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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Molecular Biology 2019Quote: ... For Flag-tag immunoprecipitation samples were incubated with 100 µl of anti-FLAG M2 magnetic beads (#M8823, Sigma-Aldrich Chemie; Munich, Germany) for 4 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Immunology 2023Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30 µM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60 µM ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
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bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285) and intact cell respiration was normalized to milligrams of total intact cell protein.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
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bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich), 1% NEAAs and 1% penicillin-streptomycin and maintained at 39 °C in a 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2019Quote: ... purified DN3a cells were cultured on OP9-DL1 stromal cells or Fc-DL1 functionalised surfaces in the presence of AMD3100 (2 μg/ml, Sigma-Aldrich) or DBZ (0.05 µM ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: 4% PFA-fixed brains were washed twice in 20 mL PBS for a total of 24 hours and imaged in Fluorinert FC-40 (Sigma Aldrich) for null background signal ...
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bioRxiv - Bioengineering 2021Quote: Each intact piglet brain hemisphere was placed in a 30 mm diameter glass tube containing perfluorocarbon oil (Fluorinert FC-40, Sigma-Aldrich) as a magnetic susceptibility matching fluid ...
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bioRxiv - Microbiology 2021Quote: ... gondii parasites were cultured in complete medium (DMEM, +5 % FCS) or in custom culture medium −Pan (DMEM −Pan, +5 % dialyzed FBS) supplemented with Val (Sigma, V6504), pantoate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... in 20 ml Dulbecco’s Modified Eagle Medium (DMEM, Capricorn, Ebsdorfergrund, Germany) containing 10 % fetal calf serum (FCS, Sigma, St. Louis, USA) and 1 % penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Any larger tissue fragments were dissociated mechanically followed by washing of the cell strainer with MACS buffer (PBS, 2% FCS, 2mM EDTA (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...