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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mice were treated with the non-selective NOS inhibitor N(G)-Nitro-L-arginine methyl ester (LNAME, Sigma). LNAME (10mg/ml made up with sterile saline ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... hydroxyl and carboxyl groups were modified with 80 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) (Sigma-Aldrich) with 1% trimethyl-chlorosilane (TMCS ...
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bioRxiv - Immunology 2020Quote: ... and the protein was eluted with MES wash buffer supplemented with 500 mM methyl-alpha-D-mannopyranoside (Sigma). The protein was buffer exchanged into phosphate buffered saline by successive rounds of centrifugation and stored at −80°C.
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bioRxiv - Microbiology 2021Quote: ... N-methyl-trimethyl-silyl-trifluoroacetamide (MSTFA) and methoxyamine pyridine hydrochloride were of chromatographic grade and purchased from Sigma–Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... in gels prepared with 0.1 % (w/v) of pectin from apple (with 50-70 % methyl-esterification, Sigma-Aldrich), 1 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Major peaks were identified by retention time relative to a Bacterial Acid Methyl Ester (BAME) standard (Millipore-Sigma) and a 37 FAME standard (Supelco) ...
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bioRxiv - Microbiology 2022Quote: ... Major peaks were identified by retention time relative to a Bacterial Acid Methyl Ester (BAME) standard (Millipore-Sigma) and a 37 FAME standard (Supelco) ...
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bioRxiv - Microbiology 2023Quote: ... opening of the mitochondrial permeabilization transition pore (mPTP) was visualized by tetramethylrhodamine methyl ester perchlorate (TMRM) staining (Sigma). Cells were washed once with 1 x HBBS (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... neurotoxic lesions in ACC were made by infusing N- methyl-D-aspartate (NMDA, Sigma-Aldrich, St. Louis, MO) in a phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... dried samples were resuspended in 162.5 µl hexane and 37.5 µl N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) (Sigma-Aldrich) and incubated at 37 °C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... we used Nω-Nitro-L-arginine methyl ester hydrochloride (L–NAME, 2.5 mM, Sigma, Cat. No. N5751-10G); the NOS2 inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... was dissolved in buffer with and without Heptakis(2,6-di-O-methyl)-β-cyclodextrin (Sigma-Aldrich, 39915-1G) (1% w/v ...
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bioRxiv - Biochemistry 2024Quote: ... the medium was changed to DMEM containing 10% FBS and 1 mM Methyl-o- nitropiperonyllysine (mNPK, Sigma-Aldrich) for #2-#4 ...
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bioRxiv - Immunology 2023Quote: ... spleen cells were pre-treated with 20 mM of the cholesterol-sequestering agent methyl-beta-cyclodextrin (MβCD, Sigma) for 4h ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... followed by alkylation at ambient temperature for 30 minutes with 20 mm S-Methyl methanethiosulfonate (MMTS, 64306 Sigma). Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin ...
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bioRxiv - Neuroscience 2024Quote: Mice were treated with 12 mg/kg of L-3,4-dihydroxyphenylalanine methyl ester hydrochloride (L-DOPA, Sigma Aldrich) combined with 12 mg/kg of benserazide (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Genomics 2020Quote: ... cleared by centrifugation at 1500rpm for 5 minutes at 4⁰C and then passed through a 0.45um pore PVDF Millex-HV (Millipore). Lentivirus was aliquoted and stored at −80⁰C for future use.
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was centrifuged in a Beckman JA25.50 rotor at 16,000 rpm for 30 min at 4°C and 8.4 mL 5% neutralized PEI (Sigma P3143) was added dropwise into the supernatant with gentle agitation for 15 minutes at 4°C followed by another centrifuge at 13,000 rpm for 10 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... The spinal cords were dissected and fixed in 4% paraformaldehyde (PFA) and 5% saturated picric acid (Sigma-Aldrich, P6744) in phosphate buffer saline (PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 40ul of 20mg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside – Sigma Aldrich B44252) and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773 ...
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bioRxiv - Microbiology 2021Quote: ... 35x volume per mg) by vortexing vigorously for 5 min using 4 mm glass beads (ColiRollersTM Plating beads, Novagen), followed by incubation in a sonication bath for 10 min and shaking at 1,200 rpm for 10 min in a Thermomixer with a thermo block for reaction tubes ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed in 10% buffered formalin (4% formaldehyde) for 5 min at room temperature and permeabilized for 30 min in PBS containing 0.1% saponine (Sigma) and 1% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... gonads were dissected from 4 and 5 day old female jellyfish in 400 µM menthol in seawater (Sigma-Aldrich, #M2772 ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was changed to RPMI + B27-I with 5 μM Wnt inhibitor IWP-4 (SML1114, Sigma-Aldrich, USA) and incubated for 48 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 4-hour decalcification in the same fixative with addition of 5 % EDTA (ethylenediaminetetraacetic acid, Sigma-Aldrich) at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... 1000 rpm continuous shaking at 4 °C) with 100 μL elution buffer (6.7 mM m6A 5-monophosphate sodium salt [Sigma # M2780-10MG] ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Immunology 2020Quote: ... 1 mL of lentiviral supernatant was added to 5×105 RAW 264.7 macrophages with 4 μg/mL of hexadimethrine bromide (Millipore) and cells were spinoculated at 300 x g for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
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bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoprecipitation was carried out by rotating sonicated lysates overnight at 4 C with NFIA antibody (5 mg, Sigma, HPA006111) or SOX9 antibody (EMD Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid ...