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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Ceramide Synthase 3 CERS3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... An antibody specific for GroEL (rabbit polyclonal, 1:10,000 in 3% BSA/TBS-T; Sigma-Aldrich, #G6532-5ML, RRID:AB_259939) with an anti-rabbit IgG (goat polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... (TDP43: 5’-gcaaagccaagaugagccu-3’ and EGFP control: 5’-gcaccaucuucuucaagga-3’; Sigma). Silenced cells were collected by trypsinization ...
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bioRxiv - Neuroscience 2022Quote: ... prior to being rinsed and reacted using 3-3’diaminobenzidine (Sigma) as chromagen ...
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bioRxiv - Biochemistry 2021Quote: ... and 3’,3’c-di-AMP were purchased from Sigma-Aldrich, 2’,3’-cGAMP ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimidehydrochloride (EDC) were procured from Sigma Aldrich, USA.
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bioRxiv - Physiology 2019Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma-Aldrich, No. E7750) and an amide ...
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bioRxiv - Immunology 2019Quote: ... 3 ml of 3% bacteria-free thioglycollate solution (Sigma, B2551-500G) was injected into the abdominal cavity of C57/BL6 mice ...
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bioRxiv - Plant Biology 2022Quote: ... and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma). Biotin-labelled probes were hybridized with sRNAs on the nylon membrane and stabilized streptavidin-horseradish peroxidase was used to detect the biotin signal.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (w/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed cells were stained with the following antibodies (for 3 hrs at 37°C): β-Tubulin (1:100, Sigma-Aldrich), DBC1 (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... washed 3 times in PBS and then incubated for 30 min with secondary antibodies and DAPI (Sigma, 1:1000 dilution); all antibodies were diluted in PBS + 3% BSA and are as follows ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Plant Biology 2021Quote: ... PVDF membranes were blocked with 5% milk followed by an incubation with first antibodies for REM1.2/1.3 followed by 3 washes and an incubation with second α-rabbit antibody fused to HRP against rabbit (Sigma). Clarity Western 701 ECL (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... the labeled SPs or anti-ACE2 antibody were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate.
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bioRxiv - Neuroscience 2019Quote: ... Sheared crosslinked chromatin was immunoprecipitated separately with an anti-AR antibody (3 μg, 17-10489 ChIPAb + androgen receptor Assay Kit, Millipore) or an equal amount of normal IgG (3 μg ...
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bioRxiv - Developmental Biology 2019Quote: ... the supernatant was transferred into a new tube (and the DNA concentration determined by NanoDrop 2000 Spectrophotometer .25 μg of chromatin were used to immunoprecipitate with 3 μg of the rabbit anti H3K27me3 antibody (Millipore) previously bound to dynabeads overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 3% Triton-X in PBS) for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK ...
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blocking solution was discarded and replaced with 30 mL of 3% BSA TBST buffer then 7.8 µL of 3.85 mg/mL anti-FLAG antibody (Sigma-Aldrich, F3165) was added ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 1 hour in 3% BSA-TBS and incubated overnight at 4°C with the following primary antibodies: anti-phosphotyrosine 4G10 (Millipore), anti-phosphorylated SYK (pY525/526 ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed 3 x 5 min with 3% BSA in PBS-T and incubated with the following DNA dye and secondary antibody dilutions in blocking solution for 3 hours at room temperature: Hoechst 33342 (0.2 mM, Sigma-Aldrich), Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-HA gels were washed 3 times with 20 ml TBS-T and then incubated with peroxidase-conjugated secondary antibody (1:10,000; Sigma A0545) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... All antibody incubations were done in block solution containing 5-10% Normal Donkey serum and 3% Bovine Serum Albumin (BSA, Sigma). After early initial staining ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated for 45 min with the corresponding secondary antibody (Table 3) diluted in blocking buffer plus DAPI (Sigma) in darkness ...
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bioRxiv - Immunology 2024Quote: ... before microtubule-associated protein light chain 3 (LC3)-I and LC3-II levels in cell lysates was analysed by immunoblotting (rabbit polyclonal LC3 antibody, Sigma).
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Physiology 2024Quote: ... cells were incubated with blocking buffer (PBS containing 3 % milk and 0.1 % Triton X-100) and primary antibodies against ACTN2 (1:800, Sigma, A7811) and dTyr-tub (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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bioRxiv - Cancer Biology 2019Quote: ... ADORA1 selective and competitive antagonists 1-Butyl-3-(3-hydroxypropyl)-8-(3-noradamantyl) xanthine (PSB36) and 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) (Sigma-Aldrich); ADORA2b selective antagonist 4-(2,3,6,7-Tetrahydro-2 ...
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bioRxiv - Plant Biology 2020Quote: ... inflorescences were soaked in 3% (w/v) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Sigma Chemical, St. Louis, MO, USA, E6383) with 0.05% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...