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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... encoding a gRNA for human AAVS1 targeting (GGAAGAGAGTAGGTCGAAG) using GeneJuice Transfection Reagent (Sigma-Aldrich). Transfected cells were selected with either 0.5μg/mL puromycin or 100μg/mL neomycin ...
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bioRxiv - Bioengineering 2023Quote: ... human skeletal muscle biopsies were minced and incubated with collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence; EMD Millipore; catalogue number MABT201 ...
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bioRxiv - Cancer Biology 2023Quote: ... human PBMCs were labelled with the fluorescent membrane marker PKH26 (Sigma-Aldrich, MINI26-1KT). For the in vivo killing assay ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... Coverslips were blocked and permeabilized in staining buffer [1% human serum (Sigma Aldrich, 4552) heat-inactivated at 56 °C for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific L3000015 ...
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bioRxiv - Microbiology 2023Quote: ... Purified human IgG and IgA were used as standards (Sigma, St. Louis, MO, USA). Tear samples were tested by 4-fold serial dilution ...
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bioRxiv - Cell Biology 2023Quote: ... we used MISSION esiRNA for MDM4 (ESIRNA HUMAN MDMX, Cat. # EHU005381-20UG; Sigma-Aldrich) and MISSION esiRNA for Renilla Luciferase as a control (ESIRNA RLUC Cat ...
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bioRxiv - Biochemistry 2023Quote: Purified human NUDT15 (20 mg/mL) was preincubated with 10 mM GPP (Sigma-Aldrich) for 2 hours at 20 °C ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... females were injected with 5 I.U of human chorionic gonadotropin (hCG) (Sigma-Aldrich #CG5) to induce ovulation ...
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bioRxiv - Bioengineering 2024Quote: ... Human hepatic stellate cell line LX-2 were acquired from Sigma (cat. no. SCC064), and were cultured in DMEM-2 (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... Spermatozoa were capacitated in Human Tubal Fluid medium (HTF) (Merck Millipore; MR-070-D) supplemented with 10 mg/ml Albumin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... The 22Rv1 human prostate cancer cell line was grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then quickly coated with 100 ug/mL human fibronectin (Sigma #SLCL0793) diluted in PBS and injected using a pipette tip ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Patu8988S and MiaPaCa-2 human pancreatic cancer cells were cultured in RPMI (Sigma, #R1383) or DMEM (Gibco ...
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bioRxiv - Immunology 2024Quote: ... Human CD34+ cells were isolated from FL by density gradient centrifugation (Histopaque-1077, Sigma) followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Human cell lines: T47D (HTB-133, ATCC) were cultured in RPMI-1640 (R8758, Sigma) and supplemented with 0.2 unit/ml bovine insulin (I0516-5ML ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Immunology 2024Quote: The MILLIPLEX MAP Human CD8⁺ T-Cell Magnetic Bead Panel (Merck Millipore Darmstadt, Germany) was used to quantify GM-CSF ...
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bioRxiv - Cell Biology 2024Quote: ... NSCs were validated for expression of mouse anti-human Nestin (1:2,000; Millipore; MAB5326), rabbit-anti Sox2 (1:400 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG; Sigma-Aldrich). Alternatively ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Biophysics 2021Quote: ... FnCas9 protein was purchased from Millipore Sigma (FNCAS9PROT-250UG).
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were wet transferred onto PVDF membrane (Millipore, IPFL00010), blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... beads containing bound proteins were incubated with thrombin (Novagen) overnight at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore), and membranes were incubated in Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration in S3 was normalized via Bradford (SIGMA) to ensure that equal amount of protein was loaded before ultracentrifugation step ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein sample was transferred to PVDF membranes (Millipore) using Mini Trans-Blot (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein estimation was performed using Bradford assay reagent (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...