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Citations for Millipore Sigma :
251 - 300 of 2052 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Herring-testis DNA was obtained from Sigma. cGAMP and poly I:C were from Invivogen ...
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bioRxiv - Immunology 2023Quote: ... DNA was visualized using DAPI (Sigma-Aldrich). Quantification of fluorescence will be performed at ×20 magnification in one random microscopic field of each kidney ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with Hoechst 33342 (Sigma). Images were analyzed using either Leica DMI4000 B fluorescent microscope or Leica TCS SP5 confocal microscope.
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bioRxiv - Biochemistry 2023Quote: ... DNA oligomers were obtained from Sigma-Aldrich unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was stained with HOECHST 33258 (Sigma). Immunostainings for the 1st and 2nd antibodies were performed in 5% NHS in PTX ...
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bioRxiv - Genetics 2024Quote: The DNA polymerase inhibitor aphidicolin (APH, Sigma) was dissolved in DMSO at a stock concentration of 200µM ...
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bioRxiv - Cell Biology 2024Quote: ... coli DNA (Sigma D-2001, type VIII), sheared as described (56) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Immunology 2019Quote: ... TBK1 (SHCLND-NM_013254) or non-Target shRNA Control Plasmid DNA were all obtained from Sigma (MISSION shRNA Plasmid DNA). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... samples containing 10-30 µg genomic DNA were treated with Benzonase nuclease (EMD Millipore; 0.5 units per µg DNA) in the presence of 2 mM magnesium chloride for 30 min at 37°C to digest DNA ...
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bioRxiv - Plant Biology 2021Quote: Genomic DNA was extracted from leaves using the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, USA). The integrity of the DNA was verified by electrophoresis on a 1% agarose gel stained with ethidium bromide ...
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bioRxiv - Microbiology 2020Quote: Bacterial DNA was isolated and purified with the GenEluteTM Bacterial Genomic DNA kit (Sigma-Aldrich, St. Louis, Missouri, USA). PCR was used for the identification of six virulence factor genes (fliL ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA-calcium phosphate transfection complex was prepared by gently adding plasmid DNA and 2M CaCl2 (Sigma-Aldrich; Merck KGaA) dropwise to HBS buffer (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Sonicated and biotinylated DNA was prepared as follows: 250 μl of 10 mg/ml sonicated salmon sperm DNA (Sigma) was sonicated for an additional 5 min (Misonix sonicator 3000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was purified from cells harvested at the indicated time points using a Mammalian Genomic DNA Miniprep Kit (Sigma). DNA was immobilized on nitrocellulose and analyzed by immunodot blotting with antibodies against cyclobutane pyrimidine dimers (CPDs ...
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bioRxiv - Immunology 2022Quote: ... Genomic DNA was extracted from PBMCs using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, USA).
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from sub-cultured isolates (loop of colonies) using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The DNA sequence was amplified from RH genomic DNA and cloned into the pET-32 LIC/EK vector (Novagen), which adds an N-terminal thioredoxin and histidine tag to the expressed protein ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μg of plasmid DNA was transfected per plate by incubating the DNA with 10 μg polyethyleneimine (Sigma, 408727) in serum free media for 10 min before being added dropwise to cells that had been changed to serum free media 1 hour prior.
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2019Quote: ... TBK1 (SHCLND-NM_013254) or non-Target shRNA Control Plasmid DNA were all obtained from Sigma (MISSION shRNA Plasmid DNA). In brief ...
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bioRxiv - Genetics 2023Quote: ... Extracted DNA samples were quantified using lambda (λ) DNA molecular weight standards (Sigma-Aldrich Inc., St. Louis, Missouri, USA) of known concentrations in a 1% UltraPure™ agarose gel (Invitrogen Corp. ...
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bioRxiv - Cell Biology 2023Quote: ... DNA synthesis was quantified by the DNA incorporation of the thymidine analog 5-ethynyl-2′-deoxyuridine (EdU; Sigma Aldrich) (10 μM ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1 x 106 stable cells using the GenElute mammalian genomic DNA kit (G1N350, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... After 9 days 3-5 × 106 cells from selection conditions and 100 × 106 non-selection conditions were washed 3 times with PBS and pelleted by centrifugation for genomic DNA extraction using GeneElute Mammalian Genomic DNA Miniprep kit (Sigma) and Quick-DNA Midiprep Plus kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... 18 μL (1:3 ratio of DNA to transfection reagent) of X-tremeGENE™ 9 DNA transfection reagent (MilliPore Sigma). The mixture was incubated for 30 mins at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Repair templates and DNA fragments for cloning were generated by PCR amplification with either High Fidelity Hot Start KOD DNA Polymerase (Novagen) or Phusion Hot Start DNA Polymerase (Finnzymes) ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cisplatin-induced DNA damage was assayed using an antibody against the DNA double strand break marker gamma-H2AX (Merck-Millipore) on tumor sections co-stained with DAPI to enumerate cell nuclei ...
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bioRxiv - Genetics 2022Quote: ... To assess editing efficiency genomic DNA was isolated from CRISPR-edited and control cells using a GenElute Mammalian DNA Miniprep Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the PCR reaction described above was repeated using genomic DNA isolated using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... total DNA content was isolated from the 3D constructs at 14 and 21 days using a DNA extraction kit (Sigma). The isolated DNA contents were collected and quantified by using Nano drop (Thermo Scientific-2000).
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bioRxiv - Neuroscience 2022Quote: ... PEG 3350 40% v/v) with the DNA mix to be recombined and 50 μg of salmon sperm denaturated DNA (31149, Sigma) and then cooled for at least 5 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from fresh bacterial cultures of the strains of interest using the GenElute Bacterial Genomic DNA Kit (SIGMA) according to manufacturers instructions ...
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bioRxiv - Biochemistry 2021Quote: ... bacteriovorus HD100 genomic DNA using primers detailed in Table S1 Amplified construct DNA was inserted into a modified pET41c plasmid (Novagen, GST-tag removed and thrombin cleavable 8xHis-tag introduced at C-terminus ...
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bioRxiv - Neuroscience 2020Quote: APOE genotype was determined by extracting genomic DNA from participants’ blood samples using a GenElute Blood Genomic DNA Kit (Sigma). After confirming concentration and quality by Nanodrop ...
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bioRxiv - Genomics 2020Quote: ... Cells were harvested at μmax and genomic DNA was isolated using GenEluteTM Bacterial Genomic DNA Kit (NA2120-1KT, Sigma-Aldrich) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split 48 hours after transfection and DNA was extracted using Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich #G1N350). Non-homologous repair efficiency was evaluated by Sanger Sequencing using KAPA2G Taq polymerase (Kapa Biosystems #KK5601 ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from single colonies after culturing in TYG using the GenElute™ Bacterial Genomic DNA kit (Sigma) and screened for tag insertions by PCR using primers flanking various att sites ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were quantified by isolating the genomic DNA from 1ml of inoculate using GenElute Bacterial Genomic DNA kit (Sigma) and measuring the genomic equivalents (GE ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... DNA encoding the amino acids 264-1309 of dmIR was subcloned into the vector pBAC™4x-1 DNA (Novagen) downstream of the p10 promoter with the addition of a starting Methionine and C-terminal StrepII-tag ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...