorofluorescein diacetate (H2DCFDA, Sigma) was used to measure ROS in guard cells (49) ...
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bioRxiv - Biochemistry 2021Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and 7 M urea (Sigma Aldrich)) in 1 x TBE (Tris-borate EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... 7 μg/ml gentamycin (#G1372, Sigma), 10 μg/ml tetracycline (#T3258 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA (HA-7, Sigma-Aldrich), anti-KLC1 ([EPR12441(B)] ...
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bioRxiv - Cancer Biology 2021Quote: ... Hyaluronidase (Sigma, #H3884, 7 mg/ml) and DNase I (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7 (TEAB, Sigma-Aldrich, 18597) and loaded on an S-trap filter (Protifi ...
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bioRxiv - Microbiology 2021Quote: ... 7 ng/mL glucagon (Sigma-Aldrich), 40 ng/mL dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... benzaldehyde (Sigma CAS #100-52-7), ethyl butyrate (Sigma CAS #105-54-4) ...
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bioRxiv - Microbiology 2021Quote: ... 7 μM CuCl2 (Sigma-Aldrich 307483), 7.1 μM MnSO4 (Sigma-Aldrich M8179) ...
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bioRxiv - Bioengineering 2022Quote: ... 10−7 M dexamethasone (D4902, Sigma) and 10 ng/mL TGF-β3 (Novartis) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 7%KCl (Sigma Aldrich, Germany). We also grew the cells in 3X TSB containing 0.3M sucrose ...
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bioRxiv - Neuroscience 2023Quote: ... 7-benzyloxy-4-trifluoromethylcoumarin (BFC, Sigma), for 90 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-7 M dexamethasone (D2915, Sigma), and 10 ng/mL TGF-β3 (100-36E ...
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bioRxiv - Biochemistry 2023Quote: ... containing 7 M urea (Sigma Aldrich) in 1× TBE (Tris-borate EDTA ...
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bioRxiv - Plant Biology 2023Quote: ... Cellobiose was obtained from Sigma (Sigma-Aldrich; Cas: 528-50-7). To investigate the activity of Cel7A on acetobacter cellulose as a function of cellobiose concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-7 M dexamethasone (D2915, Sigma,), and 10 ng/mL TGF-β3 (100-36E ...
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bioRxiv - Cell Biology 2023Quote: ... 10−7 M dexamethasone (Dex) (Sigma), 10 mM nicotinamide (Sigma) ...