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Citations for Millipore Sigma :
2901 - 2950 of 3014 citations for 6 Methoxyquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Molecular Biology 2023Quote: ... The α-FLAG (clone M2, catalog number F1804) and α-glucose-6-phosphate dehydrogenase (G6PDH, catalog number A9521) were purchased from Millipore Sigma. The α-puromycin antibody (clone PMY-2A4 ...
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bioRxiv - Cancer Biology 2023Quote: ... were deparaffinized and then subjected to antigen retrieval for 10 minutes at 121 uC at 15 PSI in 2.94 g/L sodium citrate (pH 6; Sigma Aldrich, 61332-04-3). Sections were permeabilized in PBS with 0.4% Triton X-100 (PBT ...
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bioRxiv - Neuroscience 2023Quote: ... 315-319 mOsm) with a ceramic blade and placed directly onto 6-well Millipore Millicell cell culture inserts (Millipore Sigma PICM0RG50, Burlington, Massachusetts) over 1 mL warmed neuronal media (1x MEM (Millipore-Sigma) ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5-ml glass vials (Ø×H 11.6 × 32 mm) containing ∼ 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC, >98%, Sigma-Aldrich, Buchs, Switzerland) and sealed with screw caps containing a rubber septum ...
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bioRxiv - Genomics 2023Quote: ... whole cell lysates were obtained from ∼80% confluent cells in individual wells of 6-well dishes using RIPA lysis buffer (Sigma Aldrich, R0278-500ML) supplemented with Protease and Phosphatase Inhibitor Mini Tablets (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF) (Sigma-Aldrich, Cat# B3795). Cells were seeded at an initial density of 2 × 104 cells/cm2 in 24-well plate coated with Type I collagen (Corning ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Bioengineering 2024Quote: ... each well was filled with 400 µL of RPMI-B27 supplemented with 10µM Y-27632 and 5 mg/mL 6-aminocaproic acid (Sigma-Aldrich, cat. no. A7824). On day 1 ...
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bioRxiv - Developmental Biology 2024Quote: The para-nitro derivative of blebbistatin was chosen for myosin inhibition experiments due to its non-phototoxic and non-cytotoxic effects. The drug was purchased as lyophilized powder (Motorpharma, cat.no. 1621326-32-6) and dissolved in 100% DMSO (Sigma-Aldrich, cat. no. D8418) to prepare a stock solution of 10mM concentration that is stored at -200 C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by either a 2 minute (6 µm sections) or 10 minute (50 µm sections) rocking at 37°C in pepsin reagent (ref # R2283, Millipore Sigma, Burlington, MA). The pepsin reaction was quenched by successive incubations in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: Neuronal processes in octopus arm were labeled with mouse monoclonal antibody 6–11B-1 (1:500 dilution of ascites fluid; Sigma-Aldrich, Cat#: T6793) and mouse monoclonal antibody SMI-31 (1:500 dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... and HEK293T embryonic kidney cells [6] were grown in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO). All cells were cultured at 37°C in 5% CO2 and 5% O2 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Bioengineering 2020Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Neuroscience 2021Quote: ... CAS # 7646-85-7 and ZnC4H6O4; CAS #557-34-6) and arsenic (NaAsO2; CAS #7784-46-5) (all from Sigma-Aldrich, St Louis, MO). The metallic compounds were either dissolved in 30% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific, Germany; Sigma-Aldrich, Germany; TPP AG, Switzerland). For analyses using the Operetta high content imaging system cells were plated with 10,000-50,000 cells/ml in 96-well µClear plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and subjected to 6 h dynein inhibition with Dynarrestin (25μM, SML2332-5MG, Sigma-Aldrich, Saint Louis, MI, USA) or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin antibody (Lysine-40) (Sigma Clone 6-11B1), α-tubulin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Neuroscience 2022Quote: ... was performed on a 1 in 6 series of sections from sham- or 6-OHDA-lesioned animal as previously described[57] using an anti-TH mouse monoclonal antibody (LNC1 clone, EMD Millipore, Burlingame, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Immunology 2021Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO). They were then stimulated with peptide pools for 12 h ...
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bioRxiv - Bioengineering 2021Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by stirring for 1 hour in lysis buffer (100 mM Tris, 150 mM NaCl, 10% glycerol, 6 mg/mL lysozyme (Sigma, St. Louis, MO, USA), 2 mg/mL deoxycholic acid ...
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bioRxiv - Cell Biology 2021Quote: ... We used following combinations of primary (dilution 1:200) and secondary (dilution 1:250) antibodies: mouse monoclonal anti-acetylated tubulin (Sigma, [6-11B-1], T7451) with Abberior Star 635P goat anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... medium (HiMedia Laboratories Pvt Ltd.) was filter-sterilized with 0.2 μm filters and supplemented with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA) for the culturing of B ...
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bioRxiv - Immunology 2020Quote: ... Pellet was resuspended in DMEM/F12 media and plated at 250,000 cells/mL on 6-well plates that had been pre-coated with 100 mg/ml PDL (Sigma-Aldrich, cat # 27964-99-4) at 37°C overnight ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stained by a PBS-based staining solution of 2 μM Calcein-AM from Thermo Fischer Scientific (Waltham, USA) for viable cells and 6 μM Ethidium homodimer-1 from Sigma Aldrich (St.Louis, MO, USA) for dead cells for 30 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... the sperm pellet was washed in IVF–TALP medium and a final concentration of 1 × 106 spermatozoa/mL was adjusted using IVF–TALP medium enriched with BSA (Sigma A8806; 6 mg/mL) and heparin (25 mg/mL).
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bioRxiv - Microbiology 2022Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 supplemented with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO) at 37°C followed by stimulation with peptide pools for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... 6-Carboxyfluorescein filled liposomes were separated from the unincorporated 6-Carboxyfluorescein by size exclusion chromatography using Sephadex G 25–300 (Sigma Aldrich, Saint Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Physiology 2024Quote: ... Nuclear stains used in this study were 4’,6-diamidino-2-phenylindole (DAPI; final concentration 1 μg/ml Sigma-Aldrich, St. Louis, MO, USA). F-Actin was stained with Alexa Fluor 568 phalloidin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: Non-cornmeal molasses food was freshly made with or without 100 µM or 5 mM Phloroglucinol (PHG) (Sigma-Aldrich, CAS number 108-73-6) and solidified in a vial overnight ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with DPBS five times for 3 min each, and stained with 4’, 6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS) (Sigma, San Francisco, CA, USA) in the dark at room temperature for 8 min ...
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bioRxiv - Neuroscience 2024Quote: ... One group of mice (referred to as DMI + 6-OHDA) was pre-treated with one injection of desipramine hydrochloride (DMI, Sigma-Aldrich, 25mg/kg i.p.) 30 minutes before the 6-OHDA infusion in order to protect the noradrenergic system [50].