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Citations for Millipore Sigma :
2901 - 2950 of 4532 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 25 µg of viral plasmid and 100 µl branched PEI (pH 7.4) (Sigma-Aldrich) were added to the cell culture ...
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bioRxiv - Biochemistry 2022Quote: ... The expression plasmid was transformed into Rosetta-Gami®2 (DE3) competent cells (Novagen) to maintain disulfide bond formation ...
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bioRxiv - Microbiology 2021Quote: ... Up to 12 µg linearised plasmid DNA was added to an electroporation cuvette (Sigma), and 100 µL cells were subsequently added ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid vectors were transformed in BL21(DE3) competent cells (Invitrogen or Sigma Aldrich) and grown overnight in a starter culture of 50 mL LB media (Fisher Bioreagents ...
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bioRxiv - Molecular Biology 2019Quote: ... bacterial strain BLR (DE3) was co-transformed with pLacRARE2 plasmid from Rosetta2 strain (Novagen), GST-p125 and pCOLA-hPold234 ...
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bioRxiv - Molecular Biology 2019Quote: ... WT and mutated DEF6 plasmids were transfected (Gene-juice Transfection Reagent, Novagen, #70967-3) into COS7 cells and 24h after the transfection ...
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bioRxiv - Developmental Biology 2021Quote: Zinc finger nuclease (ZFN) plasmids targeting the LEPTIN gene were designed by Sigma-Aldrich. Fetal fibroblasts were isolated from 30-day old male pig embryo ...
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bioRxiv - Cell Biology 2020Quote: ... We used a previously reported plasmid (McMahon et al., 2012) with the pET21b (Novagen) backbone that expresses a C-terminal truncation of Sec12 (Sec12ΔC ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were sub-cloned into the plasmid expression vector pET-28a (Novagen) previously cut with the restriction enzymes EcoR I and Xho I ...
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bioRxiv - Immunology 2022Quote: ... the plasmid DNA was added with transfection reagent and serum-free DMEM (Sigma-Aldrich,) to cells for 24 h by strictly following manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The above plasmids were co-transfected into brown preadipocytes and selected with puromycin (Sigma). Cells were re-plated without puromycin and single cell clones were examined by genotyping ...
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bioRxiv - Cancer Biology 2024Quote: ... The sgRNA plasmid was then transfected into 4T1 cells using Nanojuice transfection reagent (Millipore). After 48 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were used to transform BL21 (DE3) chemically competent cells (Sigma-Aldrich-Aldrich). After transformation and growing the transformed cells on antibiotics selection agar plates ...
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... EGFP sequence cloned into the pIEx-4 plasmid (Sigma-Aldrich, Saint Louis, MO, USA) served as a negative control for cell line selection.
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bioRxiv - Cell Biology 2024Quote: ... The cloned plasmids were transfected into HEK293T cells using GeneJuice transfecting agent (Merck Millipore). Forty-eight hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasmids were concentrated using an Amicon spin centrifugal filter unit (Millipore Sigma). A small portion of these plasmids was also prepared with 32 Illumina library indexes and sent for sequencing along with the output library.
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bioRxiv - Microbiology 2024Quote: ... The subcultures of strains carrying pBAD33 plasmids were grown with 0.2% arabinose (Sigma-Aldrich). OD600 was measured ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transfected with plasmid encoding Flag-DYRK1B using PEI Prime (Sigma-Aldrich), then 4 h later medium was changed and cells were left for 48 h ...
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bioRxiv - Cell Biology 2023Quote: ... MPP6 and NO66 were PCR cloned from plasmid DNA or cDNA into pFLAG (Sigma), pEF6 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... coli XL-I Blue cells with the Sigma GenElute Plasmid Miniprep Kit (Sigma-Aldrich) as specified by the manufacturer.
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bioRxiv - Genetics 2023Quote: ... plasmids were transformed into Rosetta2(DE3) Plyss SinglesTM competent cells (Millipore Sigma, Catalog #70236), and individual colonies were chosen and optimized for protein expression during the following day ...
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bioRxiv - Physiology 2023Quote: ... All vectors were prepared with the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma). On day 0 ...
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bioRxiv - Biophysics 2023Quote: ... packaging plasmids at a 1:1:1:2 molar ratio using polyethyleneimine (Sigma-Aldrich). Viral supernatant was collected 48 and 72 h post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’-pGEM-T construct was then isolated with GenEluteTM Plasmid miniprep kit (Sigma) and digested with 0.5 µl (10 U ...
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bioRxiv - Developmental Biology 2024Quote: ... Pre-validated CompoZr(R) ZFN plasmids for Il2rg (CKOZFN33739) were purchased from Sigma-Aldrich. Il2rg ZFN mRNAs were synthesized by in vitro transcription with mMESSAGE mMACHINE T7 Ultra kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... primary mouse myoblasts and human immortalized myoblasts were transfected with 60nM final of siRNAs (Sigma Aldrich) using LipoRNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC lines were maintained on irradiated MEFs in hESCs medium [DMEM/F12 (Sigma-Aldrich, D6421) supplemented with 20% KO Serum Replacement (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by i.p injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human alveolar type II epithelial cell line A549 was purchased from Sigma-Aldrich (Poole, UK) and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... which is made by supplementing RPMI Medium 1640 with 5 µg/mL human insulin (Sigma-Aldrich), 5 µg/mL human transferrin (Sigma-Aldrich) ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Immunology 2019Quote: ... they were intraperitoneally injected with 2.5 iu human chorionic gonadotropin (HCG, Sigma Aldrich, St. Louis, MO) and immediately mated with male mice overnight (16 hours) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on glass coverslips coated with 50µg/mL human type IV collagen (Sigma C7521) 24 to 48h before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... META-enriched cultures were isolated via resistance to complement-mediated lysis in 20% human serum (Sigma) for 30min at 37°C and validated via sherp expression [21,23].
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bioRxiv - Immunology 2019Quote: Human and mouse omentum samples were fixed for one hour on ice in 10% NBF (Sigma) and then permeabilized in PBS 1% Triton-X 100 (Sigma ...
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bioRxiv - Genomics 2021Quote: DNA from human keratinocyte cells was extracted using the “GenElute-Mammalian Genomic DNA Miniprep Kit” (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then 50 μL of alkaline phosphatase-conjugated secondary goat anti-Human anti-IgG (Sigma Cat # A9544), anti-IgA (Abcam Cat # AB97212) ...
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bioRxiv - Bioengineering 2021Quote: Immortalized human neural progenitor cells derived from the ventral midbrain (ReNCell VM) were obtained from Millipore. Cells were expanded on laminin-coated tissue culture flasks ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (A1653) and mouse serum albumin (A3139) were purchased from Sigma (St Louis, MO). Fluorescently labeled human serum albumin (HS1-S5-1 ...
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bioRxiv - Neuroscience 2020Quote: ... were co-transfected into U87 human primary glioblastma cells by using ESCORT V transfection reagent (Sigma). The fold increase in relative luciferase activity is a product of the luciferase activity induced by TCF7l2-S2 or TCF7l2-E2 (backbone vector pCMV ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
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bioRxiv - Cell Biology 2021Quote: ... HAP1 cells were transfected with 600 ng/ml human VRK2 MISSION® esiRNA (EHU070731, Sigma-Aldrich) using Lipofectamine RNAiMAX (13778075 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...